You are trying to purify Protein 1 from a mix of 4 different proteins without denaturing the protein. Protein 1 has a molecular weight of 3,000 Daltons and is very rich in lysine (Lys: K) Protein 2 has a molecular weight of 30,000 Daltons and is very rich in aspartic acid (Asp: D) Protein 3 has a molecular weight of 300,000 Daltons and is very rich in arginine (Arg: R) Protein 4 has a molecular weight of 30,000 Daltons and is very rich in histidine (His: H) What type of matrix/technique would make the most sense to use? NH, CH, NH NH, C-NH, NH CH2 CH, H,N*-C-C NH CH2 CH2 H,N-C CH, CH, CH2 H,N-C- H,N-C H. H. H Aspartic acid (Asp or D) Histidine Lysine (Lys or K) Arginine (Arg or R) (His or H) Oon exchange chromatography Oan affevity matrix that binds to protein 3 only Oion exchange chromatography Oan affinity matrix that binds to protein 3 only OSDS-poly acrylamide gel electrophoresis Oan affinity matrix that binds to all 4 proteins equally Ogel filtration

Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter9: Gene Expression And Gene Regulation
Section: Chapter Questions
Problem 14QP: How many kilobases of the DNA strand below will code for the protein product?
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You are trying to purify Protein 1 from a mix of 4 different proteins without denaturing the protein.
Protein 1 has a molecular weight of 3,000 Daltons and is very rich in lysine (Lys; K)
Protein 2 has a molecular weight of 30,000 Daltons and is very rich in aspartic acid (Asp: D)
Protein 3 has a molecular weight of 300,000 Daltons and is very rich in arginine (Arg: R)
Protein 4 has a molecular weight of 30,000 Daltons and is very rich in histidine (His: H)
What type of matrix/technique would make the most sense to use?
NH,
CH2
CH2
CH2
NH
NH2
C-NH,
NH
NH
CH,
H,N-C
CH,
CH2
CH2
H,N-C
H,N*-C-
H.
H.
H,N-C
C
H
H
Aspartic acid
(Asp or D)
Arginine
(Arg or R)
Histidine
Lysine
(Lys or K)
(His or H)
Olon exchange chromatography
Oan affnity matrix that binds to protein 3 only
Oion exchange chromatography
Oan affinity matrix that binds to protein 3 only
OSDS-poly acrylamide gel electrophoresis
Oan affinity matrix that binds to all 4 proteins equally
Ogel filtration
Transcribed Image Text:You are trying to purify Protein 1 from a mix of 4 different proteins without denaturing the protein. Protein 1 has a molecular weight of 3,000 Daltons and is very rich in lysine (Lys; K) Protein 2 has a molecular weight of 30,000 Daltons and is very rich in aspartic acid (Asp: D) Protein 3 has a molecular weight of 300,000 Daltons and is very rich in arginine (Arg: R) Protein 4 has a molecular weight of 30,000 Daltons and is very rich in histidine (His: H) What type of matrix/technique would make the most sense to use? NH, CH2 CH2 CH2 NH NH2 C-NH, NH NH CH, H,N-C CH, CH2 CH2 H,N-C H,N*-C- H. H. H,N-C C H H Aspartic acid (Asp or D) Arginine (Arg or R) Histidine Lysine (Lys or K) (His or H) Olon exchange chromatography Oan affnity matrix that binds to protein 3 only Oion exchange chromatography Oan affinity matrix that binds to protein 3 only OSDS-poly acrylamide gel electrophoresis Oan affinity matrix that binds to all 4 proteins equally Ogel filtration
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