1. What is the principle behind the DNA extraction? 2. Describe the features isolated DNA of banana.
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1. What is the principle behind the DNA extraction?
2. Describe the features isolated DNA of banana.
Step by step
Solved in 5 steps
- 1. What is/are the purpose(s) of DNA extraction? 2. What is the function of each reagent used in the extraction of DNA from a banana? a. Saltwater b. Liquid dishwashing soap c. Cold isopropyl alcoholMatch the method with the appropriate enzyme. _____ PCR a. Taq polymerase _____ cutting DNA b. DNA ligase _____ cDNA synthesis c. reverse transcriptase _____ DNA sequencing d. restriction enzyme _____ pasting DNA e. DNA polymerase (not Taq)After a polymerase chain reaction (PCR), agarose gel electrophoresis is often used to: a. amplify the DNA. b. convert cDNA into genomic DNA. c. convert cDNA into messenger RNA. d. verify that the desired DNA sequence has been amplified. e. synthesize primer DNA molecules.
- 2. Compare the homebased DNA extraction method with the Phenol:Chloroform extraction method.1. What are the considerations we need to consider in introducing recombinant DNA in plant calli and in animal cell lines? (Give at least three considerations) Explain and answer this comprehensively.1. Describe the features of isolated DNA of banana?
- 1.Briefly explain the rationales of adding chemicals that can affect DNA stability in a polymerase chain reaction (PCR). 2.Why DNA melting is required in PCR? Briefly explain how PCR can be used to detect DNA mutation.1. If you forgot to add nucleotides to your PCR master mix, which control reaction tube would have results that are different than they would be if you had prepared them correctly? 7. If you found that there was DNA amplification in your negative control tube, what could be an explanation for that result?1. What are the reaction components, and what equipment do you need for PCR?2. What can be possible source of DNA for PCR?3. What are some uses for PCR?
