5 B-Glucose can be converted to D-ribose through the pentose phosphate pathway, shown below. 2- O,PO pentose phosphate pathway 2- O,PO HO OH но он (a) What byproduct (not shown) is also formed in this reaction? (b) The key step in this pathway is where the six carbon chain is shortened to five carbons. 2- но 2- O,PO- NADP O,PO- HOR HO- но он Which structure below is an intermediate in this reaction? 容 но HO O,PO 2- O,PO- OH O,PO- O,PO- HOT FOPO, но но B D
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- 1. For a saturated fatty acid with 28 carbon atoms: a. Write the products obtained from one cycle of beta oxidation. b. How many cycles of beta oxidation will be required for its complete breakdown? How many Acetyl CoA will be produced after its complete breakdown? (show the calculation) c. Calculate the total number of ATP molecules generated (total energy yield) after complete oxidation of this fatty acid. (show the complete Stepwise calculation)1. Assuming that everything that’s needed to make tripalmitin comes from glucose, how many glucose would be used by adipose tissue in the synthesis of 1 mol tripalmitin? 2. How many ATP would be used (net of produced and used)? 3. If an animal absorbs 35 g glucose (MW = 180 g/mol) from drinking a can of pop, how many grams of tripalmitin (MW = 807 g/mol) can be produced from it in adipose tissue? Please provide only typed answer solution no handwritten solution needed allowedConsider docosanoic acid C12H43CO2H a. Label the alpha and beta Carbons. Show the beta-oxidation in an EXPANDED structure. b. Draw each acyl CoA derived from this fatty acid. c. How many acetyl Co A molecules are formed by complete beta-oxidation? d. How many cycles of beta-oxidation are needed for complete oxidation? e. How many molecules of ATP are formed from the complete catabolism of this fatty acid? Show the complete computation. f. How many moles of ATP per gram of fatty acid is formed from the complete catabolism of the given fatty acid? g. What is the molar mass of the given fatty acid? Solution: Show here the complete computations, [from a to e]
- Which carbon(s) of glucose could be labelled with carbon-14 label in order to have the label end up at carbon-2 in 3-phosphoglycerate during glycolysis? Justify your answer using structures. Since this question is for practice, show ALL STRUCTURES involved from glucose to 3-phosphoglycerate, inclusive. No need to provide mechanisms; cofactors; names; etc. but please show the arrows appropriately as being an equilibrium or irreversible (one-way or two-way arrow). If necessary one- or two-word explanations can be includedIn metabolism, glucose-6-phosphate (G6P)can be used for glycogen synthesis or for glycolysis, among otherfates. What does it cost, in terms of ATP equivalents, to storeG6P as glycogen, rather than to use it for energy in glycolysis?Hint: The branched structure of glycogen leads to 90% of glucose residues being released as glucose-1-phosphate and 10%as glucose.All are single-electron carriers EXCEPT: a. UQH2. b. Cyt bL. c. Cyt bH. d. Rieske protein Fe-S clusters. e. Cyt c1. 2.The energy input for the synthesis of glycogen occurs during which of the following enzyme catalysed steps? a. phosphoglucomutase b. UDP-glucose pyrophTosphorylase c. glycogen synthase d. amylo-(1,4®1,6)-transglycosylase e. none of the above require energy input ______________________________________________________________________________(
- 1. Give TWO (2) examples of sterols responsible for the synthesis of Vitamin D. How these sterols can be converted into Vitamin D? 2. Geranylgeranyl pyrophosphate (GGPP) is a universal precursor for all diterpene/diterpenoids. How GGPP is converted to linear, monocyclic, bicyclic and tricyclic diterpene/diterpenoids? Explain.1) Humans do not undergo net synthesis of carbohydrate from acetyl- CoA, yet carbons of acetyl -CoA can be incorporated into glucose and amino acids. Show pathways as to how this can happen. 2) A molecule -X inhibits oxidation of stearoyl-CoA by isolated mitochondria but has no effect on palmitoyl-carnitine oxidation, explain.4. Fatty acid COA esters are unable to diffuse through the inner mitochondrial membrane. Thus, transport through the inner membrane is cataylyzed by , a separate a two enzymes carnitine acyltransferase 1, and carnitine acyltransferase II? Why do we need two enzymes and not only one.
- 1. Acid phosphatases are an important group of enzymes that can be detected in human bloodserum. Under slightly acidic conditions (pH 5.0), this group of enzymes can hydrolyzebiological phosphate esters as follows:R-O-P-O3-2 + H 2O R-OH + HO-P-O3-2.Acid phosphatases are produced and can be detected in erythrocytes, kidney, spleen, the liver,and prostrate gland. The enzyme from the prostrate gland is clinically important because anincreased activity in the blood is frequently an indication of cancer of the prostrate gland.Tartrate ion can strongly inhibit the phosphatase from the prostrate gland, but not acidphosphatases from other tissues. How can you use the information above to develop a specificprocedure for measuring the activity of the acid phosphatase of the prostrate gland in humanblood serum?In the first cycle of fatty acid synthesis via the acyl carrier protein system, the acetyl group on fatty acid synthase condenses with the malonyl-ACP complex. Draw the structure of the products of this reaction. Respresent ACP by R1 do not include free synthase Be sure to include charges on atoms at Ph=7Starch synthase in plants contains a single active site that accommodatesADP–glucose. However, cellulose synthase may contain two UDP–glucose-binding active sites. Given the structural diff erences between starch and cellulose, explain why two substrate-binding sites would promote effi cient cellulose synthesis.