Food Dyes Analysis of Commercial Products Part 2 (1)

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University of South Florida *

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2045L

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Chemistry

Date

Apr 3, 2024

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pdf

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4

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Food Dyes Analysis of Commercial Products Part 2 CHM2045L.017S24 Mar 28, 2024 Taylor Tran Annabelle Martin Sophia Bates Maadh Alshaaili Objective: The purpose of this experiment is to understand and apply the principles of spectrophotometry for chemical analysis. We will also need to be able to explain the interaction of UV-visible radiation and molecules in commercial products. Creating and understanding a calibration curve is also required. Alongside the calibration curve, understanding the electromagnetic spectrum, light wavelength and color, UV-Visible absorption spectroscopy, spectrophotometer, concentration of solutions, and dilution will also be needed. Materials: 25mL volumetric flask 100mL beaker 7mL Graduated Pipettes 10mL Blue Powerade 10mL Loco Mango Monster Energy 10 mL Blue Gatorade 10 mL Fruit Punch Gatorade Pipette pumps Cuvettes Pasco Scientific Vis-Spectrophotometer Chemicals/Hazards: Chemical Name Chemical Formula Molecular Formula Weight Physical Appearance Specific Hazards Dihydrogen monoxide H 2 O 18.01528 g/mol Clear liquid N/A Red #40 C 18 H 14 N 2 Na 2 O 8 S 2 498.44 g/mol Dark Red Powder May cause eye irritation. May cause gastrointestinal
discomfort. May cause irritation to the respiratory tract. May cause irritation to skin. Blue #1 C 37 H 34 N 2 O 9 S 3 Na 2 792.86 g/mol Dark violet Powder May cause eye irritation. May cause gastrointestinal discomfort. May cause irritation to the respiratory tract. May cause irritation to skin. Methods/Procedures: 1. Choose TWO food dyes that you want to work on from the list below: - Blue #1 - Red #40 2. Choose a LIQUID commercial food product that contains the food dye you chose. Write the name of the products here and the food dyes they contain. (Make sure to read the ingredient list for the color and number associated with the food dye). Blue Powerade: Blue #1 Blue Gatorade: Blue #1 Loco Mango Monster Energy: Red #40 Fruit Punch Gatorade: Red #40 3. What are the lambda max for the TWO food dyes you chose? Blue #1: 598.4 nm Red #40: 436 nm 4. What is an acceptable range for absorbance values? Blue #1: 0.370 to 0.991 Red #40: 0.027 to 0.338 5. What will be used as the blank? DI water filled cuvette
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