Lab Report C

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School

Seneca College *

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Course

573

Subject

Biology

Date

Apr 3, 2024

Type

docx

Pages

8

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Report
Purpose: The purpose of the labs was to study evolutionary biology and protein structure prediction. Lab 6 focused on developing a phylogenetic tree manually using representatives from the three domains of living organisms and a molecular clock to measure evolutionary distance. Lab 7 focused on building a phylogenetic tree using the UPGMA clustering method and visualizing the tree. The protein sequences were obtained from NCBI, saved in FASTA format in a file, and the distance matrix was calculated. Lab 8 focuses on using homology modeling to predict the 3D structure of a drug-resistant mutant HIV protease and comparing it to the native HIV protease, using programs such as RCSB Protein Data Bank, FirstGlance, SWISS-MODEL, and RCSB Pairwise Structure Alignment. These exercises aim to teach important concepts and techniques related to evolutionary biology and protein structure prediction, which have applications in various fields, including medicine and drug design. Method: This method employs homology modeling to forecast the 3D configuration of a drug-resistant mutant HIV protease. The resulting predicted 3D structure is compared with the structure of the native HIV protease to investigate structure-function relationships. To accomplish this, various programs linked to protein structure prediction are employed: RCSB Protein Data Bank (PDB): PDB contains the information about the 3D structures of proteins, nucleic acids, and complex assemblies (i.e., macromolecules). Go to PDB website by clicking the following link or typed in PDB database on google. https://www.rcsb.org/ Search for PDB ID: 1KJF and 1ODW and Download the PDB formatted files.
FirstGlance: FirstGlance shows the Visualization of macromolecules. Go to FirstGlance website by clicking the following link: l https://www.bioinformatics.org/firstglance/fgij/ Navigate to FirstGlance and enter PDB ID: 1KJF to view its 3D structure. Swiss-Model: Homology modeling is one of the computational structure prediction methods that are used to determine protein 3D structure from its amino acid sequence. Download the FASTA-formatted amino acid sequence of the mutant HIV protease. Go to SWISS-MODEL website by clicking the following link or search Swiss-Model on google and Start Modelling. https://swissmodel.expasy.org/ Using User Template input mode, upload the mutant HIV protease amino acid sequence file as the target sequence and upload the 1ODW PDB file as the template file. Click Build Model. Download the resulting model’s PDB file.
RCSB PAIRWISE STRUCTURE ALIGNMENT Pairwise Sequence Alignment is used to identify regions of similarity that may indicate functional, structural and/or evolutionary relationships between two biological sequences
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