Post-Lab 2 Assignment

 The absorbance value of the 1000x sample is negative because it is larger than the y- intercept on the standard curve (y-int = 1.0871), so it falls outside the scope of the assay. This means that the amylase became too dilutes and no/very little starch was broken down, resulting in a high absorbance value (iodine had lots of starch to react with).  The 100x solution was too concentrated with amylase while the 1000 solution was too diluted to calculate the actual concentration of amylase in the saliva. Your saliva sample Amylase Conc (ug/mL) Amylase Conc (mg/mL) 10x, 100x and 1000x Y=mx+b -0.293849 3. Using the completed data Table 2.8 provided by your TA, with both amylase concentration and self-reported starch consumption from all your lab mates, create a 3-bar graph. Create as a publication-grade figure using 3 bars (low, moderate, high starch consumption on the x-axis and amylase concentration on the y-axis) with accompanying error bars. You will be creating two versions of the same figure. The data will remain the same, but one figure will have standard deviation error bars and the other figure will have range error bars. Instructions on how to do this assignment is detailed in Excel Tutorial #3 for Office 365 - Creating a Bar Graph with Error Bars (p. 119) (10 marks)

Some of the Set 3 tubes turn a darker colour due to the amount of starch that remains in the solution. Iodine reacts with the remaining starch, producing the dark blue colour. The more starch that remains in the solution, the darker blue the solution turns. Starch Diet Amylase concentraion (mg/mL) High 0.73 High 4.41 High 33.9 High 0.513 High 1.78 High 3.14 High 5.77 High 1.67 High 2.21 High 5.31 High 6.23 High 5.98 Low 2.1 Low 0.644 Low 0.376 Low 2.96 Low 1.54 Low 2.07 Low 3.29 Low 1.87 Moderate 4.752 Moderate 0.988 Moderate 1.72 Moderate 1.66 Moderate 1.89 Moderate 0.739 Moderate 1.524 Moderate 0.748 Moderate 4.55 Moderate 5.78 Average Amylase Concentration in High Starch Diet Standard Deviation for High Starch Diet 5.97025 9.037753383 Average Amylase Concentration in Moderate Starch Diet Standard Deviation for Moderate Starch Diet 2.4351 1.85848591