Effects of Substrate Concentration and pH on Enzyme Activity

Post-lab 4 Report Name: Presley Aaron Sec7on: 501 Claims (Refer back to team component assignment for hypothesis and predic6ons for exercises 4.1 and 4.2) Evidence A. In complete sentences, summarize the class results for Exercises 4.1 & 4.2. Include the range of values for both the independent and dependent variables and any trends that describe how the enzyme ac6vity responded to changes in substrate concentra6on and pH? Include units and report the average values for BOTH exercises. B. Copy/paste class summary data table and graph for Exercise 4.1. Table 4.1 Effects of substrate concentra6on on enzyme ac6vity Class summary of B-galactosidase enzyme ac6vity (nM/min) using ONPG as a calorimetric substrate. Total reac6on 6mes were 10 min. C. Copy/paste class summary data table and graph for Exercise 4.2. Using ONPG as a calorimetric substrate. Total reac6on 6mes were 10 min . Substrate concentra tion (mM) 0 0.2 1.0 2.0 4.0 Mean 0 182.314 489.098 595.594 713.723 N 0 6 6 6 6 pH value 3 5 7 9 Mean 468.878 517.813 344.240 184.082 N 6 6 6 6 The class results for 4.1 shows that as the ONPG concentration increases the bGal activity will increase. The data for exercise 4.2 shows that the higher the pH the lower the enzyme activity will be. The Range for the independent variable In 4.1 is 713.723mM and the range for the dependent variable in 4.1 is 4. The range for the independent variable in 4.2 is 333.731 mM and the range for the dependent variable in 4.2 is 6.

Reasoning Based on the class data, interpret the results to formulate conclusions. Explain evidence that is for or against differences between treatments. Discuss the rela6onship between the independent and dependent variables (e.g. was there a consistent increase). Do your conclusions support or reject your original hypotheses/predic6ons and explain why or why not? What do you conclude about the effects of substrate concentra6on and pH on the ac6vity of β- galactosidase? After viewing the data of 4.1 I can come to the conclusion that substrate concentration increases enzyme activity. We see lower concentrations like 0.2 and 1 have activity between 180 and 480. With higher concentrations like 2 and 4 they have means of closer to 600 and 700. This shows the higher the concentration the higher the enzyme activity. This supports my hypothesis. When looking at 4.2 our hypothesis is also supported by the data. We can see that when the pH is increased the means drop. When the pH is high like 7 or 9 we see lower averages like 340 and 180. When the pH is lower, 3 and 5, the means are closer to 470 and 520.

Post-lab 4 Report Grading Rubric (10 pts total) – To be filled out by the instructor Points Possibl e Points Earned Instructor Comments Claims (2 pts) Hypothesis Is a logical, testable hypothesis clearly stated and based on prior knowledge/observa6ons? 1 (see Team Component assignment) Predic.on Are one or more logical predic6ons clearly stated that suggest a specific outcome for the experiment? 1 (see Team Component assignment) Evidence (5 pts) Methods Are the materials and methods of each experiment thoroughly summarized in complete sentences? 1 (see Team Component assignment) Variables/Groups Are the correct independent and dependent variables clearly stated? Are the control groups explained? 1 (see Team Component assignment) Results Are detailed results reported for each treatment level of the independent variable in both experiments? These results should be summarized in complete sentences. Are actual values reported as class averages with units? 1 Tables/Figures Are the correct tables and graphs presented with proper axes labels and units? Tables and graphs should be accurate and based on the class data . 2 Reasoning (3 pts) Interpret Evidence Are the results correctly interpreted to form logical conclusions? 1 Interpret Claims Is it clearly and logically stated why the results “support” or “refute” the hypothesis and predic6ons? 1 Suppor.ng Evidence Is SPECIFIC suppor6ng evidence provided such as results and interpreta6ons of graphs. 1 Total 10

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Lab 4A Post-lab

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