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Chem 1LE W23 - Thong Minh Tran
Project #4: Gatorade Buffer Capacity/Original Investigation
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Thong Minh Tran
on
Feb 28, 2023 @09:47 PM PST
Table of Contents
Original Investigation
.............................................................................................................................................................................................................................................
2
Original Investigation
Thong Minh Tran - Feb 28, 2023, 9:46 PM PST
Assignment #OI4 - Original Investigation Project #4
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Kim Edwards - Mar 14, 2022, 3:36 PM PDT
Objective
Kim Edwards - Mar 14, 2022, 3:27 PM PDT
The purpose of this lab is to determine the optimal concentration of titrant to titrate Gatorade, the buffer capacity of
Gatorade, and the conjugate base/acid ratio of Gatorade. This can be accomplished by performing titration on Gatorade
with different concentrations of the proper titrant, determined from the initial pH of the Gatorade. The titration data will
be plotted and the buffering region will be used to calculate the buffer capacity and the Henderson-Hasselbach
equation is used to calculate the acid/base ratio of the Gatorade sample. I hypothesize that the titrant with lower
concentration is appropriate for sufficient data set because the reaction will be slower and the buffering region is more
clear.
Thong Minh Tran - Feb 28, 2023, 9:46 PM PST
Project #4: Gatorade Buffer Capacity/Original Investigation
2 of 9
Safety Moment
Kim Edwards - Mar 14, 2022, 3:27 PM PDT
Exposure Routes
Inhalation
1. You are done pumping gas and pulling the nozzle out of the car’s tank but the gas continues to flow spraying
you in the face. Your eyes start to burn and you have to remove your contacts.
2. You choose a beaker without realizing it has a small star crack in the bottom. The beaker is filled halfway with
methanol and placed on the hotplate which is turned on to heat the solution. The beaker shatters as you pick it
up, cutting your hand.
3. You are distracted when you enter the lab and forget to cover your laptop keyboard with plastic wrap. Later
that day you are at the library studying and you realize a solid is on your trackpad. You lick your finger to wet the
trackpad in an attempt to remove the solid. It doesn’t come off the first time, so you lick the finger again. Your
mouth becomes numb.
4. It’s your turn to clean the bathroom at the apartment. You start out using ammonia, but the smell is terrible, so
you switch to bleach without washing away all the ammonia. Pretty soon you eyes are watering and you are
coughing badly.
What chemical or physical properties
or
procedure steps in the lab might increase the possibility of exposure by
each route? (Hint: If you have absorption, skin is arranged in a system of stacked lipid bilayers. What does that
indicate about the polarity of your skin surface?)
Aqueous or gaseous chemicals that emit vapor would increase the possibility of exposure for chemical inhalation.
How do you minimize exposure via this route?
To minimize chemical inhalation, ensure that all chemical containers are properly sealed and use the fume hood
when dealing with liquid or vapor chemical.
What do you do if you are exposed to a hazardous chemical via this route?
Get fresh air and seek medical professionals.
Which of the following incidents was your exposure route most likely? Describe the exposure & aftermath in terms
of the first 3 questions.
Situation number 4 corresponds the most to inhalation because the bleach vapor is being inhaled, which caused
watering eyes and cough.
Thong Minh Tran - Feb 28, 2023, 6:31 PM PST
Project #4: Gatorade Buffer Capacity/Original Investigation
3 of 9
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Procedures & Observations
Kim Edwards - Mar 14, 2022, 3:27 PM PDT
Procedures:
Observations:
Checking pH Probe Accuracy
1. Obtain 2 pH probes for two pairs of students in each
group. Plug the pH probe into LabQuest 3 using channel 1
port or USB port.
Set 1: LabQuest# 58, pH Probe# 32
Set 1: LabQuest# 8, pH Probe# 21
2. Take pH probe out of storage solution and rinse with DI
water. Blot dry with Kim wipes.
pH 7 buffer is liquid, yellow, transparent, and odorless.
3. Obtain about 10mL of pH 7 buffer in a 50 mL beaker.
pH reading has 2 significant digits.
4. Dip the entire bulb of pH probe into the buffer and wait the
reading to stabilize. The pH should be 7 +/- 0.4. Use new pH
probe if reading is off.
Finding Buffer Capacity
5. Obtain 10mL of Gatorade and record the pH
Gatorade pH= 3.05
Gatorade is liquid, red, transparent, and odorless.
6. Decide the titrant based on the pH of the Gatorade
Since the pH of the Gatorade is 3.05 which is acidic, the
titrant would be NaOh which is basic.
7. Obtain two 250 mL beakers, each holding 25mL of
Gatorade
8. For titrants, perform five-fold dilution once to obtain 2
solutions of titrants with different concentration. Transfer 20
mL of stock solution in to a 100mL Graduated cylinder with a
10mL volumetric pipet. Add DI water until the total volume
reach 100mL. Measure the pH for the NaOH stock solution
and the diluted solution
NaOH solution is liquid, colorless, transparent, and
odorless.
Stock solution: 0.5M NaOH
Diluted solution: 0.1M NaOH
M
*V
= M
* V
V
= (M
* V
)/M
V
=(0.1M*100mL)/0.5M
V
= 20mL
pH of 0.5M NaOH= 13.63
pH of 0.1M NaOH= 12.46
9. There will be 2 different solutions, one for each titration
Titration 1: Gatorade with 0.5 M NaOH
Titration 2: Gatorade with 0.1 M NaOH
10. To prepare buret for titration, add about 10mL of DI water
into buret, turn the stopcock to close position and observe for
leaks.
11. Pre-rinse buret with the titrant to be delivered twice.
Close stopcock and use a funnel to add <5mL of titrant while
holding the buret in front of face. Tilt and rotate buret to wet
the interior surface. Place buret in buret stand and drain
titrant into a waste beaker. Close buret.
12. Fill the buret with titrant above the 29mL mark and drain
into a beaker to remove air bubles and the initial volume
reaches 30.00mL. Record initial volume:
Thong Minh Tran - Feb 28, 2023, 9:32 PM PST
conc
conc
dil
dil
conc
dil
dil
conc
conc
conc
Project #4: Gatorade Buffer Capacity/Original Investigation
4 of 9
13. To perform the titration, place the beaker with the buffer
solution onto a magnetic stir plate and add a small Teflon
bar. Adjust the stirrer to a slow stirring speed.
14. Clamp the pH probe to the side of the beaker to not
collide with the stir bar and impact to the beaker.
15. On Labquest3, select Sensors>Data collection>Events
with Entry. Type "Acid Added" or "Base Added" in Name field
and change unit to mL and press OK. Press Go when ready
to collect data. One pH is stabilized, press Keep. Press 0 for
entry and press OK again.
16. For NaOH titrant, repeat the process until a repeatedly
high pH is reached (pH>12). For HCl titrant, repeat the
process until a repeatedly low pH is reached (pH<3).
17. When finished, press Stop and ask Ta to check the
graph.
18. Add title and axis label to the graph.
19. To clean up, drain all titrant into waste beaker. Rinse pH
probe with DI water and place into storage solution. Retrieve
the stir bar from the solution using the magnetic stir bar
retriever. Rinse beaker, buret, and stir bar with DI water from
squeeze bottle.
20. Calculate the buffer capacity for Gatorade
Sample Calculations:
The buffering region is between 1mL and 10mL of titrant
added.
β=Δmoles
/ΔpH
β=Δvolume
*Concentration
/ΔpH
β=(10mL-1mL)*(1L/1000mL)*0.1M / (6.45-3.34)
β= 2.90*10
21. Obtain the pKa by locating pH of the solution at the
volume halfway from the equivalence point.
Since the equivalence point is when 12mL of titrant
added, the pKa of the Gatorade is the pH of the analyte
solution at 6mL of titran added.
pKa= 5.02
22. Use the pKa from step 20, the pH from step 5, and the
Henderson-Hasselbach equation to calculate the conjugate
acid/base ratio
pH = pKa + log ([A ]/ [HA])
[A ]/ [HA]=10
[A ]/ [HA]=10
[A ]/ [HA]= 0.0107
[HA]/[A ] = 1/0.0107= 93.33
23. Transfer remaining Gatorade into the waste beaker. Add
Citric acid or Sodium bicarbonate into solution and check pH
with pH paper. Pour the solution down the drain once the pH
is around 7.
titrant
titrant
titrant
4
-
-
pH-pKa
-
3.05-5.02
-
-
Project #4: Gatorade Buffer Capacity/Original Investigation
5 of 9
Dilution Plan:
Solution
Concentration
of
solution being
diluted
(M_conc)
(M;molar)
Final solution
Concentration
(M_dil)(µM;
molar)
Graduated
Cylinder
Volume
(V_dil)(mL)
A
0.5
0.1
100
Titration Curves:
For 0.50M NaOH titrant:
For 0.10M NaOH:
Thong Minh Tran - Feb 28, 2023, 9:27 PM PST
Project #4: Gatorade Buffer Capacity/Original Investigation
6 of 9
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For Group Data Tables and Graphs:
https://docs.google.com/spreadsheets/d/1P5e7M1QYLS7RyVir-daArkhvKbbrGfIao19LayDPACQ/edit#gid=1925661601
For Class Data:
https://docs.google.com/spreadsheets/d/138c3DojtX1okAbZrJZyjlHlQffrBKTxndxr41znoSQk/edit#gid=1747521696
Thong Minh Tran - Feb 28, 2023, 8:26 PM PST
Project #4: Gatorade Buffer Capacity/Original Investigation
7 of 9
Poster
Kim Edwards - Mar 14, 2022, 3:27 PM PDT
Thong Minh Tran - Feb 28, 2023, 9:37 PM PST
Project #4: Gatorade Buffer Capacity/Original Investigation
8 of 9
Critique & Claim Evaluation
Kim Edwards - Mar 14, 2022, 3:27 PM PDT
Original Investigation
: Gatorade will be available in the lab. What concentration of titrant is appropriate for a
sufficient data set? What is the buffer capacity and HA/A
-
or B
-
/HB ratio of your Gatorade sample?
Groups
Gatorade
Gatorade pH
Titrant(s)
β
[HA]/[A-]
Concentration pKa
1 red
3.05 NaOH
2.90E-04
93.3
0.1
5.02
2 red
3.41 NaOH
4.23E-04
38.9
0.1
5
3 red
3.54 NaOH
2.60E-03 n/a
0.1
5
4 red
3.5 NaOH
5.58E-04
31.6
0.1
5
5 red
3.7 NaOH
0.23
33.89
0.125
5
6 red
3.42 NaOH
3.00E-04
38
0.25
5
The concentration of titrant that is appropriate to titrate Gatorade is 0.10M of NaOH. The buffer capacity of Gatorade is
0.00029 and the [HA]/[A ] ratio is 93.33. This is the original claim and was not changed after the Argumentation section.
All the other groups also used the red Gatorade and most groups used the 0.1M NaOH as titrant. Our acid/base ratio is
93.33 which is higher compared to most of the other groups with their values around 30, this is because our initial pH of
Gatorade is lower compared to the other groups which resulted in a higher acid/base ratio. Our value of buffer capacity
is still unaffected by the initial pH and is comparable to other groups.
Thong Minh Tran - Feb 28, 2023, 9:43 PM PST
-
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- 1. Solid Lead react with Copper (II) nitrate Please write Complete reaction / Ionic reaction Net ionic reaction Oxidized half Reduced half Find oxidation agent/ Find reduction agent/ ................................................................................ Balan2. Balance the following redox reaction using the half-reaction method under basic conditions. MnO4- + 2CHCl3 ® 3Cl2 + 2CO2 + Mn2+arrow_forwardHydrochloric acid (aq) reacts with the mineral calcite (s) to produce carbon dioxide (g), water (l), and calcium chloride (aq). Based on what you have learned in activities 1, 2, and 3, what are three things you could do to make the reaction occur more quickly? .,.,.,.,.,.,.,.,.,.,.,.,.,.,.,.,.,.,.,.,..,.,,.,arrow_forwardLUTCA Chapter 3 Homework: Allie Burt ← Chapter 3 Homework Question 13 of 14 View Policies Current Attempt in Progress r Save for Later L WP NWP Assessment Player Ul Application education.wiley.com g of malachite were originally used. Chemistry I (Science Majors) (CHEM-141... Ć Malachite is a green copper mineral with the formula Cu₂CO3(OH)2. It can be converted into copper metal in a number of ways. One method extracts the metal by reacting the malachite with sulfuric acid to form CuSO4, and then adding iron to the CuSO4 solution which, after they react, forms FeSO4 and copper metal. The balanced chemical reactions are: Cu₂CO3(OH)2 (s) + 2 H₂SO4 (aq) →2 CuSO4 (aq) + 3 H₂O (1) + CO₂ (g) CuSO4 (aq) + Fe (s) →FeSO4 (aq) + Cu (s) If the final solution is dried, and 3.28 g of iron sulfate is collected, how many grams of malachite were originally used? W Wiley Course Resources -/1 = : Attempts: 0 of 3 used Submit Answer Quest Multipar Quest Multiple Ques Numer Ques Numer Units Que Multip Que…arrow_forward
- x CHE101_02: Intro to General Che X Home 101 Chem101 My Questions | bartleby app.101edu.co myClackamas Login CUnofficial Transcri... W Logon Oregon Scholarsh... Home FAFSA on... Welcome to the ... The National Soci... Apps Submit Question 5 of 8 How many moles of lithium hydroxide would be required to produce 75.5 g of Li2COs in the following chemical reaction? 2 LIOH(s)+CO2 (g) --- LI2CO3 (s) + H2O (I) mol 1 2 с 7 +- 0 x 10 0 7:27 PM Type here to search ENG 11/13/2019 X CO LO 4tarrow_forwardI need help! Plsarrow_forwardnot use ai pleasearrow_forward
- I understand how to solve the questions, but in the overall question how do we know that the unknown metal compound would not be included in the Ksp expression? We are assuming that the unknown metal compounds are solid but why? And if they are solid because of solubility rules, wouldn't that mean that they would not ionize in solution? I hope this question makes sensearrow_forwardFor each reaction, identify the reactant as an acid , a base , or neither an acid or base : a. HCl + H 2 O → H 3 O + + C l - , HCl is a/an ................................ ............... acid base neither b. NaCl + H 2 O → Na + (aq) + Cl - (aq), NaCl is a/an ................................ . acid base neither c. NaOH (s) → Na + (aq) + OH - (aq), NaOH is a/an ................................ . acid base neithearrow_forwardWhen plotting the standard calibration curve for equilibrium constants the independent variable is ........ and is plotted on the .......... while the dependent variable is and is plotted on the ......... options = (volume, mass, percent by mass, density, concentration, absorbance )arrow_forward
- Aktiv Chemistry Success Confirmation of Question X ← → C app.101edu.co https://canvas.tamu... Jobs For Aggies - H... 76°F Mostly cloudy Jobs - Zoo Jobs Ne... NaOH Draw the product of the E2 reaction shown below. Ignore any inorganic byproducts. + CI Internships | Job Ca... internship paper Your Online Accoun... Question 9 of 21 99+ Urban Wildlife Infor... A Site Suitability An... Please select a drawing or reagent from the question area Aktiv Learning 4 D Submit 4:10 PM 10/31/2022 X : sarrow_forwardCan you please complete and check if the answers are correct!!! Thank you in advance!!arrow_forwardA learning.mheducation.com Types of Reactions assignment and quiz F. Question Mode: Multiple Select Question Explain the different types of chemical react... https://moodle.coastal.edu/pluginfile.php/106... https://moodle.coastal.edu/pluginfile.php/106... Exit Assignment X Mc Graw Hill 13 of 36 Concepts completed i Multiple Select Question Select all that apply LI Which of the following reactions are expected to occur as written, with Ba reference to the activity series for metals (shown)? Select all that apply. Ca O Cu (s) + 2HCI (aq) → CuCl2 (aq) + H2 (g) Na Mg Mg (s) + Co(NO3)2 (aq) → Co (s) + Mg(NO3)2 (aq) Al Mn O Pb (s) + 2HCI (aq) → PbCl2 (s) + H2 (g) Zn Cr O 3Ag (s) + Fe(NO3)3 (aq) → 3ÄGNO3 (aq) + Fe (s) Fe NI Sn Pb H2 Cu Hg Ag Au Copyright O McGraw-Hll Education EText Alternative Need help? Review these concept resources. E Reading Rate your confidence to submit your answer. High Medium Low O2021 McGraw-Hill Education. All Rights Reserved. Privacy Terms of Use 80 888 DII DD F10…arrow_forward
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