Experiment1_SimoneDo_14186997_250822

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University of Technology Sydney *

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2

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Electrical Engineering

Date

Feb 20, 2024

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pdf

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7

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FOUNDATION EXPERIMENT 1: EXTERNAL CALIBRATION -HPLC Name: Simone Do Student Number: 14186997 Date of Submission:25/08/2022 Date of Practical Session:11/08/2022 Experimental unknown Code: U2006 HPLC column:C18 5 μm 3.9x150mm Column Mobile Phase:65% Water 35% Methanol Injection volume:5.0 mL Mobile phase flow rate:1.000 mL/min Detector: Diode Array Detector Detection Wavelength:273nm and 254nm Column Temperature:30.0°C Data file directory: 20220811_AC2 Results Part 1: Standards Table 1: Concentration standards. Volumetric Flask Standard Concentration (ppm) Volume of 1000-ppm stock to be added (μL) 1 2 20 2 4 40 3 6 60 4 8 80 5 10 100 6 12 120
Part 2 : Standard blank Figure 1: Chromatogram of instrument blank.
Part 3: Identification of peaks Figure 2: Chromatogram of Spike T (theophylline) indicating both peak 1 and 2. Figure 3: Chromatogram of Spike C (caffeine) indicating both peaks 1 and 2.
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