Abstract:. Diffusion Occurs As Passive Transport In Cells

In this lab experiment, half our group observed and measured osmosis using dialysis tubes that were represented as the semipermeable membrane. It is permeable to water and other small molecules but is impermeable to larger molecules such as the sucrose solution used in each of the four beakers and tubing. The other half of our group observed the tonicity of sheep blood to determine whether the blood was isotonic, hypotonic, or hypertonic. The 85 g/dL of NaCl solution was the ideal isotonic number in relation to the sheep blood cells as well as a reference to the other observations of the solutions.

1) The relationship between the rate of diffusion, volume and surface area is that the surface area of a subject somewhat correlates with the volume of the object. When the surface area and volume are set up as a ratio, this gives a good idea as to how fast the rate of diffusion will be. The higher surface area to volume ratio, the faster the rate of diffusion. This is because then there is more surfaces to diffuse through and there materials to diffuse, the rate of diffusion is a lot faster. Conversely, when there is a smaller surface area for more molecules to diffuse through, the rate of diffusion will be lower.

All cells contain membranes that are selectively permeable, allowing certain things to pass into and leave out of the cell. The process in which molecules of a substance move from an area of high concentration to areas of low concentration is called Diffusion. Whereas Osmosis is the process in which water crosses membranes from regions of high water concentration to areas with low water concentration. While molecules in diffusion move down a concentration gradient, molecules during osmosis both move down a concentration gradient as well as across it. Both diffusion, and osmosis are types of passive transport, which do not require help.

Cells and molecules in the environment are constantly moving and changing, for cells to function properly there is a need for equilibrium to be met. The size of the cell and the solution outside of the cell affects the rate of diffusion and osmosis in the cell. Cells are constantly trying to reach an equilibrium with the molecules and substances around it, which is why there are such terms as: hypertonic, hypotonic and isotonic. The procedures allowed testing of whether or not surface area or volume increased diffusion and how different substance control diffusion. Cells are constantly moving to reach equilibrium through diffusion and osmosis.

Dialysis tubing is a membrane made of regenerated cellulose fibers formed into a flat tube. If two solutions containing dissolved substances of different molecular weights are separated by this membrane, some substances may readily pass through the pores of the membrane, but others may be excluded.

Diffusion is the movement of molecules from a region of higher concentration to a region of lower concentration. The rate at which molecules diffuse can be determined by the relationship of molecular weight and that rate of diffusion through a membrane. Hypothesis of this experiment is that the fluid with higher molecular weight will diffuse at a slower rate and distance.

With all solutes set at a concentration of 5.00 mg/ml and the MWCO set at 20, filtration stopped at 60 minutes, and the projected completion was 100 minutes. The residue analysis indicated all solutes present in the dialysis membrane. The filtrate concentrations for all solutes was 0.00 mg/ml. With all solutes set at a concentration of 5.00 mg/m and the MWCO set at 50, the filtration completed in 40 minutes. The residue analysis indicated all solutes present in the dialysis membrane. The filtrate concentration for NaCl was 4.81 mg/ml, and 0.00 mg/ml for all remaining

The difference is that along with large molecules, living cells prevent molecules with positive charges and solubility. This is not representing in dialysis tubing, and is only found in living cells because the tubing is only based on molecular size (98). When referring the rate of diffusion, the concentration gradient influences the diffusion rate, based on the factors of temperature. The ability for molecules diffuse from high to low concentrations primarily depends on the concentration gradient between the two areas.(96-99). My hypothesis for the study is that in the hypotonic, hypertonic, and isotonic solutions, the direction and rate of osmosis will determine based on the concentration inside the dialysis tubing. My prediction is that if the solution is hypotonic the results will decrease, if the solution is hypertonic the results will increase and if the solution is isotonic the solution will vary and or remain constant.

A major determinant of diffusion in a biological system is membrane permeability. Small, uncharged molecules pass through cellular membranes easily, while most and/or charged molecules cannot pass through the membrane. The movement of water across a selectively permeable membrane, like the plasma membrane

We filled bags of dialysis tubing with 20 ml of sucrose, NaCl, ovalbumin, and glucose and weighted them. After soaking them in distilled water for 10 minutes we measured them again. All of the bags gained water. Our hypothesis was that water will diffuse into the cells with ovalbumin, NaCl, glucose, and sucrose, but not the bag with distilled water. The independent variables were the different solutes in the dialysis tubing. The dependent variable was if the water would diffuse into the

The dialysis membrane’s permeability was tested with four substances. Starch, sulfate ion, chloride ion, and albumin are all substances of different sizes. Albumin is a protein that is fairly large so it cannot diffuse through a semi-permeable membrane. Ions such as the sulfate and chloride ion are small enough that they can go through the microscopic pores of the dialysis tubing.

The following hypothesis was made in regard to effect of the concentration gradient on the rate of diffusion: The higher the concentration gradient, the faster the rate of diffusion.

diffusion is one of the passive transport processes. it is used in oxygen entering a cell and carbon dioxide leaving a cell. diffusion is the movement of particles such as atoms or molecules from a high concentration place in an area of a low concentration. this shows that they diffuse down the concentration gradient. the concentration gradient is a gradual change in the concentration of solutes in a solution as a function of distance through a solution. in biology a gradient results from an unequal distribution of particles across the cell membrane. When this happens solutes move along the concentration gradient until the concentration of the

The dialysis tubing experiment will test the effect that different concentrations of sucrose within the semi-permeable membrane will have on the net direction of water diffusion. The solutions within the

Diffusion is the passage of solute molecules from an area of high concentration to an area of low concentration (Campbell & Reece, 2005). An example is ammonia diffusing throughout a room. A solute is one of two components in a chemical solution. The solute is the substance dissolved in the solution. The solvent, the other component, is any liquid in which the solute can be dissolved (Anderson, 2002). Diffusion requires little or no energy because molecules are always randomly moving; this is due to their kinetic energy. Diffusion occurs only when there is an imbalance in the areas of