Lesson11 Actin-based motility_WORKSHEETS
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MCB*4580 Membrane Biochemistry
Lesson 11 Cytoskeleton III –Actin-based motility
Instructions:
Answer the following questions using any resources
you can obtain. When you are done, submit your answers as a Word document to the Dropbox folder
.
Contributions:
If you worked in a group, list the members of the group and their contribution(s) to these responses:
Paper #1: Loisel et al 1999
What are the three main points
of the paper? (Final paragraph Paper) List the basic components (buffers and proteins) and their concentrations in the motility medium that the authors observed Listeria
motility (Paper). Drawing on your own experience and other resources, very briefly note what each component in the solution does
(your experience + internet). The motility medium contained: What was the maximum motility rate
for Listeria
in the motility assay (Paper)? Paper #2: Ohkawa et al 2010
In Figure 1A bottom time series pictures— What did they do to the baculoviruses and the cells so they could visualize
the viruses and actin in the cells? (Methods ) What kind of cells
are these (Methods)? What organism do they come from? (Internet) What evidence from Figure 1
suggests that the motility of the viruses is due to the P78/83 protein? What evidence confirms the involvement of actin polymerization and Arp 2/3 proteins in the motility of baculoviruses? Compare the velocity
of baculoviruses to those of Listeria
or Shigella
bacteria inside living cells (Paper). THINKING QUESTION
MCB*4580 Membrane Biochemistry
You want to test the idea that the P78/83 protein from baculovirus is the only
protein required for motility of the virus in an in vitro
motility assay. What kind of object would you track and how would you ensure that you are only testing the P protein, and nothing else from baculoviruses?
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Related Questions
Course: SCI Biology
Unit: Unit 08: Cell Membrane
SCI Biology - Unit 08: Cell Mer
1) The process of moving a large substance into a cell by means of a vesicle.
OA.) Endocytosis
OB.) Exocytosis
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2) The process of moving a large substance out of a cell by means of a vesicle.
OA.) Exocytosis
R) Endorytosis
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100% water
Part D: Application
4% water
1. The paramecium (see Figure 3-1) is a single-celled organism. Go to the
web and look up this organism. Now select an answer from each blank.
H2O
(iso-, hypo-, or
nucleus
oral groove
Paramecia live in a(n)
hypertonic) environment. In this environment, the paramecium takes
in a lot of water through the process of
(osmosis, diffusion, facilitated diffusion, or active transport). In order
to get rid of this excess water, the paramecium has a specialized
organelle called the contractile vacuole that acts as a water pump
-contractile vacuole
to expel water through the process of
(osmosis, diffusion, facilitated diffusion, or active transport).
FIGURE 3-1 Paramecium.
30
Anatomy and Physiology | Laboratory Manual
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12
Time Left:0:04:09
Angelina Tran: Attempt 1
O anabolism
Question 50 (2 points)
Listen
Which of the following is NOT part of the endomembrane system?
O chloroplast
plasma membrane
vesicles
endoplasmic reticulum
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Concentration, Osmosis, and Cell Environments HW Quiz
For each of the drawings, label what kind of environment it is in, how you know this, and what is happening to the cell.
This cell is in a isotonic
v solution.
I know this because
This cell will
More water is coming in than going out
More water is going out than coming in
The same amount of water is going out and coming in
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Discuss the following:
• Golgi Apparatus
• Peroxisome
•lysosome
Please help me. Thank you
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Cell Membrane and Cell Transport WebQuest
Part I: Cell Membranes
Go to the following website: www.biology4kids.com/ files/cell_membrane.html
1. How is the cell membrane similar to a plastic bag with tiny holes?
2. What two components make up the cell membrane?
a. What are their functions?
3. What is the fluid mosaic model?
4. Sketch a section of the cell membrane, showing both phospholipids and proteins.
Label your drawing.
5. Label the diagram of the phospholipid molecule below with the following terms:
hydrophilic head, hydrophobic tail
This diagram
shows a
Head
simplified
version of a
phospholipid
molecule.
Tails
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i testnavcient.psonsvc.net//question/33ele03d-57b0-42eb-9848-cB0c304bla19/03215516-683a-4681-bf41-cbf20acf2225
Stovall K
Review
R Bookmark
RCPS Biology CUA Unit 1/1 of 24
A student carries out an experiment to investigate the role passive transport plays in maintaining homeostasis in organisms For this investigation, the student
uses two gummy bear candies-lo represent animal cells The student records the mass of each bear Then he places one n a beaker filled with distilled water
He places the other in a beaker filled with salt water After 48 hours, he removes the bears from the beakers and records their masses again He finds that the
mass of the gummy bear in the distilled water significantly increased, while the mass of the gummy bear in the salt water significantly decreased
Which of the following is the BEST interpretation of the observations made during the investigation?
O A Distilled water was able to stick to the surface of the gummy bear when it was…
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Answer the question please.
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06:33 t 42 A
Lysosome
63
Compilation of Biochemistry Laboratory Activities Prepared by
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Things to ponder:
1. What particular specimen was used in observing the plant cell using the virtual scope?
Specimen for animal cell?
2. The microscope used in this lab is not powerful enough to view every organelle.
Which organelle(s) can you see in the cheek cell?
3. Name two organelles that are not visible, but should be present in the cheek cell.
4. What cell structures are common to all types of cells?
5. What cell structures are present only in eukaryotic cell?
6. List the structures that are common in both plant and animal cells.
7. List the structures that make plant and animal cells different from each other.
Tabulate your answer.
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What is the answer?
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Title: Osmosis on Zucchini and Cucumber
Objective: Explain how osmosis happens in plants such as zucchini and cucumber.
PLEASE WATCH THE VIDEO LINK HERE BEFORE ANSWERING THE QUESTIONS. Thank you
Youtube link: https://youtu.be/9bPto2f_fGA
After watching it, please ANSWER THE QUESTIONS!
The Questions:
-Explain why and how did this happen to each of the slices of cucumber or zucchini. (In each Set)
-Kindly explain exactly WHY did this happen. (In each Set)
-What type of solution were present in each set up here? (In each Set)
-How did osmosis happen on each of the set ups? (In each Set)
You might be wondering what does it mean with the "(In each Set)". So the format on how you will answer the questions above is in Three sets: Set 1, Set 2 and Set 3 (you can see it in the video, MANDATORY TO WATCH IT).
FORMAT:
SET 1:
Answer the questions.
SET 2:
Answer the questions
SET 3:
Answer the questions
Thank you so much:)
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LAB EXERCISE 3A
A. Cell Membranes. In the square provided, draw a double layer of phospholipids with a large protein
embedded within it. Label the parts of this phospholipid bilayer that are partially charged (polar) and
uncharged (non-polar).
B. Anatomy of a Cell. Draw a "typical" cell in the space below (see lecture notes or the
labeled/unlabeled Images on Canvas). Sketch the following organelles within the cell and label them.
Plasma (Cell) Membrane
Nucleus
Ribosome
Rough Endoplasmic Reticulum
Smooth Endoplasmic Reticulum
Golgi Apparatus
Mitochondria
Lysosome
Cytoplasm
Microvilli
Cilia
A region of exocytosis
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please use hand write
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Question:
Explain how the strongest of the cytoskeletal filaments systems is organized?
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CELL STRUCTURE AND FUNCTION – EUKARYOTES, PROKARYOTES AND VIRUSES-LABORATORY REPORT SHEET
ACTIVITY 2: CELL MEMBRANES, OSMOSIS AND DIFFUSION
Initial Volume (Vi)
(mL)
Final Volume (Vf)
(mL)
Change in Volume
(Vi – Vf)
(mL)
A
20% sucrose
20% sucrose
92.0 mL
94.0 mL
-2.0 mL
B
40% sucrose
20% sucrose
92.0 mL
92.0 mL
0.0 mL
C
20% sucrose
40% sucrose
92.0 mL
90.0 mL
2.0 mL
Treatment
Percent Change
A
-2%
B
0%
C
2%
Hypothesize which dialysis bags should gain, lose or stay the same in regards to volume. Explain.
Which solution is hypotonic, hypertonic and isotonic?
Explain what the change in volume of the dialysis tube indicated and describe what happened when the volume increased and when the volume decreased.
Explain the relationship between solute concentration and amount of free water or water available for movement.
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Abdulla Salem: Attempt 1
E) 20 kilocalories per gram of wax burned
Question 36 (2 points)
lons can travel directly from the cytoplasm of one animal cell to the cytoplasm of an
adjacent cell through
O A) gap junctions.
B) intermediate filaments.
C) plasmodesmata.
D) desmosomes.
E) tight junctions.
Question 37 (2 points)
A.
C.
В.
H.
H
H-C-N
H.
H-C-SH
H-C
H.
H.
H H
hp
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Do explain little.
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I need help labeling this
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Please help :-)
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Label and define the following terms
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Click and drag each label to identify the organelles.
Smooth endoplasmic
reticulum
Proteasome
Golgi complex
Rough endoplasmic
reticulum
Mitochondrion
Centrioles
Inclusion
Lysosomes
Secretory vesicle
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Course X
Edio | Course x
Edio | Course x
A mitochond x Edio Course X
/1089900/lessons/1533965/variants/2428207/take/14/
A
* Practice
TEXT ANSWER
Many different microscopic organisms can be found in pond ecosystems, including the
three organisms shown in the diagrams below. The primary cellular structures in each of
these single celled organisms are labeled in the diagram. Some of the structures are com-
mon to all three organisms and other structures are not. One of the three organisms be-
low can obtain energy through photosynthesis.
a. Based on the diagrams, identify which organism is able to perform photosynthesis.
Explain your reasoning.
b. Identify the two reactants for photosynthesis.
c. At times, this photosynthetic organism can switch to being heterotrophic. Describe a
condition that would favor this organism being heterotrophic. Explain your answer.
Answer using 2 to 3 complete sentences for each question.
Organism 2
Organism 1
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e Final Part 2 (page 2 of 25)
rkanacollege.edu/mod/quiz/attempt.php?attempt=502922&cmid=3353028&page=1
Question 2
Not yet answered
Points out of 1.00
P Flag question
Which structure is the site of the packaging of proteins and lipids that may be shipped out of the
cell? It is the "“Fed-Ex" or "UPS" organelle.
Select one:
O a. Golgi apparatus
O b. tight junctions
O c. rough ER
O d. plasmodesmata
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DELL
->
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#3
$
7
8.
2
3
4
r
y
W
e
k
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MAIN IDEA: Eukaryotic cells have an extensive and dynamic framework called a cyto-
skeleton. The cytoskeleton is used for cell shape, internal structure, movement, and cell
division. Many cells are surrounded by and supported by a complex extracellular matrix and
are able to interact with adjacent cells and the environment via cell junctions.
5. List three common components of microtubules and microfilaments.
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Questions
1. If the potato cells were hypotonic to the surrounding solution, the surrounding solution itself
would be what to the potato cells (hypertonic, hypotonic, or isotonic)?
2. Were the potato cells submerged in water with no salt hypertonic, hypotonic, or isotonic to the
surrounding environment?
3. Were the potato cells submerged in 5 g/100mL salt solution hypertonic, hypotonic, or isotonic to
the surrounding environment?
4.
Look at your graph. At approximately what concentration of salt solution would the potato cells
have likely been isotonic with the surrounding solution? [Tip: Estimate approximately at what
concentration there would have been no change in the average length of potato strips.]
B. Turgor and Plasmolysis
You have observed that concentration differences between cells or between a cell and its environment
can affect the movement of water across cell membranes. This can be observed using changes in volume
or size as in the previous experiment or visually, looking…
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28
Correctly complete the following sentence
Signal Recognition Particles (SRP) enable ribosomes that are making proteins to dock with the endoplasmic
reticulum. A prokaryotic cell that doesn't make this SRP is likely to
Select one alternative:
be unable to produce proteins destined for the endomembrane system or secretion
be unable to secrete proteins
function as normal
start using ribosomes free in the cytosol to produce proteins destined for the endomembrane system.
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You must fill in an answer for (4)-(10)
No need to explain the answers
JUST FILL IN THE BLANKS
THANK YOU. I NEED THE ANSWERS. PLEASE HELP ME OUT
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A.
CELLULAR STRUCTURE AND BIOMOLECULE
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Identify the organelle or cellular structure based on the given reactions. (3 - 10)
3. DNA-directed DNA synthesis
4 DNA-directed RNA synthesis
5. post-translational modification of proteins
6. Glycosylation reaction
7. Lipid synthesis
8. Degradation reactions
9. Catalase activity
10. Glycolysis
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