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Lab 4 Selective Media & Agar BIO250L
”
Student Name:
Brandon Estelle Access Code (located on the lid of your lab kit):
ac-gif4su Lab Report Format Expecta0ons
U"lize college level grammar and professional forma4ng when comple"ng this worksheet. Submissions without proper forma4ng, all required photos or sufficient responses will be rejected. Pre-lab Ques>ons 1.
In this lab, you will conduct three different experiments that each cover the topic of selecAve media and agar. How are these experiments different from one another? Explain how the different media in each experiment are uAlized in the context of selecAve vs differenAal media. Click here to enter text. 2.
What is the difference between chemically defined and chemically complex media? Give an example (a clinical or environmental research applicaAon) of when you would use each type of medium to culture different microorganisms. Chemically defined media is known chemical composiAon while chemically complex contains unknown chemical composiAon. 3.
Why do we grow microorganisms on general growth media first, before inoculaAng differenAal media? To have a pure isolated colony that is able to grow without a medium affecAng growth, 4.
What changes might you see on a differenAal plate that helps idenAfy the bacteria? Gram staining
Lab 4 Selective Media & Agar BIO250L
”
EXPERIMENT 1: BIOPROSPECTING FOR STARCH DEGRADING BACTERIA Introductory Ques>ons
1.
What results do you expect to see in Experiment 1 if your selected colony is amylase-producing? Small round spots of bacteria growth 2.
What are some other potenAal sources of starch-degrading bacteria? Food, paper, soil, fermentaAon 3.
Why did you perform Step 10, under the Prepare Stock SoluAon secAon, in this experiment? To weigh porAons by measuring appropriate volume 4.
What kind of media did you use iniAally, and why? Starch agar because it contains bacteria that digests starches
Lab 4 Selective Media & Agar BIO250L
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Data and Observa>ons
In the table below, report your observed growth corresponding to each plate. Denote whether there was growth on the starch plate, whether or not clear areas formed around the colonies that grew. Table 1: Starch Agar and Gram Iodine Results Sample
Growth on Starch Agar? (Yes/N)
Clear area around colonies? (Y/N)
A
Yes
No
B
Yes
No
C
Yes
Yes
D
Yes
Yes
Lab 4 Selective Media & Agar BIO250L
”
Provide a clear, high resoluAon photo of your plates aWer incubaAon with the lids removed. For credit on this lab, your photo must show the growth that is reported in your tabulated data, must depict the correct media with the iodine added and must also include your handwriXen name in the background. Plate A
Plate B
Plate C
Plate D
Lab 4 Selective Media & Agar BIO250L
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Radiation Safety
Instructions
While working in the radiographic imaging field, you will be faced with the risks of excess radiation exposure. These risks are not insignificant,
and can potentially lead to a variety of health issues, Following the best radiation safety practices in the field will be beneficial both for patients
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