Final_Exam

.pdf

School

California State University, Long Beach *

*We aren’t endorsed by this school

Course

441A

Subject

Chemistry

Date

Jan 9, 2024

Type

pdf

Pages

Uploaded by BailiffLlamaMaster257

1 CHEMISTRY 441A FINAL EXAM SET A Dec 14, 2017 S Manandhar N ame: ________________________________Student ID#: ____________________________ Instructions: 1. DO N OT begin the exam until instructed to do so. 2. SWITCH OFF your phones. 3. Make sure there is a total of 12 pages . 4. Write your N ame AND Exam number (A or B) on your Scantron. 5. When finished, place Exam sheet and Scantron and return all materials to the instructor. Summary of points: 1. Answer on Scantron Section A (Q# 1….60) (60 Points) 2. Answer on the Exam Sheet Section B, C, and D (Q# 61-90) (40 Points) 3. Answer on the Exam Sheet Section E (Q# 91-93) (6 Points) 4. TOTAL 100 + 6 points A B ______ C ______ 4 T O T A L Jerry Litteral 694205318008 1000 Shanna

2 SECTION A – Please, answer these questions on Scantron (1x60 = 60 points). 1. In the figure below, where is a hydrogen bond acceptor located? a) B b) C c) D d) A 2. )RU $73 ±VKRZQ EHORZ²³ FOHDYDJH RI WKH ERQG EHWZHHQ BBBBBBBBBB KDV WKH ODUJHVW ǻ* ƍ . a) adenine and ribose b) ȕ SKRVSKDWH DQG Ȗ SKRVSKDWH c) Į SKRVSKDWH DQG ȕ SKRVSKDWH d) ULERVH DQG Į SKRVSKDWH 3. All of the following are important for enzyme-substrate interactions EXCEPT a) hydrogen bonds. b) van der Waals interactions c) ionic interactions. d) covalent bonds. 4. When a protein folds in an aqueous solution, which of the following is correct? a) A mixture of polar and non-polar amino acids is found in the interior of the protein. b) Polar amino acids are found in the interior of the protein c) Water is always excluded from the active site in the interior of the protein. d) Non-polar amino acids are found in the interior of the protein. 5. Which of the following is correct about biological membranes? a) Phospholipid tails are directed toward the aqueous environment b) Biological membranes are made up of a lipid monolayer. c) Phospholipid head groups are directed toward the aqueous environment. d) Molecules freely pass through the membrane. 6. Which situation described below would result in the MOST rigid biological membrane? a) A high ratio of cholesterol b) A large number of bulky, glycosylated head groups c) A high ratio of unsaturated fatty acid tails in the phospholipids d) A small amount of cholesterol 7. Which of the following three-letter amino acid sequence match with this single-letter sequence FYAKGPE? a) Phe-Tyr-Asp-Lys-Gln-Phe-Glu b) Phe-Tyr-Asn-Lys-Gly-Pro-Glu c) Phe-Tyr-Ala-Lys-Gly-Pro-Glu d) Phe-Tyr-Ala-Lys-Glu-Phe-Gln IEent kind of bond Phosphoanhydrid o bonds I fi skIstr eEthe0x roar exy E x.EE S p Ix only some saw not enough for significance F Phenyl TX XP pre A 11 Jok Lys G Fin rated E 6 13

3 8. Transformation is a transfer of DNA a) Mediated through viral infection b) Mediated through bacterial mating c) Mediated through uptake of exogenous DNA from the medium d) All the above 9. Which of the following nucleotides are used in Sanger method of DNA sequencing? a) Deoxyribonucleotides b) Ribonucleotides c) Dideoxyribonucleotides d) Diribonucleotides 10. Which of the following is the correct order for DNA condensation in eukaryotes? a) Nucleosomes, chromatin, loops, condensed coils, chromosome b) Chromatin, nucleosomes, loops, condensed coils, chromosome c) Nucleosomes, condensed coils, loops, chromatin, chromosome d) Chromatin, nucleosomes, condensed coils, loops, chromosome 11. The figure below shows a nucleosome core particle. Which of the following is INCORRECT? a) H1 histone protein is not part of the histone protein core. b) The histone protein core consists of eight histone proteins. c) DNA wraps around the histone protein core twice. d) The interactions between the DNA and the histone protein core are sequence specific. 12. Which of the following is considered gene-rich chromatin? a) Euchromatin c) Heterochromatin b) Centromeres d) Telomeres 13. Which of the following is true for polycistronic genes? a) The coding sequences are randomly organized b) Each coding sequence has its own promoter c) The sequence can be an operon, coding for several proteins of a single biochemical process or pathway. d) The promoter is located downstream of the coding sequence. DNA for DNA only right statement RELAX ARNAX even a thing must start 0 vi loops make oil w nucleosomes II es ota 0 rissed condensed non coding bothtypes of heterochromatin one promoted multiple regions Poly multiple Istream One promoter multiple coding regions operon proteins in a single pathway

4 14. Iodine and Na + are transported in the same direction and have an overall – ǻ G . This occurs in a) passive symporter b) primary active transporter c) secondary active symporter d) secondary active antiporter 15. Sigmoidal curve of hemoglobin saturation is due to a) Multiple subunit compositions b) Cooperativity c) Low affinity to O 2 d) All of the above 16. Which of the following is INCORRECT regarding eukaryotic genes? a) 7KH ±ƍ 875 DQG ²ƍ 875 DUH QRW WUDQVODWHG EXW SOD\ LPSRUWDQW UHJXODWRU\ UROHV b) 0DWXUH 51$V KDYH D ±ƍ FDS DQG D ²ƍ poly(A) tail c) Exon sequences are removed from the primary transcript prior to translation. d) Alternative splicing allows for one gene to produce several different protein products 17. Which component of a common plasmid cloning vector is necessary for blue-white screening? a) lacZ gen c) Antibiotic-resistance gen b) Multiple cloning sites (MCS) d) Origin of replication 18. Which of the following is correct about restriction enzymes? a) All restriction enzymes produce the same type of cleaved ends b) Type II and Type III restriction enzymes cut outside of their recognition sequences. c) Type II restriction enzymes recognize palindrome sequences. d) Restriction enzymes degrade foreign DNA by cleaving methylated DNA 19. The figure below shows the temperature cycling used for a typical PCR reaction. Which of the following is occurring at step B? a) Denaturation of target DNA b) Annealing of primers to target DNA c) Extension of primers d) Denaturation of primers 20. Which of the following is NOT true for Sanger DNA sequencing compared to Maxam-Gilbert DNA sequencing? a) Sanger DNA sequencing is a high efficiency method of DNA sequencing. b) Sanger DNA sequencing relies on chemical degradation of DNA. c) Sanger sequencing is also called chain termination sequencing. d) Sanger DNA sequencing uses ddNTP Eno ATP anti same dirxn heme units E O 0 Intestine sina.EE stick Titan Imethated Item Temp Iem relies on this

5 21. Which of the following is correct concerning a peptide backbone? a) The peptide bond allows for free rotation due to its single bond character b) A Ramachandran plot shows the allowable ࢥ DQG ȥ DQJOHV IRU D SHSWLGH c) The torsional angle between the C Į and the R group is called ࢥ (phi). d) The torsional angle between the amide nitrogen and the C Į LV FDOOHG ȥ ³SVL´ 22. A __________ mutation will not lead to a change in the protein product of a gene. a) Frameshift b) silent c) nonsense d) missense 23. Disulfide bonds stabilize __________ level of protein structure and above. a) Quaternary b) tertiary c) primary d) secondary 24. In the figure shown below, which of the following is labeled “B”? a) $QWLSDUDOOHO ȕ VWUDQG b) ȕ WXU n c) Loop d) 3DUDOOHO ȕ VWUDQGV 25. In the figure shown below, C is which of the following classes? a) 3UHGRPLQDQWO\ Į KHOL ces b) Įµ ȕ c) Į ¶ ȕ d) 3UHGRPLQDQWO\ ȕ VKHHWV 26. An antigen binds to an antibody at which of the following locations. a) Only at the light chain b) Only at the heavy chain c) At the constant domain d) At the variable domain 27. For Anfinsen’s studies of RNaseA folding, which of the following is not correct? a) ȕ -mercaptoethanol was used to reduce disulfide bonds. b) 5HPRYDO RI ȕ -mercaptoethanol, followed by removal of urea, resulted in active RNaseA. c) Removal of ȕ -mercaptoethanol and urea at the same time resulted in active RNaseA. d) Urea was used to disrupt polar interactions within the protein. 28. Which of the following protein-folding diseases is a loss-of-function disease? a) Huntington’s disease b) Alzheimer’s disease b) Cystic fibrosis c) Creutzfeldt-Jakob disease 2x bond EFI 1 de n or insertion Eagle nut changer Effans Hands loop A helices β sheet NB d B domains are in these I S es in Inactive s ty again toxicity damage Godin misfolding loss OR CFTR function

Your preview ends here

Eager to read complete document? Join bartleby learn and gain access to the full version