Amino acid sequence analysis of all of the peptides found in a single IgG antibody would reveal unique peptide sequences totaling ~600–700 amino acids. Using this estimate, the predicted molecular weight of an antibody protein would be ~70–75 kDa. Yet, an intact antibody protein has a molecular weight of ~150 kDa. The explanation for this discrepancy is:IgG antibodies have many more heavy amino acids in them than most other proteins.Each IgG antibody is a complex of two identical light chains and two identical heavy chains.IgG antibodies tend to aggregate together during purification, thereby distorting molecular weight estimates.Each IgG antibody is a complex of four identical polypeptides.IgG antibodies are produced as dimers of two identical IgG monomers.

Antibodies are made of immunoglobulin (Ig) units. They are associated with the immune system and are…

Answered: Amino acid sequence analysis of all of…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Amino acid sequence analysis of all of the peptides found in a single IgG antibody would reveal unique peptide sequences totaling ~600–700 amino acids. Using this estimate, the predicted molecular weight of an antibody protein would be ~70–75 kDa. Yet, an intact antibody protein has a molecular weight of ~150 kDa. The explanation for this discrepancy is:

IgG antibodies have many more heavy amino acids in them than most other proteins.
Each IgG antibody is a complex of two identical light chains and two identical heavy chains.
IgG antibodies tend to aggregate together during purification, thereby distorting molecular weight estimates.
Each IgG antibody is a complex of four identical polypeptides.
IgG antibodies are produced as dimers of two identical IgG monomers.

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