a CG base pair, an adenine tautomer is placed across from the cytosine during replication b. in a T:A base pair, the adenine undergoes tautomerization prior to replication C. In a T:A base pair, S-bromouracil is incorporated across from an adenine prior to replication d. In an A:T base pair, oxoG is placed across from the A during replication Why is MutT so important? What occurs in cells lacking this enzyme? 33. How does DNA polymerase proofreading specifically recognize mispairs (by what mechanism)? 4. Define the roles of each in mismatch repair: MutS, Mutl, and MutH, exonuclease 3. How does E. coli distinguish between the old and new strand for mismatch repair? 36. How do human cells distinguish between the old and new strand for mismatch repair? es 37. What are the steps of base excision repair? Where does the glycosylase cleave? 38. Why does the OxoG failsafe system lead to more ATAD CG than CGD AT transversions? 3. what are the steps of nucleotide excision repair in bacteria? Why are the bacterial proteins in this pathway named "UV" and human proteins "XP"? *0. which repair system is tightly coupled to transcription? Why is it important to repair damage sensed by RNA polymerase quickly? *1. What is translesion DNA synthesis and when is it used? Why is it a last resort? Does it repair ssDNA or dsDNA? 42. What are the steps of homologous recombination repair? Which proteins are involved in searching for homologous sequences and how do they function? 43. What are the steps of nonhomologous end joining? 44. When is NHEJ used as a mechanism for DNA repair? Why is it a last resort? Does it repair ssDNA or dsDNA? 45. Describe the general steps needed for a cell to become cancerous. 46. How do mutations and chromosomal abnormalities affect proteins and lead to disease? 47. What role do cell cycle checkpoints have in cancer? 48. Define a tumor suppressor and oncogepe and give ap example of each. cogené 49. Define the role of ATM in the cell and cancer. Upon DNA damage, ATM decideş whether to activațe HRR or NHĖJ. Which is preferred and why?

a CG base pair, an adenine tautomer is placed across from the cytosine during replication b. in a T:A base pair, the adenine undergoes tautomerization prior to replication C. In a T:A base pair, S-bromouracil is incorporated across from an adenine prior to replication d. In an A:T base pair, oxoG is placed across from the A during replication Why is MutT so important? What occurs in cells lacking this enzyme? 33. How does DNA polymerase proofreading specifically recognize mispairs (by what mechanism)? 4. Define the roles of each in mismatch repair: MutS, Mutl, and MutH, exonuclease 3. How does E. coli distinguish between the old and new strand for mismatch repair? 36. How do human cells distinguish between the old and new strand for mismatch repair? es 37. What are the steps of base excision repair? Where does the glycosylase cleave? 38. Why does the OxoG failsafe system lead to more ATAD CG than CGD AT transversions? 3. what are the steps of nucleotide excision repair in bacteria? Why are the bacterial proteins in this pathway named "UV" and human proteins "XP"? 0. which repair system is tightly coupled to transcription? Why is it important to repair damage sensed by RNA polymerase quickly? 1. What is translesion DNA synthesis and when is it used? Why is it a last resort? Does it repair ssDNA or dsDNA? 42. What are the steps of homologous recombination repair? Which proteins are involved in searching for homologous sequences and how do they function? 43. What are the steps of nonhomologous end joining? 44. When is NHEJ used as a mechanism for DNA repair? Why is it a last resort? Does it repair ssDNA or dsDNA? 45. Describe the general steps needed for a cell to become cancerous. 46. How do mutations and chromosomal abnormalities affect proteins and lead to disease? 47. What role do cell cycle checkpoints have in cancer? 48. Define a tumor suppressor and oncogepe and give ap example of each. cogené 49. Define the role of ATM in the cell and cancer. Upon DNA damage, ATM decideş whether to activațe HRR or NHĖJ. Which is preferred and why?

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 22P

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