Post-Lab 2 Assignment

Question: Please help me explain why tubes 1 and 3 showed evidence of unhydrolyzed strach after 30 minutes. Procedure and data sheet are already given

topic: Isoelectric Precipitation   Appearance of the mixtures containing casein mixed with different buffer solutions: (a) TestTube 1 containing Glycine-HCl buffer; (b) Test Tube 2 containing Acetate buffer; and (c) Test Tube 3containing Phosphate buffer. QUESTION: Based on the given results, what do you think is the isoelectric pH of casein? Briefly discuss your basis for determining it.

Protein Agarose Gel Electrophoresis Objectives: At the end of the exercise, you should be able to1. Understand the concepts of protein structures, isoelectric point of amino acids andproteins.2. Understand the principle of separation in protein agarose gel electrophoresis.3. Demonstrate laboratory techniques used in running a protein agarose gel (preparinggels, loading samples with micropippets, and electrophoresis).4. Understand the molecular difference between normal and mutant forms of betahemoglobin in patients with sickle cell disease, and how protein agarose gelelectrophoresis can be used in disease diagnosis. Comparing Migration Rates of Hemoglobin Isolated from Normal, Heterozygousand Sickle cell anemia Individuals at pH 9.2: Part B: pH 9.2, 1X buffer Materials on the supply bench:1) gel running apparatus (lid matches the tank); one comb.2) flasks for preparing agarose gel (125 ml)3) micropippets (P20) and yellow tip boxes4) plastic waste cup, markers and microcentrifuge…

Please calculate the denaturation percentage

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Time remaining: 01:51:42 logy Remaining Time: 1 hour, 18 minutes, 23 sec Ooestion Completion Status: QUES TION Name the phase of this reaction and Label the diagram Aote fand I are locations For the f bar, press ALT+F 10 (PC) or ALT+FN+F10 (Mac) BIUS Paragraph Arial Click Save and Submit to save and submit. Click Save All Answers to save a

Materials - 2021FA-CHM-1O X Bb 4427577 d-fleet02-xythos.content.blackboardcdn.com/6086c260d7e8f/4427577?X-Blackboard-Expiration=1633143600000&X-Blackboard-Sig 6 / 9 100% Exercise 1: Standard Curve for Protein Measurements: A standard curve for protein concentration is often created using known concentrations of bovine serum albumin (protein). This process is called the Bradford Assay; it is a colorimetric assay. A special reagent turns blue when it binds to amino acids present in protein. The intensity of the color is best measured with a spectrophotometer (a device for comparing two light radiations, wavelength by wavelength). In the case of the Bradford Assay the greater the absorbance, the higher the protein concentration. A series of tests were performed on some samples and spectrophotometer: following measurements were obtained using a Protein Concentration (mg/ml) Absorbance (A) 0.26 0.098 0.56 0.213 0.383 0.84 1.12 0.473 1.40 0.527 TASKS: 1. Enter the data into Excel - the…

Module 5 Week 1 Assignment: Enzymes. This is a group assignment. You must work with a partner. Please be sure to have joined a group in Canvas and then upload one file for the group. The following kinetic data were obtained for an enzyme in the absence of inhibitor (1) or in the presence of two different inhibitors (2 and 3). The Enzyme concentration was the same in all of the experiments. Graph these data as a Lineweaver-Burke plot. Enzyme mM Inhibitor 2 [S] 1 Inhibitor 3 2 4 8 12 Enzyme (1) 12 20 a. Paste a photo of your Linewaver-Burke plot here. Plot all three lines on one graph. b. Using the date, fill in the following table; 29 35 40 v (umol/ml *sec) Inhibitor (2) 4.3 8 14 21 26 Vmax Km Apparent Vmax Apparent Km Type of inhibition Apparent Vmax Apparent Km Type of inhibition Inhibitor (3) 5.5 9 13 16 18 Click or tap here to enter text. Click or tap here to enter text. Click or tap here to enter text. Click or tap here to enter text. Click or tap here to enter text. Click or tap…

Exercise 3 The MTT assay is a widely used assay to study cell viability. It measures cellular metabolic activity, which serves an indicator of cell viability, proliferation and cytotoxicity. This non- radioactive colorimetric assay is based on the reduction of a yellow tetrazolium salt (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide or MTT) to purple formazan crystals by metabolically active cells. Viable cells contain NAD(P)H-dependent oxidoreductase enzymes that reduce the MTT to formazan. The insoluble formazan crystals are dissolved and the resulting coloured solution is quantified by measuring absorbance at 500-600nm using a multi-well spectrophotometer. The darker the solution, the greater the number of viable, metabolically active cells! Exercise 3.1 MTT protocol Your colleagues in the School of Biology and Environmental Science discovered an unusual looking green algae on a recent fieldtrip to Costa Rica. They have provided you with some extracts from the plant…

P9-11B The following data on baker's yeast in a particular medium at 23.4°C were obtained in the presence and in the absence of an inhibitor, sulfanilamide. The reaction rate (-3) was measured in terms of the oxygen uptake rate Qo, obtained as a function of oxygen partial pressure. (a) Assume the rate Qo, follows Michaelis-Menten kinetics with respect to oxygen. Calcu- late the lo, maximum (i.e., Vmax), and the Michaelis-Menten constant KM- (Ans.: V = 52.63 µL O,/h-mg cells.) (b) Using the Lineweaver-Burk plot, determine the type of inhibition sulfanilamide that causes the O₂ uptake to change. Po, 05 05 35 5.0 Qo, (no sulfanilamide) 00 23.5 33.0 375 42.0 43.0 Qo, (20 mg sulfanilamide/mL added to medium) Page 428 / 993 0.0. 25.6 30.8 36.4 39.6 40.0 Po₂ oxygen partial pressure, mmHg. Qo, = oxygen uptake rate, µL of O₂ per hour per mg of cells. (c) List ways you can work this problem incorrectly. (d) Apply one or more of the six ideas in Preface Table P-4, page xxviii, to this problem. Q+

567write true if the statement is correct, otherwise, change the underlined word/phrase to make it correct. do not use abbreviations please answer all

III. Problem solving. Show your computations. Answer questions briefly. A 50.0 ml juice extract is colorimetrically assayed using Nelson's test. One milliliter (1.00 mL) of the solution and the standard glucose solution (concentration: 1mg/mL) were treated with freshly prepared Nelson's reagent and arsenomolybdate reagent and then diluted to 10.0 mL separately in properly labeled test tubes. Absorbance at 480 nm for the standard is 1.702 and for the sample, 0.926. A. What is the principle behind the assay?

After three minutes, the concentration of drug Zip in the red blood cells is 10 mmoles l-1. What is the average rate of entry of drug Zip into the red blood cells in units of moles min-1 red blood cell-1 during the first three minutes after placing the red blood cells into the bathing solution? Assume that each red blood cell occupies about 1 x 10-13 liters.

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9:58 ← LTE 43% PHGY 215 Applied Physi... Questions: 1. Using Table 3, which contains the normal intracellular ion concentrations for most cells in the body, calculate the equilibrium potential for each of the four ions for normal intracellular and normal extracellular ion concentrations. Make sure to show your calculations. Repeat these same calculations, only this time utilize the hyperkalemia values from Table 1. Table 3. Intracellular ion concentrations. lon Intracellular concentration (mM) 150 K+ Na* 15 CI Ca2+ 4 .0001 2. Using Table 4, which contains the relative ion permeabilities for neurons and cardiac myocytes at rest, calculate the resting membrane potentials for both neurons and cardiac myocytes. Make sure to include calculations for both hyperkalemia and normal plasma ion concentrations. Table 4. Relative ion permeabilities for neurons and cardiac myocytes. lon K+ Na+ Neuron relative permeability 1 .04 CI- .45 Cardiac myocyte relative permeability 1 0 0 3. Using Table 2,…

Question: Determine the amount of dehydrated medium needed to prepare 15 butts, 20 slants, and 25 butt-slants. Include amount for 2 additional units of each as excess to compensate for compounding losses.

INPUT AND OUTPUT COMPUTATION

Match the number in the image with the correct name for the lab supply 1 2 ♡ AGEN QIAGEN QIAGEN QIAC RIVCEM БИЗӘДІС RNeasy Mini Kit (50) Cat. No. 74104 Store at room temperature (15-25°C) WEZAIO QIAGEN 2 GDIVERM QIAGL K (20) QIAGL умени DAIO QIAG GAGEN GIVCEN QIAGEN QIAGEN ИЗОМО QIAGEN NEA QIAGEN QIAGEN N QIAGEN QIAGE Buffer W AGEN Lysis buffer 45 m Buffer RPE Wash buffer 11 ml concentrate to ustan 5 m Butter R U 3 clear collection tube centrifuge tube pink column (with filter) PhaseLock tube pink column (with filter) inside a clear collection tube <

16. Your Excel data graph for Serial Dilution lab:** 16a. Include a clear and labeled graph of DF (x axis) versus Absorbance (y axis). See box above for expectations and review video tutorial of this Excel work. Do not graph two of your data points: your blank or your Cuvette #1 (DF=1). 1 Q A Multiple Styles W S X 3 E D $ 4 To C R F % 5 I T V U ^ 6 G Y 7 H U 8 J BN S ( 9 K M X

Reminder : ONLY ONE GRAPH

Only 13.40

SOMOGYI-NELSON Method(clinical chemistry) a. What is the type of blood sample used and how much of it is added to distilled water? b. How much distilled water was used in the procedure? c. What are the protein precipitants used and indicate the specific concentration of each? d. What is the color of the precipitate?

Q26

flow chart of purification steps of Beta-fructofruanosidase (Invertase)

Time left 0:4T:30 estion In the experiment of amylase extraction from barely seeds, after the second centrifugation- the amylase enzyme will be found in A yet vered ked out of Flag -stion B O a. B: Precipitate O b. A: Supernatant O c. B: Supernatant O d. A: Precipitate ENG E iSall zh DELL

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student question 1. How do both Hemoglobin and S-adenosylmethionine synthetase form hydrophobic pockets? explain in detail. 2. how does the structure of S-adenosylmethionine synthetase make it resistant to heat denaturation and why, explain in detail