BIO D7 Population Evolution Genetics

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Biology

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Dec 6, 2023

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Discussion 7 Bio 305 - Genetics Winter 2022 Population and Evolutionary Genetics Part 1 1. Association Mapping . You are investigating the genetic basis of cholesterol level in human populations. You measure the blood cholesterol level in 10 individuals shown below. Each individual is genotyped for molecular markers M1-M11. Each marker has two possible genotypes, which you decide to record as 0 or 1. (For example, a = 0 and A = 1.) Your data are summarized in the table below (assume only one allele is shown and that the other allele is 0 at all positions, in all individuals). cholestero l M1 M2 M3 M4 M5 M6 M7 M8 M9 M10 M11 172 0 1 1 1 1 1 1 1 0 1 0 168 1 1 0 0 1 0 0 1 1 0 1 175 0 0 0 0 1 1 0 0 0 0 0 180 1 0 0 1 1 1 1 0 1 0 1 182 1 1 1 1 0 1 1 1 1 1 1 256 0 0 1 0 0 0 0 0 0 1 1 255 1 0 0 0 0 0 0 0 1 0 0 270 0 1 1 0 0 0 0 1 0 1 0 240 0 1 1 1 0 1 1 1 0 1 1 286 1 1 0 1 1 0 1 1 1 0 1 For each marker, compute the average phenotype of the two genotypic classes. Plot these data on the graph below. (Data for marker 1 is shown.) Marker Genotype M1 M2 M3 M4 M5 M6 M7 M8 M9 M10 M11 Given the following map of molecular markers, indicate the most likely location of the ONE QTL in this region: 1 150 170 190 210 230 250 270 290 0 1 M1 M2 M3 M4 M5 M6 M7 M8 M9 M10 M11
Part 2: HWE 2. You identify a population of mice ( Peromyscus maniculatus ) on an island. Their coat color is controlled by a single gene: BB mice are black, Bb mice are gray, and bb mice are white. You take a census of the population and record the following numbers of mice. Black 200 Gray 160 White 40 a) What are the frequencies of the two alleles? B = 0.7, b = 0.3 b) What are the Hardy-Weinberg equilibrium frequencies for these three phenotypes? p^2 + 2pq + q^2 = .49n + 0.42n + 0.09n = 392 black, 336 grey, 72 white .49 black, .42 grey, .09 white c) A heat wave hits the island. All 200 mice with black fur die from heat stroke, but the other mice survive. What are the new allele frequencies for the population? 0.4 = B, .6 = b d) If the population suffers no further cataclysms after the heat wave, and the surviving animals mate randomly, what will be the frequency of mice with black fur in the next generation? P^2 = 0.16 3. Ten percent of the males of a large and randomly mating population are color-blind (X-linked recessive trait). A representative group of 250 people (males and females) from this population migrates to a South Pacific island, where there are already 1000 inhabitants and where 30% of males are color-blind. Assuming that the initial populations are in Hardy-Weinberg equilibrium, and that there are no further violations of HWE, what fraction of males and females can be expected to be color- blind in the generation immediately after the arrival of the migrants? 25 colour blind, 300 colour blind 325 colour blind 0.26 P = 0.74, q = 0.26 0.0676 4. If 4 percent of a population in equilibrium expresses a recessive trait, what is the probability that the offspring of two individuals who do not express the trait will express it? q^2 = 0.04 q = 0.2 2pq = (0.8)(0.2)(2) = 0.32 .32*.32*.25 = 0.0256
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