1. Make the following solutions: Lysis buffer (100 mL): 0.1 M NaH2PO4, 10 mM Tris, 6 M Urea, pH 8.0 Dissolve the bulk reagents, adjust the pH with HCI as necessary, dilute to 500 mL Bradford Dye (500mL): 0.01% (w/v) Coomassie Blue G-250, 5% (v/v) ethanol, 8.5% phosphoric acid Dissolve Coomassie in ethanol, then add the phosphoric acid. Dilute to 500 mL and filter through #1 Watman filter paper. Bovine Serum Albumin: 1 mg/mL in diH20

1. Make the following solutions: Lysis buffer (100 mL): 0.1 M NaH2PO4, 10 mM Tris, 6 M Urea, pH 8.0 Dissolve the bulk reagents, adjust the pH with HCI as necessary, dilute to 500 mL Bradford Dye (500mL): 0.01% (w/v) Coomassie Blue G-250, 5% (v/v) ethanol, 8.5% phosphoric acid Dissolve Coomassie in ethanol, then add the phosphoric acid. Dilute to 500 mL and filter through #1 Watman filter paper. Bovine Serum Albumin: 1 mg/mL in diH20

Chemical Principles in the Laboratory

11th Edition

ISBN:9781305264434

Author:Emil Slowinski, Wayne C. Wolsey, Robert Rossi

Publisher:Emil Slowinski, Wayne C. Wolsey, Robert Rossi

Chapter24: The Standardization Of A Basic Solution And The Determination Of The Molar Mass Of An Acid

Section: Chapter Questions

Problem 3ASA: A 0.3012g sample of an unknown monoprotic acid requires 24.13mL of 0.0944MNaOH for neutralization to...

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