5. What is the equation of the standard curve 6. What is the concentration of amylase in the sample 7. Explain briefly the principle in the Bradford assay.

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter1: The Clinical Laboratory
Section1.8: Laboratory Math And Reagent Preparation
Problem 14RQ
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5. What is the equation of the standard curve
6. What is the concentration of amylase in the sample
7. Explain briefly the principle in the Bradford assay.
Transcribed Image Text:5. What is the equation of the standard curve 6. What is the concentration of amylase in the sample 7. Explain briefly the principle in the Bradford assay.
BSA standard Bradford
(uL)
Distilled
Average
Tube
water (uL)
reagent (mL) Absorbance
1
500
0.00700
2
490
10
5.
0.15720
480
20
5.
0.35035
4
470
30
0.50745
460
40
5
0.71540
450
50
5
0.89290
Sample
300
0.24201
5.
3.
Transcribed Image Text:BSA standard Bradford (uL) Distilled Average Tube water (uL) reagent (mL) Absorbance 1 500 0.00700 2 490 10 5. 0.15720 480 20 5. 0.35035 4 470 30 0.50745 460 40 5 0.71540 450 50 5 0.89290 Sample 300 0.24201 5. 3.
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