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Biotechnology AP.BIO: IST-1 (EU), IST-1.P (LO), IST-1.P.1 (EK) [zl Google Classroom Kl Facebook W Twitter £ Email The flavonoid 3 -hydroxylase (f3 'h) gene influences flower color in common morning glory plants. A researcher collected DNA from common morning glory plants and used DNA sequencing to identify the order of nucleotides in the f3 'h gene for each plant. A subset of the sequencing data, which shows the results for two plants, is shown in Figure 1. Plant 1: ATGGCTACCTTA Position: 1 2 3 4 5 6 7 8 9 1011 12 Plant 2: ATGCCTGCCTTA 1l Position: 1 2 3 4 5 6 7 8 9 1011 12 Figure 1: DNA sequencing data for positions 1-12 of the f3’h gene in two plants. Which of the following can be determined from the data in Figure 1? Choose 1 answer: ° CORRECT (SELECTED) Plant 1 and Plant 2 differ in at least two nucleotides of the f3'h gene. Figure 1 shows a type of DNA sequencing data where each colored peak represents the detection of a single nucleotide in a DNA sequence. According to the data, the nucleotides at R S RS I S5 2N TN L7 N SN N D —_— Y SR R T upe R W ] .

Which of the following can be determined from the data in Figure 1? Choose 1 answer: ° CORRECT (SELECTED) Plant 1 and Plant 2 differ in at least two nucleotides of the f3'h gene. Figure 1 shows a type of DNA sequencing data where each colored peak represents the detection of a single nucleotide in a DNA sequence. According to the data, the nucleotides at positions 4 and 7 in the sequence differ between the plants: Plant 1 has a G and an A, while Plant 2 has a C and a G. So, based on the data in Figure 1, we can determine that Plant 1 and Plant 2 differ in at least two nucleotides of the f3’h gene. @ INCORRECT Plant 1 does not produce a functional F3'H protein, while Plant 2 does. Figure 1 shows a type of DNA sequencing data where each colored peak represents the detection of a single nucleotide in a DNA sequence. Using this data we can see that Plant 1 and Plant 2 have nucleotide differences at positions 4 and 7 of the f3’h gene. But, we need more information about the sequence of the gene in order to determine how this nucleotide difference might affect protein function. @ INCORRECT Plant 1 and Plant 2 have identical genomes. Figure 1 shows a type of DNA sequencing data where each colored peak represents the detection of a single nucleotide in a DNA sequence. Using this data, we can see that Plant 1 and Plant 2 have nucleotide differences at positions 4 and 7 of the f3’h gene. Because of these differences, we know that Plant 1 and Plant 2 do not have identical genomes. @ INCORRECT Plant 1 makes more flowers than Plant 2 does. Figure 1 shows a type of DNA sequencing data where each colored peak represents the detection of a single nucleotide in a DNA sequence. Using this data we can see that Plant 1 and Plant 2 have nucleotide differences at positions 4 and 7 of the f3’h gene. We need more information about the gene to predict whether this change might impact the protein function, and therefore the flower phenotype. Either way, this difference is unlikely to affect flower number because, according to the text, the f3’h gene influences flower color. Way to go! Review related articles/videos. Report a problem

A scientist used laboratory techniques to move Gene X from a bacteria-specific plasmid into a yeast- specific plasmid. To do this, the scientist first used restriction enzymes to digest, or cut, the bacteria- specific plasmid on either side of the gene, which resulted in a mixture of two linear pieces of DNA (Figure 1). Gene X Restriction enzyme digestion fi 1,890 bp I ) 686 bp Figure 1. Diagram showing the bacterial plasmid before and after restriction enzyme digestion (bp = base pairs). The scientist used gel electrophoresis to separate the resulting DNA fragments by size. Then, the scientist cut out the area of the gel containing Gene X in order to purify the gene from the gel. The following diagram shows the digested plasmid DNA as visualized by gel electrophoresis, with various bands labeled A, B, and C. Which of the bands did the scientist cut out in order to isolate Gene X DNA? Choose 1 answer:

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