Using Binary Complex Assembly And Membrane Fusion By The Exocyst Protein Sec3

2884 WordsJan 27, 201512 Pages
Promoting t-SNARE Binary Complex Assembly and membrane Fusion by the Exocyst Protein Sec3 Peng Yue A DISSERTATION in Biology Presented to the Faculties of the University of Pennsylvania in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy 2015 Supervisor of Dissertation ______________________ Wei Guo, Professor of Biology Graduate Group Chairperson ______________________ Michael Lampson, Associate Professor of Biology Dissertation Committee Fevzi Daldal, Professor of Biology Tatyana Svitkina, Professor of Biology Erfei Bi, Professor of Cell and Developmental Biology Claudio G. Giradudo, Assistant Professor of Pathology and Laboratory Medicine James Shorter, Associate Professor of Biochemistry and Biophysics ACKNOWLEDGMENT (optional) ABSTRACT Promoting t-SNARE Binary Complex Assembly and membrane Fusion by the Exocyst Protein Sec3 Exocyst is an octameric protein complex that mediates the initial contact between secretory vesicles and the plasma membrane. The final fusion event is mediated by SNAREs (Soluble N-ethylmaleimide Sensitive Factor Attachment Protein Receptor), proteins residing on both vesicles and the PM. The assembly of the SNARE complex drives membrane fusion. SNARE mediated fusion at the plasma membrane in yeast begins with the formation of a binary t SNARE complex composed of Sso1/2 and Sec9 followed by its binding to the v SNARE protein Snc1/2. It was previously shown that SNARE assembly is
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