6_Proj

Lesson 2 Focus Questions 1. What chemicals and molecules are needed for PCR, and what is the function of each component? 2. Examine the 150 base promoter sequence below. Kaylee Kauff 5'TAGAAAAGGA AGGTGGCTCC TACAAATGCC ATCATTGCGA TAAAGGAAAG GTATCATTC AAGATGCCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC ACCCACGAGG AGC ATCGTGG AAAAAGAAGA CGTTCCAACC ACGTCTTCAA3' Write in the sequence of the complementary strand and mark the 3' and 5' ends of the complementary strand. 43

Task 1: Compare the characteristics of DNA to RNA. Think before you drop - This is an all or nothing type of question. Double check that you are happy with where you placed all of the options before you submit the knowledge check. DNA RNA No Answers Chosen No Answers Chosen DNA & RNA No Answers Chosen Possible answers Phosphate group | Cytosine Adenine Basic unit is the nucleotide Single strand Double helix E Phosphate-sugar backbone | Uracil | Guanine | Thymine :::: ::::

Task A: Polymerase Chain Reaction Master Mix Your first task is to isolate and amplify the NRAS gene from cDNA extracted from different samples through PCR. You will need to run a total of 7 PCRs: 3 normal samples, 3 cancerous samples, and 1 negative control. To make things easier in the lab, when running multiple reactions, the components are prepared not individually, but as a master mix—all the components for multiple reactions are prepared in bulk, except for the template DNA, which is added separately once the master mix has been distributed into individual tubes. The table below lists the different components for PCR, the available stock concentrations of these components, and the needed working concentrations for the PCR itself. Complete the table by supplying the needed volumes of each component for a single reaction and for the master mix (the first row has been filled in as an example).

I need help writting a biological "title" for this lab report. I performed 5 labs which are PCR purification, Rapid ligation, and Transformation, Blue/White screening and observation of pGreen transformation, Plasmid Extraction and Restriction Enzyme Digestion, Soil DNA extraction and gel electrophoresis, and Bioinformatics Analysis of 16s rRNA genes.   Note; I attached one page of my abstract and one page of the introduction

Legrning Task 2: Make a.model of a DNA template to deternmine the seguence of bases in th new DNA strahds Then, answer the gulde questions that follow. Materials: crayons. Scissors, paste/tape, used folder or illustration board Procedure: Use the pattern of the DNA templater (attached to this LP). Color code, phosphate = blue, deoxyribose sugar = green, nitrogenous base as follows: adenine= yellow, thymine = pink, guanine = violet, cytosine = red. And cut the shapes of each nucleotides. Buld a model of a strand of a DNA molecule. The strand should contain 6 base" rungs" following the given order of the nucleotides: Guanine, Adenine, Cytosine, Thymine, Cytosine, Guanine. Tape the cutout pattern to form the nucieotides. This will represent the left half of DNA. Make a complementary strand that you made in step 3. Tape the cut -out pattern again forming the nucieotides for the second strand of the DNA molecules. Match the bases of the first strand and the second strand. Do not tape across…

Instructions: Read 13-2 Manipulating DNA pages 322-323. As you read each section, examine the figures and captions (explanations). Identify any questions you may have. 1) Develop an analogy for the processes researchers use to make changes to DNA. In yo analogy, explain how it is similar to the techniques used in genetic engineering. You can draw a graphic organizer, make a table, or write a few sentences describing your analogy. 2) Devise flowchart that shows the steps to prepare DNA for gel electrophoresis, as well the protocol for setting up and running a gel. You can add diagrams to the flowchart an add detailed notes if you like. English (inited Sate) O Focs ere to search 4 CO RU G\ L B. 2N A\ Alt Ciri

WORKSHEET TASK 3: 1.     Below is a theoretical section of DNA. Design two primers that are 10 base pairs (bp) long that will amplify this section of DNA in a PCR reaction (‘N’ refers to non-specific ‘nucleotide’).     3’–A C G T G A A C T G C C T NNN......NNN C C G T G T A T C T C T T–5’   5’–T G C A C T T G A C G G A NNN......NNN G G C A C A T A G A G A A–3’

Task 3: Agarose Gel Electrophoresis Your amplicon from PCR was subjected to AGE for analysis. You are shown the image of the gel loaded with the following lanes: (A) negative control, (B) size ladder (1, 2, 3, 4, 6, and 10 kb), (C) gDNA extract, (D) PCR amplicon. However, due to mishaps while loading the gel with the samples, you are not sure which lane is which. You are shown a diagram of the obtained gel below.   a. b. Label each lane of the gel. Write only the corresponding letters in the wells above. Above each band in the size ladder, write its size (in kb). c.corresponding to the gene. Approximate the size (in kb) of the polystyrenase gene. Write your answer above the band Bonus: If you wish to identify the bacterial species in this scenario, what gene is most commonly and routinely sequenced?Answer: __________________________________

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BIOTECHNOLGY Date: Name: Instructor: Section/Group:. POST-LAB QUESTIONS 1. In one or two sentences, summarize the technique of gel electrophoresis. 2. How does the process of gel electrophoresis separate DNA fragments? 3. Why is the fact that DNA has a negative charge so important in the gel electrophoresis process? Biotechnology 165

Please help with questions 2 & 3, thank you!

Please help with this set up! I have tried for a couple hours and I am not getting anywhere. My calculations dont make any sense.

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Resriction Enzyme Worksheet #1

Please I want the answer of (B) and (C) Task 1. Your graduate advisor asks you to amplify the following sequence of DNA by PCR:   5’-ATACGCATTCGGACCAGGTCCTAA-3’   3’-TATGCGTAAGCCTGGTCCAGGATT-5’   a. To ensure that the entire sequence above is amplified, what 6-nucleotide DNA primers   should you add to your PCR mix?       You order the primers listed above, but instead receive the following set of primers:   5’-CGCATT-3’   5’-GGACCT-3’   b. What portion of the double stranded DNA molecule will be amplified after 10 rounds of   PCR?       Your labmate attempts to rescue your PCR reaction by providing you with the following   set of primers:   5’-ATACGC-3’   5’-TCCTAA-3’   c. What is the result of running the PCR reaction with your labmate’s primers? How many   double stranded molecules of DNA will result from 10 rounds of amplification?

Answer: Task 2: Polymerase Chain Reaction After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer briefly but completely. DNA polymerase isolated from Thermus aquaticus Answer: a. b. Deoxynucleotide triphosphates (dNTPs) Answer: Forward and reverse primers Answer: с. Task 3: Agarose Gel Electrophoresis Your amplicon from PCR was subjected to AGE for analysis. You are shown the image of the gel loaded with the following lanes: (A) negative control, (B) size ladder (1, 2, 3, 4, 6, and 10 kb), (C) GDNA extract, (D) PCR amplicon. However, due to mishaps while loading the gel with the samples, you are not sure which lane is which. You are shown a diagram of the obtained gel below. a. Label each lane of the gel. Write only the corresponding letters in the wells above. b. Above each band in the size ladder, write its size (in kb). c.…

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please help with both questions

Submit Q4.6. What does it mean to say that extension by DNA polymerase Ill proceeds 5' 3'? The 5' end of a DNA polymerase molecule attaches to the 3' end of primase. DNA polymerase adds nucleotides to a growing strand, moving in the 5'-→3' direction. DNA polymerase seals nicks as it moves along a DNA strand toward the 3' end. DNA polymerase can only synthesize DNA at the 5' end of an existing strand of DNA. Submit Q4.7. In DNA replication, what is the difference between thelleading and lagging strands? In the leading strand, DNA is synthesized 5'-3', while in the lagging strand it is synthesized 3'-5'. The leading strand is composed of DNA only, while the lagging strand is composed of both RNA and DNA. After extension, the leading strand is continuous, while the lagging strand is composed of disconnected fragr The leading strand is synthesized only by DNA polymerase II, while the lagging strand is synthesized only b Submit i DUO in thn nrocess of being replicated. The RNA primer is sho

I want to know how can I make RNA transcript through next dna sequencing. 5'ATGATCTTTAAAGGGCCC 3'

Learning Task 2: Make a model of a DNA template to determine the sequence of bases in th new DNA strands. Then, answer the guide questions that follow. Materials: crayons. Scissors, paste/tape, used folder or illustration board Procedure: Use the pattern of the DNA template: (attached to this LP). Color code, phosphate - blue, deoxyribose sugar = green, nitrogenous base as follows: adenine= yellow, thymine pink, guanine = violet, cytosine = red. And cut the shapes of each nucleotides. Build a model of a strand of a DNA molecule. The strand should contain 6 base" rungs" following the given order of the nucleotides: Guanine, Adenine, Cytosine, Thymine, Cytosine, Guanine. %3D %3D Tape the cutout pattern to form the nucieotides. This will represent the left half of DNA. Make a complementary strand that you made in step 3. Tape the cut -out pattern again forming the nucieotides for the second strand of the DNA molecules. Match the bases of the first strand and the second strand. Do not tape…

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Need help on question 3, please. The drop-down answer choices are in the image attached below.

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Please help me with this question within an hour need to submit an assignment please?????

please help me with thi question. What advantages do CRISPR‑Cas systems have over restriction enzymes and engineered nucleases for editing DNA? The options are attached. Multiple answers can be chosen

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Problem 4:  You are planning an experiment to use zinc finger nucleases to generate a double-stranded DNA break in a targeted sequence of DNA Name the protein domain in a zinc finger nuclease that is responsible for generating the DNA break Fok1 Name two other genome-engineering proteins that could be used for generating the double-stranded DNA break   What are the advantages and disadvantages of each strategy?

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Question:- Is DNA sequencing a in vivo, in vitro and/or in silico?   What product(s) is/are formed in DNA sequencing and how and where would the reaction begin? Also, what raw materials are needed?

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