Below is a trace from an analytical gel-filtration column upon which a single sample containing a mixture of two proteins was loaded. - 230nm 800 MicA:ClpC 600 - 400 - ClpC 200 - MicA 20 40 60 80 100 120 140 Volume (mL) Normalized absorbance mAU
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What is happening in this trace? What do the peaks represent? Why is absorbance measured at 230 nm?
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- Construct a standard curve (absorbance versus protein concentration in the standards) Conc. (mg/ml) 0 0.25 0.5 0.75 1.0 Absorbance 0.000 0.126 0.249 0.361 0.464Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…The physician has ordered 500,00 units of IV Pfizerpen every 4 hours. How much diluent will you add for a concentration of 500,000 units/mL? How many milliliters will you administer?
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