Lab 12 Assignment

9 and 10 please

General Electrophoresis Questions: 1. What makes macromolecules move through the gel in electrophoresis?2. What determines the speed at which macromolecules move through the gel in electrophoresis? In a single gel, why do some move faster than others?3. Why do we use different procedures for DNA and protein electrophoresis?

Helping tags: Biology, bacteria, lag phase WILL UPVOTE, just pls help me answer the following questions and explain them clearly. Thank you. TOPIC: Bacterial Growth Curve 1. How may the following growth and culture conditions affect the length of the lag phase? EXPLAIN YOUR ANSWERS. a) inoculum is from an old culture b) shifting cells from rich culture medium to a poorer one c) exponentially growing culture is transferred into the same medium under the same growth conditions

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Topic: Isolation of E. coli bacteriophage   What would happen if: - the enrichment method in the isolation of bacteriophage was omitted? - the chloroform was not added to the enrichment? - the 0.1 ml lysate-E. coli mix was plated directly on top of the bottom agar?

please solve this step by step calculations

BONUS (15 points) The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2. étv A) F12 F9 F10 F7 % & %3D delete 5 { T Y J K

Activity 13 Urine Culture Inoculating Urine with a calibrated loop   The number of microorganisms per milliliter recovered on urine culture can aid in the differential diagnosis of urinary tract infection (UTI). Plastic or wire inoculating loop available commercially, have been calibrated to deliver a known volume of liquid when handled correctly, thus enabling the microbiologist to estimate numbers of organism in the original specimen based on colony forming unit (CFU) of growth on cultures.   PROCEDURE: 1st day 1. Gently swirl the specimen bottle to mix the urine specimen. 2. Label all plated media with name of patients, clinical specimen used and date. Label at the bottom of plates and not on the cover. 3. Obtain a disposable calibrated loop. 4. Dip the loop straight into the urine specimen so that the loop part is completely covered. Withdraw straight out. See fig. 16-18 5. Inoculate blood agar plate (BAP) as shown in fig.16-19 6. Incubate at 35 – 37˚C for 18 – 24 hours. 7. Dispose…

10, 11 please answer all give the significance/role/effect of the reagent/condition in the isolation or analysis of a biomolecule. limit answers to two short sentences

ull MTN-Stay Safe LTE 10:13 AM @ 34% #3 Mol Bio Gene o... Complete the following tasks. You discovered that a species of bacteria can break down Styrofoam™ (polystyrene) products due to an enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme. Task 1: DNA Extraction To begin work on the bacterium, you begin by extracting its genomic DNA (gDNA). What is the purpose of the following procedures? Answer briefly but completely. Using sodium dodecyl sulfate, a detergent Answer: а. b. Adding RNase A and Proteinase K during extraction Answer: c. Adding ethanol before recovering the DNA extract Answer: Task 2: Polymerase Chain Reaction After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR using the appropriate gene-targeted primers. What is the purpose of the following PCR compon briefly but completely. Answer DNA polymerase isolated from Thermus aquaticus а. Answer: b. Deoxynucleotide triphosphates (DNTPS)…

Biomedical Engincering Dept. Biocompatibility Hello sir, I know it takes precious Lab. Sheet-Level time. Please, I have a report on this Semester 1 subject. Only 5 papers within a Experiment Title: Cytotoxicity Test discussion solution. I want to solve the text of the keyboard. Thank you, Experiment no. 2 sir 1 12:34 PMA Objectives: Study the eytotoxicity and how it is measured. 1- Background Cytotoxicity can lead healthy living cells to three potential cellular fates. 1. Necrosis (accidental cell death): Rapid loss of membrane integrity and cell lysis 2. Apoptosis (programmed cell death): slower, more orderly, and genetically controlled 3. Cytostasis (a decrease in cell viability): cells remain alive but fail to actively grow and divide Importance of measuring cytotoxicity two major reasons 1. Either you want specific cells to die and look for an adequate compound/condition (cancer and immunotherapy) 2. Or you want to exclude cytoloxicity in specific cells (chemicals and drugs)…

what are the volume dilution and dilution factor for bacteriophage A，B，C

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16. Your Excel data graph for Serial Dilution lab:** 16a. Include a clear and labeled graph of DF (x axis) versus Absorbance (y axis). See box above for expectations and review video tutorial of this Excel work. Do not graph two of your data points: your blank or your Cuvette #1 (DF=1). 1 Q A Multiple Styles W S X 3 E D $ 4 To C R F % 5 I T V U ^ 6 G Y 7 H U 8 J BN S ( 9 K M X

Pipetting 1. Explain why a micropipettor is an important instrument in biochemistry labs. 2. Describe the basic components (and function) of a micropipettor. Bacterial Techniques 1. Define the following. (a) Serial Dilution (b) Streak Plating (c) Spread Plating Transformation 1. Define bacterial transformation and explain why it is an important method in biochemistry labs. 2. Describe (figure, narrative) a plasmid and describe the basic components of a plasmid. 3. What role does CaCl2 play in bacterial transformation? 4. What role does heat shock play in bacterial transformation? Plasmid Isolation 1. Describe the roles the following play in plasmid purification. (a) Lysis Buffer (b) Neutralization Buffer (c) Elution Buffer

what is the most efficient way to solve this dilution word problem?

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question: Can you summarize and explain for me what you want to tell in the article below? When I read it myself, I do not understand exactly what is meant by the article. It would be nice if you could highlight the important points. You can use them in a figure or diagram to explain. thank you and hava a nice day :) Article: Nanomaterial-Based Vaccine Development and Immunomodulation Following the publication of the genetic sequence of SARS-CoV-2 on January 11, 2020, intense research efforts have been devoted to developing a vaccine against COVID-19. With unprecedented speed, this extraordinary scientific mobilization led the first vaccine candidate to enter the Phase I human clinical trial on March 16, 2020, and other novel candidates are rapidly following. Up to May 22, 2020, there are 10 COVID-19 candidate vaccines in clinical evaluations and 114 in preclinical development.  Concerning vaccine and immunization research, nanomaterials can assist in multiple ways to boost the…

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Discusses the methods to -1 optimise fluorescent cellular (staining (priority first List the freezing -2 parameters of the slow cooling technique Parameter of verification -3 precedure of freezing 9

Please written by computer source 1. We want two T-75 flasks, each with 200,000 cells in the flask that holds 20 mL of culture medium per flask. Hemocytometer counting of cells in a 1 mm sq2 area gave an average of 12 cells from a 1:9 dilution of the cell suspension.   a.) Determine the cells/mL. b.) What is the volume of your cell suspension needed to make the flasks? c.) How you would now make the flasks as a new subculture of cells for incubation?

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General Electrophoresis Questions: 1. Compare and contrast the sample buffers for DNA and protein electrophoresis. 2. Compare and contrast the running buffers for DNA and protein electrophoresis.

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Question:- Two different patients suspected of having a urinary tract infection submitted fresh urine samples to a lab for culture and antibiotic sensitivity testing. The samples were inadvertently left out overnight at room temperature. The next day, a medical technologist discovered the samples. Upon opening the first sample, the strong smell of ammonia was noticed. The second urine sample did not have a strong ammonia smell. Based on these observations, which urine sample most likely contained cells of the bacterium Proteus Vulgaris? Explain your answer. If these two urine samples had not been noticed until a week later, would you expect there to be a difference in the “odor” of the samples? Explain your answer.

Please help with all parts of this problem. Double check your answers.

Helping tags: Biology, bacterial count, dilution, serial dilution WILL UPVOTE, just pls help me answer the following questions and explain them clearly. Pls show complete solutions also for the computation part. Thank you. 1. A bacterial culture was grown for 9 hours. At 3-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.85% NaCl. Three consecutive dilutions were further made by using 1 ml aliquot in 9 ml of 0.85% NaCI. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. Sampling COUNTS 2nd dilution 3rd dilution 4th dilution 0,0 30: 35 250;245 1st dilution 1st 2nd 3rd 0; 0 240; 235 TNTC 55; 60 5; 6 TNTC TNCT TNTC TNTC a) Illustrate the dilution series used and label the final dilution of each dilution. b) Determine the bacterial count (CFU/ml) every 3 hours of incubation for 9 hours. Show all computations.

Average plaques for bacteriophage A，B，C are 137，36，25. PFU/ml is average plaques multiply by the volume dilution multiply by the dilution factor. Show your working for each bacteriophage.

AE, 62, Male Wt: 75 kg, Ht: 150 cm Orders: Penicillin G Potassium            10,000,000 Units Sterile water for injection                              qs Make a 250,000 U/mL solution.   The package insert states If Penicillin G Potassium 10,000,000 Units is reconstituted with 44 mL sterile water, a solution of 200,000 U per mL will be obtained.   What volume of sterile water would you use to reconstitute the penicillin G potassium in order to make the ordered solution?

BIOTECHNOLGY Date: Name: Instructor: Section/Group:. POST-LAB QUESTIONS 1. In one or two sentences, summarize the technique of gel electrophoresis. 2. How does the process of gel electrophoresis separate DNA fragments? 3. Why is the fact that DNA has a negative charge so important in the gel electrophoresis process? Biotechnology 165