 # An Investigation to Find the Effect of Bile Salts of on the Digestion of Lipids

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AN INVESTIGATION TO FIND THE EFFECT OF BILE SALTS OF ON THE DIGESTION OF LIPIDS

I have investigated that if there is an effect of bile salts on the digestion of lipids in the milk. When lipids are broken down in to fatty acids and glycerol (see below), the acid lowers the pH of the mixture. To help me determine and measure the digestion of lipids, a pH indicator phenolphthalein has been used to measure the pH of the mixtures. The pH indicator will change the colour from pink to either white (if bile is not contained) or brown (if bile is contained), this will indicate that the solution is changing from alkaline to acidic. So if I want to use phenolphthalein, I will have to firstly make the mixtures alkaline. This will be achieved by
sx = ∑x2 – (∑x)2 n n – 1
I will draw a table in which I will work out ‘∑x2’ and ‘(∑x)2’. Treatment tube number Time/seconds
(x) x2
8 101 10201
9 98 9604
10 103 10609
∑x = 302 ∑x2 = 30414
(∑x)2 = 91204

The standard deviation for the y:
Sx = ∑x2 – (∑x)2 n n n – 1 = (30414– (91204 ÷ 3)) ÷ 2 = (30414– 30401.3*) ÷ 2 = 12.7 ÷ 2 = 6.35 The standard deviation for the y:
Sy = ∑y2 – (∑y)2 n n n – 1
I will draw a table in which I will work out ‘∑y2’ and ‘(∑y)2’. Treatment tube number Time/seconds
(y) y2
5 12 144
6 14 196
7 16 256
∑x = 42 ∑x2 = 596
(∑x)2 = 1764

Sy = ∑y2 – (∑y)2 n n n – 1 = (596– (1764 ÷ 3)) ÷ 2 = (596– 588) ÷ 2 = 8 ÷ 2 = 4 My null hypothesis is that bile salts have no effect on the digestion of lipid and lipase will still breakdown the fat, the reaction times will be the same.

The critical value of probability is 0.05. The critical value at ‘4’ degrees of freedom with probability ‘0.05’ is 2.776.

tcalc = x – y sx2 + sy2 n1