Lab 1 Assignment

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Carleton University *

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Oct 30, 2023

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Fall 2023 Lab 1 - The Microbiology of River Water BIOL 1103 Lab 1 Experimental Assignment Full Name: Ali Khelil Student #: 101258560 Lab section: Thursday 1:35 A14 Although the experimental work will be completed in pairs, all work submitted must be completed individually. It is important that you do not copy from the lab manual or from each other. DO NOT plagiarize! Remember to properly cite and reference any external sources used (refer to Appendix 1 for guidelines). Unless otherwise specified in the question, all answers should be written using complete sentences, paragraph style. Part I: Observing the Microbiome of Raw River Water 1. Using your unaided eye, briefly describe the raw river water as if you were making observations for a lab report (3 marks) . The water was tainted with a brownish green colour compared to the filtered water which was clear. There were remains of debris in the water. 2. Using complete sentences, write a detailed description of the general characteristics of the organism that you will draw (in Question 3) from the raw river water. Include details regarding the shape, texture, and colour of the specimen. Describe the general thickness of the cell wall and/or the plasma membrane and whether any organelles were visible (3 marks) . The organism that I have found from the raw river water was very big even under the 10X scope. Looking at the organism there were a lot of spots, and there were lines all along the body. The eyes were in an oval shape and the only black part of the organism. The organism had a sandy colour to it. 3. Create a detailed diagram of the specimen that you described in Question 2 (3 marks) . (You may insert an image of your lab notebook diagram, provided it is in the correct format, see Appendix 2). 1
Fall 2023 Lab 1 - The Microbiology of River Water BIOL 1103 4. In the space below, calculate the actual length of your specimen from Question 3 and its magnification based off of your figure. Show all calculations and work (4 marks) . 2
Fall 2023 Lab 1 - The Microbiology of River Water BIOL 1103 Actual Specimen Length Figure Magnification o.u.=125 1.0 um/o.u. x 125 o.u. =125 um 10X 5. State a correct title for your drawing as if it were going to be included in a formal lab report (1 mark) . Figure 1. whole mount of unknown organism using a 10x scope 6. Where would the title be placed relative to your drawing (i.e. above, below, or to the side of your figure) (1 mark) ? The title would be placed below the figure. Part II: Comparing and Observing the Microbiome of the Filtered River Water 7. Compare the appearances of the raw and filtered river water. a. Are there any differences that can be seen with the unaided eye (1 mark) ? You can see the difference in colour between the 2 different types of water and you can also see what is floating around in the raw river water. b. Are there any differences that can be seen using the microscope (1 mark) ? You can see all the tiny organisms that would normally be swimming around in the raw river water. You would also see things that you can’t see with the naked eye in both the filtered water and the raw river water. c. Was this the result you expected? Briefly describe why or why not (2 marks) . That was the result I expected because the filtered water would remove the excess items that you can see with the naked eye but not the tiny organisms that 3
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