Streak+Plate+Questions

Escherichia coli Mycobacterium phlei Bacillus Micrococcus subtilis luteus A B Figure 1: Agar Slant Cultures of Bacteria (Gary E. Kaiser, Ph.D.- Professor of Microbiology) . Observe and describe the colour of the slant cultures (A-D) in fig 1. ( . Define the following terms: pure culture, sterile medium, inoculum, aseptic technique, and colony. What is the name of the cultures that you used . Where they gram negative or positive Define the following terms: psychrophile, mesophile, thermophile, and hyperthermophile.

Using the attached image, explain the observed results on the NA and ECM plates. Be sure to address why each sample is either able or unable to grow on each plate and include the genotype(s) of the cells which are capable of growth.

The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution Plate 3 107 dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* 382 83 10 2 0

The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate 1 10 Plate 2 10 Plate 3 10 dilution dilution dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 10 dilution -6 *Too many to count Number of colony forming units (CFU) TMTC* TMTC* 840 28 19 1

Answer the following questions:   1. Define a bacterial colony.   2. What is the difference between macroscopic and microscopic appearance of bacteria?   3. State the three standard terms used to describe single colonies on agar plates.   4. State and define the three types of growth that may be seen in a broth culture.   5. State three basic shapes of bacteria.   6. State and describe the different arrangements of cocci.   7. What is the difference between true motility and Brownian motion?

You have identified two colony types A and B on the streak plate.  Now briefly describe (in 3-4 sentences) the process of how you identified the cellular morphology/arrangement of each isolate. Again, assume you have all the necessary equipment and materials at your disposal.  Be concise and thorough, not verbose; e.g., refer to the Gram stain, but describing the details of each step is not necessary. Following the description, provide the cell morphology information for each isolate.   Isolate A – Describe:    Cellular morphology & arrangement:    Gram stain color & result: Isolate B – Describe:    Cellular morphology & arrangement:    Gram stain color & result:

The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with warm molten agar and poured into a Petri dish. The numbers of bacterial colony forming units (CFU) after overnight incubation are shown in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution -5 Plate 3 10 dilution Plate 4 10 dilution Plate 5 107 dilution -8 Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* TMTC* TMTC* 867 154 18

First page is reference for number 4-7

Use the attached image to help explain the results on the NA and ECM plates. Be sure to address why each sample is either able or unable to grow on each plate and include the genotype(s) of the cells which are capable of growth.

Hii, can anyone help me with this. Your help would be very much appreciated, thank youuu.

Answer the attached photo below

Complete the table below with the observations: include amount of growth (-, +, ++, +, ++), colonial morphology for NA, color of colonies for EMB, and media color for MSA.   Organism/ Media Nutrient Agar (NA) Eosin Methylene Blue (EMB) Agar Mannitol Salt Agar (MSA) Escherichia coli       Pseudomonas aeruginosa       Staphylococcus epidermidis       Staphylococcus aureus       Unknown 9 mixture

Answer and the image below and provide explanations.

can you please help me with this? Thank you.

Answer completely and correctly: A pure culture of an unknown bacterium was streaked onto plates of a variety of media. You notice that the colonymorphology is strikingly different on plates of minimal media with glucose compared to that seen on trypticase soy agar plates. How can you explain these differences in colony morphology?

Need to submit within an hour please help

Good day, could you please help me answer the following. This is just 1 problem, the questions are connected. Thank you so much! a) A commercial stage micrometer has a total length of 1000 μm and is divide into 100 equal divisions. What is the length of each stage division in millimeters? b) Fourteen (14) ocular divisions coincide with three stage divisions. Assuming that the graduations of the stage micrometer are spaced 10 μm, what is the known distance between the three stage divisions in millimeters? c) With the same given information on b), what would be the distance of one ocular division?

describe the shape of the bacterial cells for each of the two yogurt bacteria. Be sure to use the terms for bacteria shapes that you learned in the Survey of the Microbial World lab!

Why are the results of plate counts expressed as the number of colony-forming units, rather than the number of cells?

Thank you so much

can you please help me to write short answer for these question 1.why it is important to complete morphological and cultural characterizations before pursing physiological testing? 2.In regard to taxonomic classifaction of bacteria, what is the relationship between physiological and genetic differentiation of bacteria?

Which section shows a growth phase where the number of cells dying equals the number of cells dividing,  a growth phase where the number of cells dying is greater than the number of cells dividing,  and a growth phase where the cells dividing at their maximum rate of division?

give the ABA DABA 3 letter combo for this bacteria

Complete the colony morphology for the colonies pictured above: 1.  Form - 2.  Elevation - 3.  Margin - 4.  Surface - 5.  Chromogenesis -

Below are morphological, physiological and biochemical characteristics of an unknown luminous bacterium isolated from the gills of a marine fish. Use the data below to identify the isolate. Make an identification scheme   Test Unknown Isolate B 1 Luminescence Positive 2 Cell Shape Straight Rods 3 Gram Staining Negative 4 Oxygen Requirement Facultative Anaerobe 5 Motility           (Hanging Drop) Motile 6 Growth at 0% NaCl Negative 7 3% NaCl Positive   8              6 % NaCl                                      Positive                   9 Accumulation of PHB Positive 10 Flagella 1 Polar 11 Oxidase Positive 12 Nitrate Reduction   13 Gelatinase Negative 14 Growth at 35OC Positive 15 Growth at 4OC Negative 16 Methyl Red Positive 17 Voges-Proskauer Positive 18 Catalase Positive 19 Starch Hydrolysis Negative 20 Lysine…

The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter   Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?

Please answer fast What is this organism? And what other test could be done to confirm it's identification?   1.   Gram stain - positive cocci, chains   Hemolysis- gamma   BE - positive   NaCl - positive   Catalase - weakly positive   2.   Gram positive cocci, chains   NaCl - negative   BE - negative   Hippurate - positive   Hemoylsis - beta   Catalase - negative

Answer the question in complete sentences and paragraphs

Help with my unknown lab report for staphylococcus aureus.

Purpose Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures you have learned about during the semester. Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes You must write up your OWN dichotomous key for all the possible unknown organisms listed on above. Writing this key requires you use the same type of reasoning used in the Dichotomous key lab. The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Please help me with this question. Thank you so much !