2. The molar absorptivity of compound A & B were measured with pure solutions as follows: wavelength (nm) ɛa (M'cm-l) 2139 EB (M-'cm-') 8469 312 417 10892 4325 (a) Calculate the frequency at wavelength of 312 nm and 417 nm. What wavelength has the greatest frequency? What wavelength has the greatest energy (show your calculation)? (b) A mixture of compound A and B in a 1.00 cm cell had an absorbance of 0.543 at 312 nm and 0.762 at 417 nm. Find the concentrations of A and B in the sample.

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter14: Applications Of Ultraviolet-visible Molecular Absorption Spectrometry
Section: Chapter Questions
Problem 14.9QAP
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2. The molar absorptivity of compound A & B were measured with pure solutions as follows:
wavelength (nm)
Ea (M'cm-')
2139
EB (M-'cm-')
8469
312
417
10892
4325
(a) Calculate the frequency at wavelength of 312 nm and 417 nm. What wavelength has the
greatest frequency? What wavelength has the greatest energy (show your calculation)?
(b) A mixture of compound A and B in a 1.00 cm cell had an absorbance of 0.543 at 312
nm and 0.762 at 417 nm. Find the concentrations of A and B in the sample.
3. Given the molar absorptivity of a pH indicator at 525 nm is 16884 M-'cm for In and
1125 M-'cm- for HIn, calculate the pKa of the indicator if the absorbance of a 8.40 X 105
M solution of the indicator at pH 5.48 is 0.412 using a 1.00 cm cell.
Transcribed Image Text:2. The molar absorptivity of compound A & B were measured with pure solutions as follows: wavelength (nm) Ea (M'cm-') 2139 EB (M-'cm-') 8469 312 417 10892 4325 (a) Calculate the frequency at wavelength of 312 nm and 417 nm. What wavelength has the greatest frequency? What wavelength has the greatest energy (show your calculation)? (b) A mixture of compound A and B in a 1.00 cm cell had an absorbance of 0.543 at 312 nm and 0.762 at 417 nm. Find the concentrations of A and B in the sample. 3. Given the molar absorptivity of a pH indicator at 525 nm is 16884 M-'cm for In and 1125 M-'cm- for HIn, calculate the pKa of the indicator if the absorbance of a 8.40 X 105 M solution of the indicator at pH 5.48 is 0.412 using a 1.00 cm cell.
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