alignment in genomic research, list types of alignments and corresponding errors associated with specific sequence assemblies. Are there methods of alignment error filtering?
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alignment in genomic research, list types of alignments and corresponding errors associated with specific sequence assemblies. Are there methods of alignment error filtering?
Introduction :-
Alignment is a fundamental technique used in genomic research to compare and match nucleotide or protein sequences.
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Solved in 2 steps
- Why a multiple sequence alignment is needed for researchers? What inferences can be derived from this kind of sequence alignments? Explain two extreme cases that are non-informative for the multiple sequence alignment.What is meant by the term "alignment" in genomic research and in what ways does alignment aid sequence assembly? How can relying on alignment lead to potential errors?Repeated sequences can be classified according to theirorganization in the genome as well as according to theirfunction. Give at least two examples of each.
- Describe the difference between Sanger based sequencing and Next Generation Sequencing (NGS). Why is NGS advantageous over Sanger based sequencing?What are the similarities and/or differences between interpreting Multiple Sequence Alignment (MSA) of conserved sequences at the DNA level versus the Amino Acid level? Please site references if possible and have 5+ sentences.How are the gap penalties adjusted in the ClustalW multiple sequence alignment program to improve the algorithm? Indicate the logic only.
- Analyzing Cloned Sequences What kind of information can a DNA sequence provide to a researcher studying a disease-causing gene?Calculate the expected number of times that a given 8-base-pair DNA site should be present in the E. coli genome. Assume that all four bases are equally probable. Repeat for a 10-base-pair site and a 12-basepair siteIn reversible terminator sequencing, how would the sequencing process be affected if the 3′-end-blocking group of each nucleotide were replaced with the 3′-H present in the dideoxynucleotides used in Sanger sequencing?
- write down the applications of pair wise and multipal sequence alignment in the field of bioinformatics. Enlist some softwares used to perform alignment. Plz enlist at least 10 to 15 points for applicationbased on the picture What is the length in basepairs of these sequences? How many base substitutions are there between these sequences? Count ONLY the instances where there is ANY nucleotide difference between any of the sequences (leave out any indels in this count) Are there any indels in this alignment? (yes or no)Explain how substitution matrices can be used to improve the possibility of identifying related protein sequences.