7. Based on the information provided, how many "cuts" would you expected after digesting the ligated pCR cloning vector with EcoRI? Wells A F B.

Q: 6. Which of the following steps are catalyzed by Taq DNA polymerase in a PCR reaction? a.…

A: Polymerase chain reaction can be described as the molecular technique to rapidly synthesize millions…

Q: 2. a. Research DNA quantitation by UV absorption. Explain what A230, A260 and A280 values represent.…

A: Every biomolecule have an unique absorbance curve  of their own. This simply means that, the…

Q: What would be the expected effect on the PCR reaction, if you increased the temperature of the…

A: The polymerase chain reaction (PCR) is a technique for amplifying DNA sequences in the laboratory.…

Q: 3. What is in the master mix and why do you need each component? 4. Why do you need to perform PCR…

A: Polymerase chain reaction (PCR) is a molecular biology technique that is used for amplifying the…

Q: 1.a. Match the plasmid illustration to its gel lane. 1.b What is the size of the longest DNA…

A: The gel electrophoresis is the process that is routinely used in laboratories two separate different…

Q: D. PCR of lacZ GENE 1. You will be preparing a PCR reaction for your "G" and "L" tubes. When…

A: PCR (Polymerase chain reaction) is a scientific technique for creating numerous copies of a DNA…

Q: 33 - The correct procedures for gene cloning are given as a) PCR/transformation/ligation/restriction…

A: Gene cloning is the method of production of numerous copies of a gene fragment.

Q: 6. Describe the process of DNA sequence of interest detection, using these term appropriately,…

A: *DNA sequencing is a technique used to find the exact sequence of bases like Adenine, Guanine…

Q: Explain the importance of different temperatures in PCR. Tm of forward primer= 62 °C Tm of reverse…

A: Polymerase chain reactions are the technique used to make millions of copies rapidly of a DNA…

Q: 7. Which primer pair would w getting a single PCR band w 1 4 4 5' B 3' A a 1 and 2 b. 1 and 3 c. 2…

A: In the polymerase chain reaction (PCR), a primer is a brief, single-stranded DNA sequence. A couple…

Q: 2. What are restriction digestion and gel electrophoresis used for in this cloning series of…

A: Restriction digestion technique is implicated in biotechnology or related field during cloning…

Q: 4. Write the Edman degradation technique used for the sequencing of proteins

A: “Since you have asked multiple questions, we will solve the first question for you. If youwant any…

Q: What characteristics of VNTR and STR make them useful for DNA fingerprinting?

A: According to guidelines we have to answer the first question only. so please kindly post the…

Q: 3A.Below are a few (of the many) possible errors that could adversely affect PCR or analysis of PCR…

A: A PCR result can be disappointing in many ways. If DNA and primer concentration are reversed, the…

Q: 3. Give a schematic diagram of the different steps involved in PCR (Polymerised chain reaction) of a…

A:

Q: 7. Fill in the blank. In the picture below, the oldest spacer that has been incorporated in the…

A: CRISPR is a group of DNA sequences that are found in the genomes of prokaryotic organisms like…

Q: following site does the restriction enzymes act? 32. Restriction Fragment Length Polymorphism (RFLP)…

A: Restriction endonucleases are enzymes that cuts the DNA at specific DNA sequence known as…

Q: What is the difference between an antigen test and a PCR test?          a. What are the benefits…

A: PCR- PCR stands for polymerase chain reaction. Antigen test- Antigen tests are immuno-assays which…

Q: С 17 Based on the knowledge you gained from the cloning module, which of the bands in the figure is…

A: Agarose gel electrophoresis is a method used in molecular biology to resolve DNA fragments on the…

Q: 6. Why do you think the Ames test is preferable to the use of animal models to screen chemical…

A: Ames test is used to find whether a given chemical causes mutation in the DNA in the animal models.…

Q: 1. If you forgot to add nucleotides to your PCR master mix, which control reaction tube would have…

A: PCR is used for the amplification of molecule of DNA.

Q: 1. write a precise and accurate differential report on the sequencing techniques.

A: Introduction The process of sequencing is the determination of nucleic acid sequence or the order of…

Q: 5) Which of the following is not a component of a PCR reaction mixture? A) DNA template B)…

A: Polymerase chain reaction (PCR) is a widely used method in molecular biology that is used to make…

Q: You are setting up your PCR reaction and accidentally add twice as much of the salt buffer as you…

A: Answer: PCR = Polymerase Chain Reaction is the technique used to amplify the strand of DNA using…

Q: 1. Use the plasmid shown to complete the table. # of Fragment Sizes Restriction Enzyme Used…

A: We’ll answer the first question since the exact one wasn’t specified. Please submit a new question…

Q: 3. (4 points) The DNA polymerase used in PCR is from a special archaebacteria called Thermus…

A: Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase. It is named after Thermus…

Q: 2.21 The two allelic fragments of DNA shown belowa (d) After 30 cycles of O (b) Diagram the gel…

A: PCR or polymerase chain reaction is a rapid and versatile in vitro technique for amplification of…

Q: 1. What does PCR stand for? 2. Explain the main objective of the PCR technique.

A: PCR It is a technique used in molecular biology which was developed by Kary Mullis, an American…

Q: 1. What is the role of salt and dishwashing liquid in the extraction/isolation of DNA from yeast and…

A: "DNA extraction  or DNA isolation" is a procedure or a method wherein  DNA is purified by physical…

Q: state why simplicity and speed are top priorities in selecting DNA isolation method

A: * Deoxyribonucleic acid that is isolation is the extraction process of DNA from other sources. * The…

Q: 1. The polymerase chain reaction (PCR) is used by scientists to amplify DNA, particularly when the…

A: We are answering 1st question only. For the rest of the questions please repost. The polymerase…

Q: Can you please answer this question

A: Taq polymerase DNA is obtained from a bacterium T. aquaticus. This bacterium is specifically found…

Q: 1. The final amount of each primer required in a PCR reaction is 0.025 nanomol. If the total volume…

A: Introduction  Using the formula of comparison,   n1V1= n2V2, we can calculate the volume of stock…

Q: 4. Create a PCR master mix for the following: 48 samples, 25 uL total volume per reaction, 2 uL…

A: PCR, or the polymerase chain reaction, is a chemical reaction that is used by molecular biologists…

Q: What extra step need to be taken after collecting samples of many viruses before PCR can be…

A: The PCR i.e. polymerase chain reaction is performed in 3 basic steps: Collection of samples…

Q: 8. Describe the possible outcome of a PCR experiment in which (a) one of the primers is…

A: Answer: Introduction: The polymerase chain reaction (PCR) technique, described by Kary Mullis in…

Q: 9. Five DNA samples are shown along with their recognition sites where a particular restriction…

A: Gel electrophoresis Gel electrophoresis is a molecular technique that involves the separation of DNA…

Q: 1. a. Draw the primers for this longer strand (consider optimal length):…

A: Introduction  PCR is the revolutionized technique invented by Karry Mullis in 1983, for which he got…

Q: 1. The following gel was produced in manual DNA sequencing. What would the unknown DNA template be…

A: The method of determining the nucleic acid sequence – the order of nucleotides in DNA – is known as…

Q: How is a randomized DNA library created?   MARK ALL THAT APPLY. the use of Mn2+ rather than Mg2+ by…

A: DNA libraries are a collection of a large number of various genes of choice kept or stored together…

Q: 2. Agarose gel clectrophoresis of PCR products from samples taken from a healthy, a carrier, and an…

A: Mutations are sudden and abrupt changes in the DNA, which can potentially change the cell process.…

Q: 1. Primers come at a concentration of 200 pmol//ul (uM). You want the final concentration of your…

A: Molarity One of the most popular measures used to quantify the concentration of a solution is…

Q: 1) Describe the ingredients of a PCR reaction.  List different temperatures used in each cycle and…

A: Answer:- Template DNA, PCR primers - a starting nucleotides sequence pre-requisite for the…

Q: 7:12 What is not true for Sequence tagged site (STS) markers: O cannot be mapped by fluorescence in…

A: Any sequence of DNA which shows polymorphism and can be recognized utilizing a molecular method is…

Q: 1. List all the differences between the DNA Polymerase Reaction and Polymerase Chain Reaction (PCR)?

A: The flow of genetic information from DNA to RNA, RNA to protein synthesis is called central dogma.…

Q: 1. The gel presented here shows the pattern of bands of fragments produced with several restriction…

A: The various fragments obtained in the gel are: Aatl 1 → 9kb, 3kb = 12kb.Ncol → 8kb, 4kb.Aatl 1 +…

Q: Why is each of the following reagents required to be in the PCR reaction tube?  Primers, Taq DNA…

A: Polymerase chain reaction is used to amplify a piece of DNA manifold. The technique can produce a…

Q: 2) Why was there a greater A260 absorbance reading for your DNA sample that was incubated at higher…

A: 2. Why was there a greater A260 absorbance reading for your DNA sample that was incubated at higher…

Q: 1.) What are the different temperatures used in PCR (polymerase chain reaction)?   What happens at…

A: Polymerase Chain Reaction or PCR is a technique that used for making copies or amplify  small…

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.