2.21 The two allelic fragments of DNA shown belowa(d) After 30 cycles of O(b) Diagram the gel pattern you would expect toamplified by PCR, and the products digested withd St (a) What will the total sizes of the two PCRareJorXBAboth PstI and Hind3.products be?from the doubly digested DNA.

PCR or polymerase chain reaction is a rapid and versatile in vitro technique for amplification of…

Answered: 2.21 The two allelic fragments of DNA…

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 12TYK

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2c) If the whole potoroo genome is 4.2 x 10' bp, and the highlyrepetitive DNA in the potoroo genome is composed entirely ofcopies of the sequence 5'AAGACT' and its complement, howmany copies of this sequence are present in the potoroogenome?
Heteroduplex DNA Formation in Recombination From the information in Figures 28.17 and 28.18, diagram the recombinational event leading to the formation of a heteroduplex DNA region within a bacteriophage chromosome.
Figure 9.10 You isolate a cell strain in which the joining together of Okazaki fragments is impaired and suspect that a mutation has occurred in an enzyme found at the replication fork. Which enzyme is most likely to be mutated?
When Chargaffwas performing his experiments, the tetranucleotide hypothesis, which stated that DNA was composed of GACT nucleotide repeats, was the most widely accepted view of DNA’s composition. How did Chargaff disprove this hypothesis?
Homologous Recombination, Heteroduplex DNA, and Mismatch Repair Homologous recombination in E. coli leads to the formation of regions of heteroduplex DNA. By definition, such regions contain mismatched bases. Why doesnâ€™t the mismatch repair system of E. coli eliminate these mismatches?
Why would an individual with a mutation that prevented the formation of recombination nodules be considered less fit than other members of its species?
2. The so-called hypervariable regions (HV1 and HV2)of the human mitochondrial genome are sometimesused in forensic analysis. They are two noncoding regions of the mitochondrial genome, each approximately 300 bp, that flank the origin of replication; thefunction of these DNA sequences is not well understood. However, these two regions of mtDNA showthe most variation (SNPs and InDels) among differentpeople. The DNA within HV1 and HV2 accumulatesmutations at ten times the rate of DNA sequences inthe nuclear genome.a. Under what circumstances would human mtDNAbe preferable over nuclear DNA for identifyingindividuals?b. What are the disadvantages of using mtDNA,relative to nuclear DNA, in order to identifyindividuals?
Although DNA polymerases require both a template and a primer, the following single-stranded polynucleotide was found to serve as a substrate for DNA polymerase in the absence of any additional DNA.3′ HO-ATGGGCTCATAGCCGGAGCCCTAACCGTAGACCACGAATAGCATTAGG-p 5′What is the structure of the product of this reaction?
1c) Even in automated sequencing, where you can include all 4 ddNTPs in one reaction, you need to include "normal" dNTPs as well as the ddNTPs - why are these necessary? Why can't you just put in the ddNTPs, since you've got all four of them available to the DNA polymerase?
6. a.) Which part (sugar, phosphate, or nitrogenous base) of the four types of nucleotides differ? b.) Based on the complementary base pairing rules we know that: A(denosine) pairs with _________ , and that G(uanine) pairs with _________.
(a) What two enthalpic factors stabilize DNA in double-helical form at lowtemperature?(b) What entropic factor destabilizes helical DNA at high temperature?(c) Why is the double-helical structure of DNA stabilized at moderate tohigh ionic strength?
2b) If you denatured the random 1000 bp fragments of potoroo DNA by heating them to 95°C. and then cooled them down to 60°C and allowed them to reanneal, you would find that approximately 20% of the DNA fragments renatured very rapidly, another 30% of the DNAfragments renatured moderately rapidly, and the remaining DNA fragments renatured relatively slowly. From these results, whatfraction of the potoroo genome is composed of highly repetitiveDNA? helpful Information

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2.21 The two allelic fragments of DNA shown belowa (d) After 30 cycles of O (b) Diagram the gel pattern you would expect to amplified by PCR, and the products digested with d St (a) What will the total sizes of the two PCR are JorX BA both PstI and Hind3. products be? from the doubly digested DNA.