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1. Indicate the objective of this method. 2. Pick out the donor.. . of & the recipient.. 3. Why we qualify the obtained bacteria as transgenic? 4. Pick out the name of the factor that cuts both DNA........ 5. Pick out the factor that fuses the bacterial DNA with the human DNA....

1. Indicate the objective of this method. 2. Pick out the donor.. . of & the recipient.. 3. Why we qualify the obtained bacteria as transgenic? 4. Pick out the name of the factor that cuts both DNA........ 5. Pick out the factor that fuses the bacterial DNA with the human DNA....

Human Heredity: Principles and Issues (MindTap Course List)
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter8: The Structure, Replication, And Chromosomal Organization Of Dna
Section: Chapter Questions
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The whole mechanism is summarized by the 8 steps of the figure below: protein, we insert the DNA of the desired human protein in the bacterial DNA. Therefore, when bacteria produce its own proteins by transcription & translation processes, our desired protein fast with no expensive costs; the easier choice are bacteria. To allow the bacteria to produce the wanted We want to produce certain human protein to be used in therapies. To do so, we need a machine that multiplies is produced as well. Note that proteins can be hormones, antibodies, enzymes, antigens, .... Doc-1-Principles of Biotechnology BeChride 12 SE R Venn le Rad Mo Human DNA 2.DNA is cleaved by restriction enzyme to isolate the gene of interest to obtain the gene in interest 1. Extract Human Cell Bacterial Plasmid (Circular DNA) 5. Ligation (Fusion) between the linearized A O 3.Extract plasmid & the human gene by Ligase enzyme Bacteria 4. Plasmid is cleaved by the same restriction enzyme rDNA 7. Cultured in a nutritive medium to allow bacterial multiplication Recombinant Plasmid is obtained 6. Insert it back to the bacteria Transgenic Bacterium is obtained O 00 rDNA 8. Purifying the human protein in interest FDNA Culture Remark: Escherichia Coli is the most widely used bacterium in the biotechnological processes or gene cloning techniques because it has a short, well-known, easily manipulated genome and high rate of proliferation or multiplication. 1. Indicate the objective of this method. 2. Pick out the donor...... of the DNA. & the recipient... 3. Why we qualify the obtained bacteria as transgenic'? 4. Pick out the name of the factor that cuts both DNA. S. Pick out the factor that fuses the bacterial DNA with the human DNA. Doc-2- Insulin Biotechnology Doc-3- Vaccine Therapeutic Drugs Applications Doc-4- Antibodies Transgenic Organsims Transgenesis Ch-8
Transcribed Image Text:The whole mechanism is summarized by the 8 steps of the figure below: protein, we insert the DNA of the desired human protein in the bacterial DNA. Therefore, when bacteria produce its own proteins by transcription & translation processes, our desired protein fast with no expensive costs; the easier choice are bacteria. To allow the bacteria to produce the wanted We want to produce certain human protein to be used in therapies. To do so, we need a machine that multiplies is produced as well. Note that proteins can be hormones, antibodies, enzymes, antigens, .... Doc-1-Principles of Biotechnology BeChride 12 SE R Venn le Rad Mo Human DNA 2.DNA is cleaved by restriction enzyme to isolate the gene of interest to obtain the gene in interest 1. Extract Human Cell Bacterial Plasmid (Circular DNA) 5. Ligation (Fusion) between the linearized A O 3.Extract plasmid & the human gene by Ligase enzyme Bacteria 4. Plasmid is cleaved by the same restriction enzyme rDNA 7. Cultured in a nutritive medium to allow bacterial multiplication Recombinant Plasmid is obtained 6. Insert it back to the bacteria Transgenic Bacterium is obtained O 00 rDNA 8. Purifying the human protein in interest FDNA Culture Remark: Escherichia Coli is the most widely used bacterium in the biotechnological processes or gene cloning techniques because it has a short, well-known, easily manipulated genome and high rate of proliferation or multiplication. 1. Indicate the objective of this method. 2. Pick out the donor...... of the DNA. & the recipient... 3. Why we qualify the obtained bacteria as transgenic'? 4. Pick out the name of the factor that cuts both DNA. S. Pick out the factor that fuses the bacterial DNA with the human DNA. Doc-2- Insulin Biotechnology Doc-3- Vaccine Therapeutic Drugs Applications Doc-4- Antibodies Transgenic Organsims Transgenesis Ch-8
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