1abc Biochemistry IChem 348Problem Set #1: Titrations, Buffers, and pKa's1. Tyrosine has pK, values of 2.2, 9.2 and 10.5.a. Draw the pH/structure number line: Show all of the structural protonated/deprotonated forms oftyrosine, indicate the net charge of each and the pH at which each species predominates.b. Most amino acids with a third pK, (for their R group) are categorized as a "charged" amino acid,such as His, Glu, Lys, Asp and Arg. Explain how tyrosine can have a third pK, not be classified ashaving a "charged" R group.c. Draw a titration curve for tyrosine, labeling the buffer regions and equivalence pointsd. circle the region on your titration curve where half the R groups are ionized.e. What is the isoelectric point for lysine? Indicate it on your titration curve with "pl"f. What is the approximate net charge of tyrosine at pH 7?2. You wish to make 1 L of a 100 mM Phosphate buffer at pH=7.0. You have each of the forms ofphosphate available: phosphoric acid (98 g/mol), monosodium phosphate (120 g/mol) and disodiumhydrogen phosphate (142 g/mol). The pK, values for phosphate are 2.12, 7.21, 12.67).3. Biomolecules come in all shapes and sizes and need to interact with one another,. Discuss the possiblechemical properties of the exterior of a protein that favorably interact with the following molecules. What arethe forces that are driving the interaction?a. A protein interacting with:a.OHH₂C-C-CH3Ó–CH3b. A protein interacting with the surface of a surface of a lipid bilayer, which is composed ofhydrocarbons in the interior and charged, polar head groups on the exterior.c. A protein interacting with lipids (such as the inside of a membrane)4. An enzyme catalyzed reaction was initiated in 1.0 mL of 0.06 M HEPES buffer, pH 7.55. During thecourse of the reaction 3.0 µmol of H" was produced.What was the final pH of the reaction mixture, assuming the enzyme and reaction metabolitesother than H+ did not influence it? The pKa for HEPES is 7.55.b. What would the pH be in the absence of buffer (If the reaction occurred in water)?

Tyrosine have 3 ionizable groups. An alpha carboxylic acid group, an alpha amino group and a…

1. Tyrosine has pK, values of 2.2, 9.2 and 10.5. a. Draw the pH/structure number line: Show all of the structural protonated/deprotonated forms of tyrosine, indicate the net charge of each and the pH at which each species predominates. b. Most amino acids with a third pK, (for their R group) are categorized as a "charged" amino acid, such as His, Glu, Lys, Asp and Arg. Explain how tyrosine can have a third pK, not be classified as having a "charged" R group. c. Draw a titration curve for tyrosine, labeling the buffer regions and equivalence points

1. Tyrosine has pK, values of 2.2, 9.2 and 10.5. a. Draw the pH/structure number line: Show all of the structural protonated/deprotonated forms of tyrosine, indicate the net charge of each and the pH at which each species predominates. b. Most amino acids with a third pK, (for their R group) are categorized as a "charged" amino acid, such as His, Glu, Lys, Asp and Arg. Explain how tyrosine can have a third pK, not be classified as having a "charged" R group. c. Draw a titration curve for tyrosine, labeling the buffer regions and equivalence points

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter3: The Chemistry Of Life: Organic Compounds

Section: Chapter Questions

Problem 2TYU: VISUALIZE The structures depicted are (a) enantiomers (b) different views of the same molecule (c)...

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