1. Indicate the objective of this method..... of the DNA 2. Pick out the donor...... & the recipient... 3. Why we qualify the obtained bacteria as transgenic?
Q: Give at least four(4) uses/application of PCR. Explain.
A: PCR (Polymerase Chain Reaction) is use to amplify DNA. It can make billions of copies of a target…
Q: Which of the following protocols involve any Immunological reaction? Oa. ELISA only Ob. Southern…
A: There are number of of methods and techniques developed to treat different kind of diseases and…
Q: DEFINE THE FOLLOWING: 1) restriction enzyme 2) plasmid 3) recombinant DNA
A: Ans 1) Restriction enzymes are enzymes that are isolated from bacteria that can cleave…
Q: Which of the delivery techniques mentioned in Table 2 can be used to best transfer recombinant…
A: * They are many techniques for delivering the recombinant plasmids into the recipient cells. *…
Q: ou followed all the steps correctly with a kit you bought last week. However, NO purified DNA came…
A: Plasmids exist naturally in bacterial cells . In some cases bacteria need to pick up plasmid from…
Q: The cath sample was both filtered to remove all bacteria, and then treated with DNase. Based on…
A: A cath or catheter is a thin hollow tube, which is a medical equipment that is used for various…
Q: How can the CRISPR bacterial immune system be used to specifically and accurately edit genomes in…
A: CRISPR is a family of DNA sequences found in the genomes of prokaryotic organisms such as bacteria…
Q: Explain The replica plating technique?
A: Replica plating is used in the microbiological experiment. It helps in the testing of various…
Q: frederic griffith used the word transformation to describe the changes in bacteria that he observed.…
A: Frederic Griffith was a British bacteriologist whose experiments proved the existence of a…
Q: Genetically identical organisms derived from a single genetic source are Select one: a. GMOs b.…
A: Genetic material is nothing but the sequence of nucleic acids which is called as DNA. It contains…
Q: What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a…
A: Recombinant DNA technology (rDNA technology) is very helpful for analysing the entire genome of a…
Q: What technique could be used to confirm the presence or absence of plasmids in the donor, recipient…
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Plasmids are the only vectors currently available for use in recombinant procedures.True or false?
A: The technology used for joining different DNA molecules from different species is known as…
Q: Transgenic microorganisms can be generated to produce human proteins to use as a therapeutic agent…
A: To design A project to generate bacterium that produces a human protein called ABC
Q: you transform bacteria with the pGLO plasmid. What do you need to add to the media to get them to…
A: Green Fluorescent Protein (GFP) is obtained from jelly fish (Aequorea victoria). It has excitation…
Q: Why doesn't the CRISPR system cut the bacterium's own DNA, at the point in its genome where it…
A: CRISPR is an acronym for Clustered Regularly Interspaced Short Palindromic Repeat. This name refers…
Q: Identify one specific example of how this process has been used 10. Using the example provided in…
A: Transgenic organisms are created by manipulating genes in the the plant of the animal species. Thud…
Q: If HIV was just an enveloped virus, but not a retrovirus, which of the following drugs would likely…
A: Virus captures the host replication machinery and multiplies its genome. This is the foundation of…
Q: .What is RT-PCR? 2.What is it used for? 3.Why do you think it is important in relation to the…
A: RT-PCR stands for Real-time Polymerase Chain reaction. It simply means that enzyme polymerase is…
Q: Discuss some advantages and disadvantages of bacteriophage therapyin treating bacterial infections.
A: Bacteriophage is defined as the type of virus that infects bacteria. It is also called as bacterial…
Q: Explain in your own words how bacteria are grown to form the sample for the antibiotic resistance…
A: Antibiotic resistance The term antibiotic resistance is made up of two words i.e. resistance(ability…
Q: Explain how PCR/OLA (polymerase chain reaction/oligonucleotide ligation assay) can be used in the…
A: Introduction Sickle cell disorder is a rare condition in which the red blood cells of the individual…
Q: State the differences between the plasmid in Figure 1 with pBR322 in terms of their sercening…
A: According to the question, we have to state the differences between the plasmid in figure 1 with…
Q: Give three reasons why Salmonella strains are very useful for mutagenesis studies
A: Ans- The standard set of bacterial strains that are used in the mutagenicity testing of…
Q: How does diagnostic method for detecting Covid-19 using RT-PCR work?
A: RT–PCR also called Real-time PCR (polymerase chain reaction) is a nuclear-derived method or test…
Q: What is the addition and limitation of the RT-PCR approach? Keep CoViD-19 in mind
A: Viruses are a nucleoprotein entity which is able to utilize the synthetic machinery of a living cell…
Q: In the bacterial transformation experiment, what is the primary purpose of using an…
A: Transformation experiment was given by Griffith. In bacteria, DNA can be transferred using…
Q: What is the nature of the primers used in RT-PCR tests? (i.e. are they DNA primers or RNA primers?)
A: In RT-PCR, the RNA sample obtained from a virus is converted into DNA, and the DNA is amplified and…
Q: _________1. Which is a transgenic organism? A bacterium that has received genes via conjugation. A…
A: Transgenic organisms An organism whose genetic characteristics have been altered by the Insertion…
Q: If a selection assay be made to identify cells that have incorporated the recombinant DNA, what type…
A: The incorporation of recombinant DNA into the bacterial cell is known as transformation. It leads to…
Q: 1. Indicate the objective of this method. 2. Pick out the donor.. . of & the recipient.. 3. Why we…
A: Deoxyribonucleic acid (DNA) stores the cell’s genetic information and is present in the nucleus of…
Q: 4th calculation: total amount (milligram) of DNA in 100g wheat germ assuming an extraction…
A: DNA extraction is a method of separating DNA from cell membranes, proteins, and other cellular…
Q: Based on the image below of a Kirby-Bauer assay, this bacteria is most susceptible to which…
A: Antibiotics are substances that have the ability to kill bacteria or restrict their growth. They are…
Q: What are the optimal conditions for EcoRV and Aval restriction enzymes?
A: Restriction enzymes are also known as molecular scissors since they are used in cutting specific…
Q: What is the value of antibiotics to public health, what is the harm to public health of antibiotic…
A: Antibiotics are also called antibacterial or antimicrobial drugs. They are used in the treatment and…
Q: Bacterial mutants can be classified on the basis of the method, Which is those methods?
A: Microorganisms undergo mutations under environmental stress that causes sudden heritable changes in…
Q: plasmid cloning vector
A: The term vector refers to the DNA molecules that act as transporting vehicle which carries target…
Q: escribe how the bacteria should have the ability to be recovered back from the infected host for the…
A: Bacteria are microscopic organisms which belong to prokaryote because these are unicellular…
Q: The purpose of using soap in the DNA extraction activity using frozen strawberries is to
A: A gene is an area of DNA molecule or polymer of amino acid that encodes function. A chromosome…
Q: Explain the purpose of the regulations on DNA technology. (Give 4 points)
A: Purpose Of The Regulation On DNA Technology --- Biotechnology -- Advancement in the technology is…
Q: Choices are in the picture. Choose the primary impact of the given agents to the genetic material.…
A: Heritable change in the genetic material that gives an altered form of a gene is known as mutation.…
Q: In the bacterial transformation experiment, what is the primary purpose of using an…
A: Bacteria are Prokaryotic microbes which undergo splitting process and form similar cells. Genetic…
Q: Explain how reverse-transcriptase PCR is carried out.
A: The reverse transcriptase-polymerase chain reaction is used to identify the genome of many RNA…
Q: Give 5 advantages and disadvantages of GMOs ( genetically modified organism ).
A: Genetically modified organisms or GMOs are organisms whose genetic material is altered either by…
Q: Recent scientific studies show that the plasmid plays a vital role in gene transfer not only in the…
A: A plasmid is circular, extrachromosomal, small piece of DNA present in the cytoplasm that is…
Q: Vhat is needed from the cells for PCR?
A: The polymerase chain reaction (PCR) is a technique for replicating a piece of DNA millions of times.…
Q: What is the point of doing a modified Furter-Meyer Test? What is the underlying idea of this test?…
A: Modified Furter-Meyer Test is used to detect the presence of alpha-tocopherols.
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- In generating mutations in a bacterial gene involved in antibiotic resistance, a number of point mutations are isolated that render the bacteria sensitive to the antibiotic. You would like to sequence the gene in order to characterize the mutations, but unfortunately, your lab partner just finished the last of the lab's supply of DNA polymerase. The only things at your disposal are materials for performing a western blot, allowing you to visualize the protein encoded by the gene. How would you identify which mutations are likely to be the result of a missense mutation, which are likely to be the result of a nonsense mutation, and which are likely to be the result of a frameshift mutation?A bioengineer takes the gene from the human liver cell and insert into a bacterial chromosome. Then, bacteria transcribe this gene into mRNA and translate this mRNA into protein. This protein produced is useless and has been found to contain more amino acid than the protein made by the eukaryotic cell. Please explain the reason for that issue.A bioengineer takes the gene from the human liver cell and insert into a bacterial chromosome. Then, bacteria transcribe this gene into mRNA and translates this mRNA into protein. This protein produced is useless and has been found to contain more amino acids than the protein made by the eukaryotic cell. Please explain the reason for that issue.
- During experimental RNAi, how does the researcher affect expression of a target gene? Group of answer choices Inject custom, lab-made double-stranded RNA into the specimen that will be targeted by the cell's existing Dicer to create siRNA complementary to the target gene's transcript. Inject lab-made Dicer proteins into the specimen which will cut up the target gene's transcript. Inject custom, lab-made siRNAs complementary to the target gene's transcript.In gene therapy, why can't a gene be inserted directly into the cell? It was mentioned on this website: https://ghr.nlm.nih.gov/primer/therapy/procedures and many others but there's no explanation for it.Our bodies are not defenseless against mutagens that alter our genomic DNA sequences. What mechanisms are used to repair DNA?
- A person’s 5’ capping machinery has failed, but the PolyA tail machinery is still working. Pretending that they actually survive this issue, which of the following is a possible consequence? The person acquires many somatic mutations. The person has higher than normal levels of mRNA in the nucleus. The person has dTTP bases in the mRNA instead of UTP. The person develops a fever and body aches. If not higher than normal levels of mRNA in the nucleus, would it be fever and body aches due to the immune system being launched because mRNA cannot recognize self?A faulty protein is found in the cell. A further investigation proved that the DNAsequence in the genome responsible for this protein was intact, there is no change insequence but still the protein is found as faulty. What do you think why the protein isproduced as faulty? Explain with logic.You construct a translational reporter gene fusion of your favourite gene. How can you assess whether the translational fusion is functionally active?
- The nontemplate strand of a gene reads 5' ATGCGCTAGCTG 3'. What is the sequence of the mRNA transcript that would be made in this region? Group of answer choices 5' AUGCGCUAGCUG 3' 5' CAGUAGCGCAU 3' 5' UACGCGAUCGAC 3' 5' CAGCTAGCGCAT 3' 5' ATGCGCTAGCTG 3'A section of template DNA has the following sequence of nitrogenous bases: 5’-CGATTACTG-3’. Which of the following sequences would be produced as a result of transcription? Group of answer choices CGTUUTCAG CGAUUACUG GCUAAUGAC GCTAATGACTransposase protein cana. bind to DNA.b. catalyze the excision of a transposable element froma donor site.c. catalyze the insertion of a transposable element intoa target site.d. All of the above