A sample containing histidine (pKas 1.8, 6.0, 9.3), lysine (pKas 2.2, 9.1, 10.5), and leucine (pKas 2.3, 9.7) at pH 7 is loaded onto an SP-Sepharose resin and then eluted at pH 7 using a gradient of increasing NaCl concentration. What would be the order of elution of the amino acids from the column (first to last)?

A sample containing histidine (pKas 1.8, 6.0, 9.3), lysine (pKas 2.2, 9.1, 10.5), and leucine (pKas 2.3, 9.7) at pH 7 is loaded onto an SP-Sepharose resin and then eluted at pH 7 using a gradient of increasing NaCl concentration. What would be the order of elution of the amino acids from the column (first to last)?

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter28: Atomic Spectroscopy

Section: Chapter Questions

Problem 28.13QAP

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