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וןווד ← Q Lab Report 6 worksheets 314 F22 .DOCX File Edit View Insert Format Tools Help A 100% ¿ Summary Grades for Arysta Visser: 23 x M Uh-oh! There's a problem w X b The restriction EcoRI cleaves X + Untitled spreadsheet - Goog X https://docs.google.com/document/d/1mKY1HIgMPRh1kRDCmX7msBF2yf07-ogT/edit Outline Headings you add to the document will appear here. Normal text Times ... 12 + B I U 2 18. The restriction EcoRI cleaves double-stranded DNA at the sequence 5'-GAATTC-3', the restriction enzyme HindIII cleaves at the sequence 5'-AAGCTT-3', and the restriction enzyme BamHI cleaves at 5'GGATCC-3. An 805 bp circular plasmid is digested with each enzyme individually and then in combination, and the resulting fragment sizes are determined by means of electrophoresis. The results are as follows: 1 Restriction Enzyme(s) EcoRI BamHI HindIII EcoRI and BamHI EcoRI and HindIII BamHI and HindIII 3 Practice ====•=•€ EX Fragment lengths (base pairs) 430 bp, 375 bp 470 bp, 335 bp Lab Report 6…

Is Table Window Help 1 Exam_2_problem_set_new- Saved to my Mac Design Layout References Mailings Review View AaBbCcDdF AaBbCcDdF AaBbCcDd AaB Normal No Spacing Heading 1 Не s, fixes, and improvements, choose Check for Updates. 2. Calculate the effective dilution. You were given a sample which contained 5.5mL of bacterial culture. You added 10uL of phage solution to the culture. What was the effective dilution produced by the addition of the phage solution?

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Is the inhibitor competitive uncompetitive or non competitive? Why?

Gmail .ll LTE 9:31 PM 40% #3 Mol Bio Gene o... Q •.. Complete the following tasks. You discovered that a species of bacteria can break down Styrofoam™ (polystyrene) products due to an enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme. Task 1: DNA Extraction To begin work on the bacterium, you begin by extracting its genomic DNA (gDNA). What is the purpose of the following procedures? Answer briefly but completely. Using sodium dodecyl sulfate, a detergent Answer: а. b. Adding RNase A and Proteinase K during extraction Answer: Adding ethanol before recovering the DNA extract с. Answer: Task 2: Polymerase Chain Reaction After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer briefly but completely. DNA polymerase isolated from Thermus aquaticus a. Answer: b. Deoxynucleotide triphosphates (DNTPS)…

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BIOTECHNOLGY Date: Name: Instructor: Section/Group:. POST-LAB QUESTIONS 1. In one or two sentences, summarize the technique of gel electrophoresis. 2. How does the process of gel electrophoresis separate DNA fragments? 3. Why is the fact that DNA has a negative charge so important in the gel electrophoresis process? Biotechnology 165

Helping tags: Biology, bacterial count, dilution, serial dilution WILL UPVOTE, just pls help me answer the following questions and explain them clearly. Pls show complete solutions also for the computation part. Thank you. 1. A bacterial culture was grown for 9 hours. At 3-hour interval, the culture was sampled to determine the population of the culture, by transferring 25 ml of the suspension to 225 ml 0.85% NaCl. Three consecutive dilutions were further made by using 1 ml aliquot in 9 ml of 0.85% NaCI. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. Sampling COUNTS 2nd dilution 3rd dilution 4th dilution 0,0 30: 35 250;245 1st dilution 1st 2nd 3rd 0; 0 240; 235 TNTC 55; 60 5; 6 TNTC TNCT TNTC TNTC a) Illustrate the dilution series used and label the final dilution of each dilution. b) Determine the bacterial count (CFU/ml) every 3 hours of incubation for 9 hours. Show all computations.

Assignment_12.pdf - Adobe Reader File Edit View Window Help Оpen 1 / 1 99,1% Tools Fill & Sign Comment 3. Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell growth. The recombinant protein can be considered a product of cell culture even though it is not secreted from the cells; it is synthesized in addition to normal E. coli biomass. Ammonia is used as the nitrogen source for aerobic respiration of glucose. The recombinant protein has an overall formula of CH1.5500.31N..25. The yield of biomass (excluding recombinant protein) from glucose is measured as o.48 g/g; the yield of recombinant protein from glucose is about 20% of that for cells. How much ammonia is required? What is the oxygen demand? If the biomass yield remains at 0.48 g /g, how much different are the ammonia and oxygen requirements for wild-type E. coli that is unable to synthesize recombinant protein? а. b. с. 24°C 08:39 Berawan 27/04/2022

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helping tags: biochemistry, agarose gel electrophoresis, genomic DNA isolation, gel electrophoresis Please explain your answers thoroughly. Thanks will upvote.

I BIO-103-LAB-WORKSHEET-3_BACTERIAL_MORPHOLOGY.docx - Trio Office Writer File Edit View Insert Format Styles Table Form Tools Window Help Abç # a a a ab ah & a 三、三军三E Default Style Calibri 12 I 1 I II Guide Questions: Illustrate the different forms of bacteria. 1) Spherical - coccus diplococcus streptococcus staphylococcus mono coccus 2) Rod-shaped bacillus club-shaped bacilli rodswith square ends fusiform bacilli rods with rounded ends 3) Curved rod - spir illa a. rigid form Spirillum vibrio Page 2 of 3 143 words, 1,416 characters Default Style English (USA) + 80% 8:26 AM P Type here to search 13 15 2/6/2021

Name: Chemotaxis Lab Worksheet 1. What is a model organism and why is it important to scientific studies? List two common model organisms, one for prokaryotic studies and one for eukaryotic studies.

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Need help 1. Explain how to isolate ribosome from liver tissue. 2. What kind of technique that can help to isolate 23S ribosomal RNA from RNA mixture

TOPIC: E. coli T4 (T4 Bacteriophage) Include a maximum of 3 sentences, a brief description of their growth. What are the structures responsible for growth and reproduction? What are their physical and nutritional (or chemical) requirements?

question: Can you summarize and explain for me what you want to tell in the article below? When I read it myself, I do not understand exactly what is meant by the article. It would be nice if you could highlight the important points. You can use them in a figure or diagram to explain. thank you and hava a nice day :) Article: Biomimetic Engineering of Nanodelivery Systems: Artificial Viruses in the Making In an effort to engineer the next generation of nanoscale vectors, scientists have moved from using inorganic components aimed at obtaining inert structures to utilizing biological building blocks that are able to convey additional functionalities to the resulting construct. To cope with the complexity of the body and to evade the multiple layers of defense that tissues and organs have, it is critical to rely on the ability of certain materials to interact with, rather than to eschew, the biology of our body. Every NP system conceived to date faces one common fate: whether injected,…

Helping tags: Biology, bacteria, lag phase WILL UPVOTE, just pls help me answer the following questions and explain them clearly. Thank you. TOPIC: Bacterial Growth Curve 1. How may the following growth and culture conditions affect the length of the lag phase? EXPLAIN YOUR ANSWERS. a) inoculum is from an old culture b) shifting cells from rich culture medium to a poorer one c) exponentially growing culture is transferred into the same medium under the same growth conditions

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Lesson 2 Focus Questions 1. What chemicals and molecules are needed for PCR, and what is the function of each component? 2. Examine the 150 base promoter sequence below. Kaylee Kauff 5'TAGAAAAGGA AGGTGGCTCC TACAAATGCC ATCATTGCGA TAAAGGAAAG GTATCATTC AAGATGCCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC ACCCACGAGG AGC ATCGTGG AAAAAGAAGA CGTTCCAACC ACGTCTTCAA3' Write in the sequence of the complementary strand and mark the 3' and 5' ends of the complementary strand. 43

Lab Dilution problem you start with a bacterial concentration of 10,000,000 bacteria/ml Construct a dillution scheme with 5 dillutions so that you plate out 100 bacteria. Please do step by step simplified. Explain the math its been a while.  Try to write it out and not do scientific notation please. Thank You

Helping tags: Biology, gram staining, archaea Can gram staining be employed as a taxonomic marker for members of Archaea? Explain your answer and cite examples to support your answers if necessary. *** Will UPVOTE, just pls help me answer the question. Thank you!

Module 10 DNA & Protein - 20 O Module 9 Genetics - 2021SP/B X B Take Test: Heredity 2 Homewor X + A sunyocc.open.suny.edu/webapps/assessment/take/launch.jsp?course_assessment_id=_109843_1&course_id=_53817_1&content_id3D_ * Question Completion Status: QUESTION 1 Some dogs bark while following a scent trail; others are silent. The barker allele is dominant (B) to the silent recessive allele (b). The allele for straight tails is dominant (S) over the allele for curly tails (s). If two dogs are heterozygous for both traits, which of these correctly identifies the genotypes of the parents? O a. BbSs x BbSs O b. BbSs x bbss O c. BbSs x bbSs O d. Bbss x bbSs QUESTION 2 Some dogs bark while following a scent trail; others are silent. The barker allele is dominant (B) to the silent recessive allele (b). The allele for straight tails is dominant (S) over the allele for curly tails (s). If two dogs are heterozygous for both traits, when we "FOIL" to see what allele combinations they can give to…

Mailings Review View Help es in viruses. Unless you need to edit, it's safer to stay in Protected View. Enable Editing 6. If the y-intercept of a Lineweaver-Burk plot = 1.91 (sec/millimole) and the slope = 75.3 L/sec, KM equals: a 0.0254 millimolar (mM). b. 0.523 millimolar (mM). c. 5.23 millimolar (mM). d. 39.4 millimolar (mM).

References Review View Help RCM Tell me what you want to do Editing v 12 A A BI U Av ... 2. Use the above photo to answer the following questions: a. What organism did you observe these cells in: b. What stain was used and what is it binding to causing this dark Header color: (stain) and bound to) c. What are the circles called in the cell staining dark: 100% lish (US) Text Predictions On A D home insert delete end prt sc 11 F12 F8 F9 8.

helping tags: biochemistry, PCR, polymerase chain reaction, PCR cocktail preparation Will upvote, just please help me solve the answers for the mastermix. Please show solutions. Thanks.

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Give a lab report introduction. introduction should include a general background on bacteriophages, the use of bacteriophages in bacterial genome engineering, and a description of the overall practical aims. Total word count should be no greater than 500 words.