A. Differentiate the chemistry and function of primary and secondary antibodies in ELISA. B. Discuss the purpose of adding a blocking buffer. What happens when you fail to add the blocking buffer? Why do we use BSA as the primary component of the blocking buffer?
A. Differentiate the chemistry and function of primary and secondary antibodies in ELISA. B. Discuss the purpose of adding a blocking buffer. What happens when you fail to add the blocking buffer? Why do we use BSA as the primary component of the blocking buffer?
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter4: Basic Immunology And Immunohematology
Section4.7: Rh Typing
Problem 2RQ
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A. Differentiate the chemistry and function of primary and secondary antibodies in ELISA.
B. Discuss the purpose of adding a blocking buffer.
- What happens when you fail to add the blocking buffer?
- Why do we use BSA as the primary component of the blocking buffer?
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