1. Most pure proteins are insoluble in pure distilled water but dissolve in dilute salt concentrations. However, the addition of high concentrations of neutral salts to an aqueous solution of protein causes it to precipitate. Name this phenomenon and suggest a molecular explanation for the observation that high concentrations of added salts decrease the solubility of proteins. 2. Capillary gel electrophoresis (CGE) forms part of a family of related techniques that comprise capillary electrophoresis (CE). Give a brief description of this technique with particular reference to its separation characteristics and applications.
1. Most pure proteins are insoluble in pure distilled water but dissolve in dilute salt concentrations. However, the addition of high concentrations of neutral salts to an aqueous solution of protein causes it to precipitate. Name this phenomenon and suggest a molecular explanation for the observation that high concentrations of added salts decrease the solubility of proteins. 2. Capillary gel electrophoresis (CGE) forms part of a family of related techniques that comprise capillary electrophoresis (CE). Give a brief description of this technique with particular reference to its separation characteristics and applications.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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