Lab 1 Assignment (4)

BIOLOGY 126 – PHYSIOLOGICAL BASIS OF LIFE LAB 1 – STANDARD CURVE V 1 C 1 = V 2 C 2 V 1 = (0.1 M ) (30mL) V 1 = 3mL 0.4M 0.4 V 1 = (0.1) (30mL) V 1 = 7.5mL 0.4 /1 6. Fill in the following table. Make sure to add appropriate units to the columns. (5 marks) Table 2. Absorbance of different concentrations of CuSO 4 solutions. Cuvette number CuSO 4 concentration (M) Volume of CuSO 4 ( mL ) Volume of water ( mL ) A 700 1 0.0 0 5 0 2 0.02 1 4 0.104 3 0.04 2 3 0.235 4 0.06 3 2 0.357 5 0.08 4 1 0.532 6 0.1 5 0 0.671 Unknown 0.39 7. In the space below, show a sample calculation for the concentration of CuSO 4 in Cuvette #2. (2 marks) C 1 V 1 = C 2 V 2 V 1 = (0.02M) (5mL) V 1 = 0.1mL 0.1M 0.1 C 1 = 0.1M V 1 = (0.02) (5mL) V 1 = 1mL V 1 = ? 0.1 C 2 = 0.02M V 2 = 5mL /2 8. Using the CuSO 4 solution concentration and absorbance data from Table 2 above, create a properly formatted standard curve and paste into the space below. Refer back to the formatting requirements Page 3 of 4