After using the blender to blend the split peas, water, and salt mixture, cheesecloth is used to. . O a. Filtrates the contents of the blender to collect the strained fluid for DNA extraction O b. Precipitate the nucleic acid Oc. Separate the DNA from the protein that folded and protected the DNA O d. Collect pure nucleic acid O e. Filtrate the mixture and precipitate the protein

After using the blender to blend the split peas, water, and salt mixture, cheesecloth is used to. . O a. Filtrates the contents of the blender to collect the strained fluid for DNA extraction O b. Precipitate the nucleic acid Oc. Separate the DNA from the protein that folded and protected the DNA O d. Collect pure nucleic acid O e. Filtrate the mixture and precipitate the protein

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 2RQ: n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules...

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Place the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.
A) Which molecule in the gel is the smallest - A, B, C, or D? Explain how you determined this. B) Identify one lane in this gel (1, 2, 3, 4, 5, 6, or 7) that shows evidence of DNase treatment. Explain how you determined this.
you performed a sanger sequencing on a particular segment of DNA. Explain at the molecular level what your gel profile would look like due to this mistake. a. you forgot to add ddNTPs to your test tube. b. you added too much ddNTPs to your test tube
Why must the lagging strand of DNA be replicated in short pieces a. Because of limited space b. To make proofreading of code easier . C. Otherwise, the helix will become distorted . D. The DNA polymerase can synthesize in only one direction
n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules _______. a. are from different organisms b. have different lengths c. have different nucleotide compositions d. have different genes
When working with the gel electrophoresis chamber, what must we keep in mind? A. Keeping the gel flat on the table without any buffer solution B. Not changing your pipette in between DNA samples C. Not shaking the chamber after the DNA has been loaded D. Loading two DNA samples per a single well
A. A plasmid is shown with the locations of various restriction enzyme sites labeled. If you cut the plasmid with Xhol and Xbal, which lane of the agarose gel represents the DNA fragments you would expect from the digestion? B. If you now decide to cut the plasmid with EcoRI, how many fragments will be produced and what will their sizes be? C. When running DNA samples on agarose gel, an electric field is applied. Towards which electrode will the DNA migrate and why?
What is the role of Polyvinylpyrrolidone in DNA isolation? A. Cell lysis B Remove polyphenols C Inhibit enzymes D Create an aqueous layer where DNA stays
a. Write the reaction that proves that UV attacks DNA.b. What is a base stacking interaction? How does it help DNA?c. Explain the Sanger method in detail.
in Cohen-Boyer's recombinant DNA procedure _______ must be used for both the bacterial DNA and the amphibian DNA _______. a. the same restrictions enzyme, so that the the restriction site are identical in the DNA of each species b. different restriction enzymes, so that the genes outside the restriction site are maintained c. the same restriction enzymes, to ensure that the newly formed DNA can replicate d. different restriction enzymes, to ensure that the newly introducted genes are maintained in the bacterial DNA
Which of the folllowing is the term used for ultra compact form of DNA coiling? a. supercoiling b.metacoiling c. ultracoiling d. telephone coiling
What was the purpose of the sodium chloride in nucleic acid extraction? CHOOSE ALL ANSWERS THAT APPLY A Sodium chloride binds mostly to the proteins associated with the DNA. B Sodium ions bind to the phosphate groups in DNA. C Chloride ions bind to the phosphate groups in DNA. D Sodium chloride shields the DNA protecting it from degrading, E Sodium ions bind to the deoxyribose in the DNA.