Abstract:. Diffusion Occurs As Passive Transport In Cells

Cells and molecules in the environment are constantly moving and changing, for cells to function properly there is a need for equilibrium to be met. The size of the cell and the solution outside of the cell affects the rate of diffusion and osmosis in the cell. Cells are constantly trying to reach an equilibrium with the molecules and substances around it, which is why there are such terms as: hypertonic, hypotonic and isotonic. The procedures allowed testing of whether or not surface area or volume increased diffusion and how different substance control diffusion. Cells are constantly moving to reach equilibrium through diffusion and osmosis.

If the solution in the left beaker contained both urea and albumin, which membrane(s) could you choose to selectively remove the urea from the solution in the left beaker? How would you carry out this experiment?

In this lab experiment, half our group observed and measured osmosis using dialysis tubes that were represented as the semipermeable membrane. It is permeable to water and other small molecules but is impermeable to larger molecules such as the sucrose solution used in each of the four beakers and tubing. The other half of our group observed the tonicity of sheep blood to determine whether the blood was isotonic, hypotonic, or hypertonic. The 85 g/dL of NaCl solution was the ideal isotonic number in relation to the sheep blood cells as well as a reference to the other observations of the solutions.

The purpose of these experiments is to examine the driving force behind the movement of substances across a selective or semiperpeable plasma membrane. Experiment simulations examine substances that move passively through a semipermeable membrane, and those that require active transport. Those that move passively through the membrane will do so in these simulations by facilitated diffusion and filtration. The plasma membrane’s structure is composed in such a way that it can discriminate as to which substances can pass into the cell. This enables nutrients to enter the cell, while keeping unwanted substances out. Active

Two variables that affect the rate of diffusion are the MWCO membrane and the solute concentration. Increasing the membrane size and solute concentration will also increase the average diffusion rate. Decreasing the membrane size and solute concentration will reduce diffusion rates and can even prevent all diffusion.

diffusion is one of the passive transport processes. it is used in oxygen entering a cell and carbon dioxide leaving a cell. diffusion is the movement of particles such as atoms or molecules from a high concentration place in an area of a low concentration. this shows that they diffuse down the concentration gradient. the concentration gradient is a gradual change in the concentration of solutes in a solution as a function of distance through a solution. in biology a gradient results from an unequal distribution of particles across the cell membrane. When this happens solutes move along the concentration gradient until the concentration of the

Dialysis tubing is a membrane made of regenerated cellulose fibers formed into a flat tube. If two solutions containing dissolved substances of different molecular weights are separated by this membrane, some substances may readily pass through the pores of the membrane, but others may be excluded.

A major determinant of diffusion in a biological system is membrane permeability. Small, uncharged molecules pass through cellular membranes easily, while most and/or charged molecules cannot pass through the membrane. The movement of water across a selectively permeable membrane, like the plasma membrane

1) The relationship between the rate of diffusion, volume and surface area is that the surface area of a subject somewhat correlates with the volume of the object. When the surface area and volume are set up as a ratio, this gives a good idea as to how fast the rate of diffusion will be. The higher surface area to volume ratio, the faster the rate of diffusion. This is because then there is more surfaces to diffuse through and there materials to diffuse, the rate of diffusion is a lot faster. Conversely, when there is a smaller surface area for more molecules to diffuse through, the rate of diffusion will be lower.

The difference is that along with large molecules, living cells prevent molecules with positive charges and solubility. This is not representing in dialysis tubing, and is only found in living cells because the tubing is only based on molecular size (98). When referring the rate of diffusion, the concentration gradient influences the diffusion rate, based on the factors of temperature. The ability for molecules diffuse from high to low concentrations primarily depends on the concentration gradient between the two areas.(96-99). My hypothesis for the study is that in the hypotonic, hypertonic, and isotonic solutions, the direction and rate of osmosis will determine based on the concentration inside the dialysis tubing. My prediction is that if the solution is hypotonic the results will decrease, if the solution is hypertonic the results will increase and if the solution is isotonic the solution will vary and or remain constant.

Which materials diffused from the left beaker to the right beaker? NaCl, Urea, and Glucose at MWCO 200

The major objective of the experiment was to test the effect of the concentration gradient on the diffusion rate. It was hypothesized that the greater the stronger the concentration gradient, the faster the rate of diffusion would be. To test this, dialysis tubes were submerged in different concentration fructose solutions. We weighed the tubes at specific time intervals to measure the rate of diffusion of water in each different solution. The results illustrated that increased concentration gradient increases the rate of diffusion of water in the tubes. We concluded that as concentration of the

Diffusion is the movement of molecules from a region of higher concentration to a region of lower concentration. The rate at which molecules diffuse can be determined by the relationship of molecular weight and that rate of diffusion through a membrane. Hypothesis of this experiment is that the fluid with higher molecular weight will diffuse at a slower rate and distance.

All cells contain membranes that are selectively permeable, allowing certain things to pass into and leave out of the cell. The process in which molecules of a substance move from an area of high concentration to areas of low concentration is called Diffusion. Whereas Osmosis is the process in which water crosses membranes from regions of high water concentration to areas with low water concentration. While molecules in diffusion move down a concentration gradient, molecules during osmosis both move down a concentration gradient as well as across it. Both diffusion, and osmosis are types of passive transport, which do not require help.

The beaker was then filled partially with distilled water; 1 ml of potassium iodide was then added, and the solution was tested for the presence of glucose. This data was recorded in table 1 on the data sheet along with the starting color of both the potassium iodide solution and the glucose/starch solution. The dialysis tubing was then submersed into the beaker containing the potassium iodide solution, and set aside for 30 minutes to allow maximum diffusion.