dna tester

The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also of hair found at a crime scence left by the murderer [***]. This analysis involves the PCR amplification of SSR loci, each from a different (nonhomologus) chromosome. The PCR primers are for each SSR locus are labeled with a unique fluorescent molecule. Some bands are thicker because relatively more of the corresponding PCR product was obtained. The figure has dots aligned on both sides to help you find the crucial bands; it will help to use a straight-edge as a guide. The numbers at right are the total number of copies of the SSR locus among the population of 11 samples. Are any of individuals 1-10 the murderer? If so, which one? AreanyofthelociontheXchromosome?Ifso,identifythis(these)locus (loci) by color. Are any of the loci on the Y chromosome? If so, identify this (these) locus (loci) by color. Areanyofindividuals1-10probablerelativesofthemurderer?Ifso,identify this person…

The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also of hair found at a crime scence left by the murderer [***]. This analysis involves the PCR amplification of SSR loci, each from a different (nonhomologus) chromosome. The PCR primers are for each SSR locus are labeled with a unique fluorescent molecule. Some bands are thicker because relatively more of the corresponding PCR product was obtained. The figure has dots aligned on both sides to help you find the crucial bands; it will help to use a straight-edge as a guide. The numbers at right are the total number of copies of the SSR locus among the population of 11 samples. Are any of individuals 1-10 probable relatives of the murderer? If so, identify this person and describe the degree of relationship to the criminal.

The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also of hair found at a crime scence left by the murderer [***]. This analysis involves the PCR amplification of SSR loci, each from a different (nonhomologus) chromosome. The PCR primers are for each SSR locus are labeled with a unique fluorescent molecule. Some bands are thicker because relatively more of the corresponding PCR product was obtained. The figure has dots aligned on both sides to help you find the crucial bands; it will help to use a straight-edge as a guide. The numbers at right are the total number of copies of the SSR locus among the population of 11 samples. Are any of the loci on the Y chromosome? If so, identify this (these) locus (loci) by color.

The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also of hair found at a crime scence left by the murderer [***]. This analysis involves the PCR amplification of SSR loci, each from a different (nonhomologus) chromosome. The PCR primers are for each SSR locus are labeled with a unique fluorescent molecule. Some bands are thicker because relatively more of the corresponding PCR product was obtained. The figure has dots aligned on both sides to help you find the crucial bands; it will help to use a straight-edge as a guide. The numbers at right are the total number of copies of the SSR locus among the population of 11 samples. Are any of the loci on the X chromosome? If so, identify this (these) locus (loci) by color.

The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also of hair found at a crime scence left by the murderer [***]. This analysis involves the PCR amplification of SSR loci, each from a different (nonhomologus) chromosome. The PCR primers are for each SSR locus are labeled with a unique fluorescent molecule. Some bands are thicker because relatively more of the corresponding PCR product was obtained. The figure has dots aligned on both sides to help you find the crucial bands; it will help to use a straight-edge as a guide. The numbers at right are the total number of copies of the SSR locus among the population of 11 samples. e. What is the probability that any random male in USA would share the same genotype as the murderer (the match probability)? Assume that all 11 DNA samples analyzed in the diagram are together representative of the USA population as a whole. Show what numbers you would multiply to do this calculation.

The following figure shows the FBI-style analysis of the genomic DNA of 10 people (1-10), and also of hair found at a crime scence left by the murderer [***]. This analysis involves the PCR amplification of SSR loci, each from a different (nonhomologus) chromosome. The PCR primers are for each SSR locus are labeled with a unique fluorescent molecule. Some bands are thicker because relatively more of the corresponding PCR product was obtained. The figure has dots aligned on both sides to help you find the crucial bands; it will help to use a straight-edge as a guide. The numbers at right are the total number of copies of the SSR locus among the population of 11 samples. Are any of the loci on the Y chromosome? If so, identify this (these) locus (loci) by color.

Regarding STR markers used in forensic science. Tick all the correct statements: no correct statement the PCR primers used to amplify STRs are located in the repeat units PCR primers to amplify STRs are located on both sides of the repeat units the PCR primers used to amplify STRs are coupled to a fluorochrome which is essential for the detection of amplicons they are absent from the gonosomes the allelic frequencies of STR markers vary according to the ethnicity of the individuals genotyped they are present homogeneously throughout the nuclear genome

Please help asap

From your knowledge about DNA microarray, answer the following: A- How DNA microarray is created? and why it is referred to as “hybridization technology”?      B- Why RT-PCR is important in the sample preparation to perform expression microarray experiment?     C- Mention the name and the color of the dyes used in expression microarray?      D- If the expression microarray experiment was done with a normal sample and a suspected sample, after reading the color pattern resulted from the experiment it was recorded that “gene A22” is expressed in the suspected sample. The gene A22 is clinically linked to colon cancer. Answer the following: What is the expected color of the spot on the microarray which represents this gene? What is your interpretation of the suspected sample; is it a cancer sample or not and explain why?

From your knowledge about DNA microarray, answer the following: If the expression microarray experiment was done with a normal sample and a suspected sample, after reading the color pattern resulted from the experiment it was recorded that “gene A22” is expressed in the suspected sample. The gene A22 is clinically linked to colon cancer. Answer the following: What is the expected color of the spot on the microarray which represents this gene? What is your interpretation of the suspected sample; is it a cancer sample or not and explain why?

= Menu #2 Mol Bio Mutation QU... X + Create All tools Edit Convert E-Sign Q Search Complete the following tasks. Sign in Find text or tools Q H You are fresh recruits to a molecular biology laboratory, and your new boss has tasked you to study mutations in NRAS, a gene that she suspects is involved in cancer pathogenesis. Task A: Polymerase Chain Reaction Master Mix Your first task is to isolate and amplify the NRAS gene from cDNA extracted from different samples through PCR. You will need to run a total of 7 PCRs: 3 normal samples, 3 cancerous samples, and 1 negative control. To make things easier in the lab, when running multiple reactions, the components are prepared not individually, but as a master mix-all the components for multiple reactions are prepared in bulk, except for the template DNA, which is added separately once the master mix has been distributed into individual tubes. The table below lists the different components for PCR, the available stock concentrations of these…

My professor instructed me to make a PowerPoint presentation on this topic " Prenatal Molecular Testing ". I have total 4 members in my group.  Can you please divide the topic into 4 sub topic or parts?  So that we can individually take preparation. I will rate you positive if you do so.  Thank you so much. ?

My professor instructed me to make a power point presentation on this topic" Prenatal Molecular Testing ". I have total 4 members in  my group,  Now can you divide this topic into 4 sub topic or parts,  which we can work?  Please divide the topic into 4 parts,  i will rate you positive if you do so.  Thank you. ?

DNA fingerprinting questions: ( Please label each lane so I know which one you refer to)  Please explain vividly so I can understand:  •Explain each lane, by analyzing the band pattern and concluding whether it's homozygous or heterozygous for the presence of Alu at the PV92 locus. •Literature states Alu insertions at PV92 locus are most common in the Asian population (+/+) and mostly not found in Europeans, Americans (-/-) Reference: https://www.mediafire.com/file/s3nr36ow4bw4udw/DNA+Fingerprinting.docx/file

Select all that would be true if I had a nonsense mutation in an exon of a gene: The nonsense mutant allele would be the same size as wildtype by PCR-electrophoresis The nonsense mutant protein would be the same size by Western as the wildtype protein The nonsense mutant allele would be a different size compared to wildtype by PCR- electrophoresis The nonsense mutant protein would be a different size by Western compared to the wildtype protein

FOR PKU:  Draw what you’d expect to see if you were to analyze the gene products by conventional PCR, RT-PCR and Western blot. Would you see abnormally sized gene products?  Explain and be sure to include any relevant control(s) that you should include in your experiment.

Genome Wide Associated Studies:   Question 7 options:   use SNP arrays to statistically link single nucleotide polymorphisms to diseases   use Next Generation Sequencing technology to identify genetic variances within protein coding regions on DNA to identify genetic variances involved in diseases   use Sanger Sequencing to identify single nucleotide polymorphisms   use Next Generation Sequencing technology to identify single nucleotide polymorphisms within the entire genome   studies prespecified sub-regions of the genome to identify genetic variants statistically associated with diseases

Place the steps of whole genome sequencing in order.

Answer the following correctly. Designer Genes Work (This is all about Applications of Recombinant DNA). Illustrate Designer genes using this information: The Arctic apple is a fruit engineered to resist browning after being cut. Currently they are only available in the US – in golden, fuji and gala varieties – where they have been given Food and Drug Administration approval. If approved in Europe, they would have to be labelled as genetically modified. The manufacturers claim the main benefit is to help cut down on food waste. And based on the following: a. Identify a special trait. b. Identify a source organism. c. Identify a target organism d. Identify the modified/added trait. Example Answer: Hot Tomato > Chili > Tomato > Spicy Tomato It was reported this week that Brazilian scientists are hoping to create spicy tomatoes using Crispr gene-editing techniques. Although tomatoes contain the genes for capsaicinoids (the chemicals that give chillies their heat) they…

please solve this with step-by-step calculations and explanations.

How will you know if the bacterial cells were transformed during the lab? notes are attached below

STEP BY STEP Exaplanation would be appreciated so I can understand for upcomming exam. In humans, the COL1A1 locus codes for a collagen protein found in bone.  A recessive COLA1 allele believed to reduce bone density and increase risk of fractures differs from the wild type allele by the presence a GT in the first intron of the gene. This mutation can be easily screened by PCR, using COL1A1-specific primers followed restriction enzyme digest of the product with MscI. The normal allele is denoted as G and the recessive allele as T.  A recent study of 894 women found that 570 were GG, 291 were GT and 33 were TT.  (Assume for this exercise that these values reflect both male and female values for the sampled population. What are the frequencies of each allele? What are the frequencies of each genotype? c. Is this locus in Hardy-Weinberg equilibrium? Use the table below to evaluate the significance of any deviation from H-W expectations.

help...

Please help me with this question within an hour???? with proper explaination ASAP

3. Animation 12.1: This table shows results from allele-specific oligonucleotide hybridization analyses conducted on three different patients. The analyses all used the same probes constructed to test for the presence of a mutant gene. A plus sign (+) indicates hybridization occurred and a negative sign (-) indicates no hybridization occurred. Patient #1 Patient #2 Patient #3 Probe for normal allele Probe for mutant allele Which patient(s) is/are homozygous for the mutation and which patient(s) is/are heterozygous for the mutation? O Patients #2 and #3 are homozygous and Patient #1 is heterozygous for the mutation. O Patient #2 is homozygous and Patient #1 is heterozygous for the mutation. O Patient #1 is homozygous and Patients #2 and #3 are heterozygous for the mutation. O Patient # 1 is homozygous and Patient #2 is heterozygous for the mutation.

Please answer asap and in short and content should not be palgarised please

fueled.brightspace.com/d2l/le/enhancedSequenceViewer/3300467?url=https%3A%2F%2Ff59af8a9-95f5-419c-a486-a A rdschools.com bookmarks Drive Classes B Login Page Sign In Education and Lear... Content = 1.08 Unit Test: Gene Expression - Part 1 Which statement is most accurate? Hair is different from kidneys because the cells that make up hair and kidneys have different genes All cells have the same genes, but different genes are active in different cells. As cells and tissues differentiate, they produce new genes. All cells have the same genes, and all of a cell's genes are active at the same time. #m C $ J лае 1 2 3 4 5 & 7 8

TOPIC: MAKING A Recombinant DNA model

BOTTOM The sequence depicted on the gel is 5'-GTGATGTAG-3' The sequence depicted on the gel is 3'- GTGATGTAG -5' The sequence shown on the gel is the sequence of the template strand of the reaction The sequence shown on the gel is complementary to the template strand of the reaction The template strand of the reaction is antiparallel to the sequence shown on the gel The largest fragment shown on the gel is closest to the top The largest fragment shown on the gel is closest to the bottom Each of the fragments shown on the gel have a primer incorporated at their 5' end Each of the fragments shown on the gel have a primer incorporated at their 3' end The number of nucleotides in the shortest band on the gel is 21 The number of nucleotides in the longest band on the gel is 21 ick Save and Submit to save and submit. Cick Save All Answers to save all answers.

Transcriptome analysis involves two separate methodologies: gene expression and RNA seq analyses. The 10 items below are a scrambled listing of the steps used in the two procedures. Identify the steps involved in RNA seq from the list below. Use the numbers in the list to refer to each step. Once the steps for RNA seq have been identified, write the steps in the order in which they are performed during the experiment.   (1) DNA sequencing (2) Allow for hybridization and wash excess cRNA. (3) Mix labeled cRNA with array chip. (4) PCR amplification (5) Measure fluorescence intensity to determine abundance of transcripts. (6) Add labeled cRNA at each microarray location. (7) Map cDNA sequences to the genome of the organism to determine identity and abundance of transcripts. (8) mRNA isolation from cells (9) Prepare fluorescently labeled cRNA probes (10) cDNA synthesis

The technique of fluorescence in situ hybridization (FISH) is described. This is another method for examining sequence complexity within a genome. In this method, a DNA sequence, such as a particular gene sequence, can be detected within an intact chromosome by using a DNA probe that is complementary to the sequence.For example, let’s consider the β-globin gene, which isfound on human chromosome 11. A probe complementary to theβ-globin gene binds to that gene and shows up as a brightly colored spot on human chromosome 11. In this way, researchers can detectwhere the β-globin gene is located within a set of chromosomes. Becausethe β-globin gene is unique and because human cells are diploid(i.e., have two copies of each chromosome), a FISH experimentshows two bright spots per cell; the probe binds to each copy ofchromosome 11. What would you expect to see if you used thefollowing types of probes?A. A probe complementary to the Alu sequenceB. A probe complementary to a tandem array near…