001Lab Report Thin Layer Chromatography

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Chemistry

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Apr 3, 2024

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Lab Report: Thin Layer Chromatography Handout: Understanding the principles of Organic Chemistry – A Laboratory Experience; Brooks/Cole Cengage Learning; Steven F. Pedersen and Arlyn M Myers; 241-245 Reading assignment: Mohrig, J.R.; Hammond, C.N.; Schatz, P.F.; Techniques in Organic Chemistry; Freeman, New York, 2011, 221-235 (3 rd Edition) **When writing on your lab report, be aware that your work will be checked against all the students of all the course sections, including past sections. You can have your friends to help you brain storm the assignment, but plagiarized work and identical/partially identical work will receive an F grade.** _____/30 Experiment Title: Thin Layer Chromatography in Organic Chemistry (0/30 points) TLC plates: Attach a picture of your your TLC plates for this experiment. Be sure to report the solvent system for each plate and the distances marked for the calculation of Rf. TAs must know what each spot is! Make sure to have a legend (4/30 points) Enter your illustration or TLC plates pictures here
Results : Report clearly in tables the distances traveled by the solvent and each of your control and your unknown sample. Show clearly the Rf calculations for each solvent system. (10/30 points) Beaker 1: 60:40 hexane: ethyl acetate Solvent traveled Distance: Spots Distance Traveled Rf A 1.6 0.35 C 0.9 0.20 T 2.9 0.64 Unknown 3.0 0.66 Rf Calculations: 1.6/4.5=0.35 0.9/4.5=0.20 2.9/4.5=0.64 3.0/4.5=0.66
Beaker 2: 10:10:1 toluene: ethyl acetate: acetic acid Solvent traveled Distance : Spots Distance Traveled Rf A 2.1 0.35 C 1.4 0.23 T 4.7 0.78 Unknown 5.7 0.95 Rf Calculations: 2.1/6.0=0.35 1.4/6.0=0.23 4.7/6.0-0.78 5.7/6.0=0.95 Beaker 3: 92: 80 ethyl acetate: acetic acid Solvent traveled Distance: Spots Distance Traveled Rf A 0.4 0.06 C 1.5 0.25 T 1.9 0.32 Unknown 3.0 0.50 Rf Calculations: 0.4/6.0=0.06 1.5/6.0=0.25
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