An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel, while 10 ul of the DNA samples plus an appropriate amount of 6x loading buffer were added into the gel. Shown below is the AGE profile and the needed marker details where M is the MW marker while numbers 1-4 indicate the lanes with the DNA samples: м 1 M 2 3 4 ng/0.5 µg bp 20 5000 20 4000 40 3000 20 2000 20 1000 60 500 20 100 Determine the amount of the total DNA ladder added in the gel in terms of ug
Q: COMPONENT 1 REACTION N+1 REACTIONS 15.80 µL 2.50 µL 1.50 µL 2.00 µL 1.00 µL 1.00 µL 0.15 µL…
A: Polymerase chain reaction is a procedure that is used to amplify a particular segment of DNA. This…
Q: A ssDNA solution is diluted by taking 3uL of a stock solution, which is made up to 60uL with water.…
A: Beer-Lambert law relates the attenuation of light to the properties of the material through which…
Q: 1. a) A technologist has a 20µl sample of DNA and adds 5µl of loading dye before adding the total…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: In order to determine the purity of a DNA sample. spectrophotometry can be carried out at…
A: DNA absorbs the UV light at an absorbance of 260nm of the wavelength.
Q: 1000bg 800b 500bd 300bd 100bg Identify the sizes of the DNA bands in the agarose gel above using the…
A: Agarose gel electrophoresis is a separation technique mostly used for DNA molecules. Where a sample…
Q: Identify and match the possible cause(s) of the following unexpected results in agarose gel…
A: * Gel electrophoresis is an method used to separate mixtures of DNA and RNA, or proteins based on to…
Q: Hyrolysis of DNA Test Results 1. Inorganic Phosphate 2. Purines 3. Deoxyribose
A: Hydrolysis of DNA will cause the DNA backbone to break down and ultimately give the deoxyribose…
Q: An AGE run was set at 100V for 30 min. The 3 ul of the ladder was loaded into the gel, while 10 ul…
A: Ans-. The volume of the sample to be loaded into the gel is = 10ul While the given concentration of…
Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…
A: DNA (Deoxyribonucleic acid) and RNA are two examples of nucleic acids (Ribonucleic acid).…
Q: An AGE run was set at 100V for 30 min, where 3 ul of the ladder (100 ng/ul) was loaded into the gel,…
A: Lane 1 bottom band has below 500 basepairs. Lane 1 top band has 5000bp. Lane 2 band 1000bp.
Q: samples Marker B 1,200 bp 1,000 bp 900 bp 800 bp 700 bp 600 bp s00 bp 400 bp 300 bp 200 bp 100 bp…
A: The given diagram is a representation of the process of gel electrophoresis. It is a laboratory…
Q: when measuring the purity of a DNA sample? Please select the single answer that is most correct.
A: An instrument called spectrophotometer is able to measure the absorbance of specific wavelength of…
Q: A 3000 bp circular DNA was treated with both HindIII and EcoRI restriction enzymes. EcoRI cuts at…
A: In this question, we are given two restriction enzymes EcoRI and HindIII with their restricting…
Q: In automated sequencing, you are given a printout of the sense strand of your DNA. The printout is…
A: Introduction Nucleic Acids:- Nucleic acids are biopolymers, macromolecules, essential to all known…
Q: Based on this AGE profile, which of the following statements is NOT TRUE? M Uncut Ndel Ndel (Pure)…
A: Electrophoresis is a method that uses an electric field to separate macromolecules in a fluid or gel…
Q: Based on the restriction enzyme specificities given below, what was the enzyme utilized to produce…
A: The corner stone of genetic engineering or recombinant DNA technology is a special class of enzymes…
Q: Horizontal sequence :VIRL Vertical sequence:MKF Scoring rules: g/o = -3, g/e = -1, match or…
A: In bioinformatics, the Substitution matrix is a method of finding the rate of similarity of a…
Q: True or false: During DNA isolation, ice cold 95% ethanol is used to digest the DNA into fragments…
A: During DNA isolation 95% ethanol is used for DNA precipitation not for DNA digestion and while we…
Q: The figure illustrates the results of RT-qPCR. Which of the following statements about the graph is…
A: Quantitative ECR is the type of PCR in which the amount of PCR product can be determined in real…
Q: A DNA solution of unknown concentration was subjected to quantitative analysis. For better accuracy,…
A: The DNA concentration of a solution can be calculated by taking absorbance at 260nm as DNA absorbs…
Q: thanol has been carried over into your DNA sample (two answers possible here, select both of them)…
A: DNA is not soluble in ethanol . But when ethanol is there DNA gets separated by forming white…
Q: When running a gel during a Sanger sequencing, the following results were obtained: I I Find the DNA…
A: Sanger sequencing is a technique which is used to determine the order of the four nucleotide bases…
Q: A purified piece of DNA of 1650bp is cut with Hind III and Pst I separately and then with both…
A: Restriction enzymes are endonucleases derived from bacteria that make a double-stranded cut in the…
Q: Horizontal sequence :RIVL Vertical sequence:FMK Scoring rules: g/o = -3, g/e = -1, match or…
A: Substitution matrix are of two types called BLOSUM and PAM. Both these Substitution matrices are…
Q: Refer to the DNA profiles comparing the DNA obtained from the three suspects with the crime-scene…
A: The process of DNA Fingerprinting id best described as the process or technique which is used to…
Q: In which reagent is extracted DNA suspended to put it in solution? A. Sodium citrate saline buffer…
A: In which reagent is extracted DNA suspended to put it in solution? A. Sodium citrate saline…
Q: The following DNA fragment was sequenced by the Sanger method. The red asterisk indicates a…
A: DNA polymerase is an enzyme that catalyzes the synthesis of DNA molecules from nucleoside…
Q: Lane 1 Lane 2 Lane 3 Sample2: PBR3222 DNA Mass Base Pairs (ng/Sul) Sample1: TUC19 1988 :8888 4.000…
A: Agarose gel electrophoresis is a technique which is used to separate the DNA on the basis of their…
Q: In your forensic laboratory, you set up a reaction to digest DNA with restriction enzymes. The final…
A: In molecular cloning techniques like PCR and restriction cloning, restriction enzyme digestion is…
Q: An AGE run was set at 100V for 30 min. The 3 ul of the ladder was loaded into the gel, while 10 ul…
A: Gel loading buffers are important for running samples in DNA electrophoresis. The purpose of gel…
Q: The table below shows the percentage of the nitrogenous bases present in each sample. Which of the…
A: The nitrogenous bases are the part of nucleotide the nucleic acid. A, T, G and C are four…
Q: Complete this Master Mix table for 8 DNA samples, a positive control, negative control, and an extra…
A: Volume per tube (uL) number of reactions volume in master mix (uL) PCR buffer 1.2 11 13.2 dNTP…
Q: Indicate true or false for the following statements The glycerol used in the DNA loading dye…
A: Gel electrophoresis is a method that uses the electric current to push the molecules like DNA, and…
Q: You have a 2.5 ng/uL DNA stock. What volume will give you 1.15 mg?
A: Conversion tables are important to find out the actual values in the required unit. Similar…
Q: 100 F В A 75 80 85 90 95 100 105 110 115 120 125 Temperature (°C) A) What is the tm of the most…
A: DNA is a double-stranded helical molecule. The monomer of DNA is a nucleotide. A nucleotide…
Q: You are handling a paternity lawsuit brought against five poten woman. You isolated DNA from the…
A: Answer. DNA fingerprinting DNA fingerprinting also known as DNA typing or DNA profiling is a…
Q: Gel analysis practice 1) Label in the below picture and indicate the size of each DNA fragments of…
A: Gel electrophoresis is a technique which is used to separate the molecules based on the size of the…
Q: In lane 1, a size standard was loaded, which contained a mix a DNA fragments known to be 1000 bp,…
A: Gel electrophoresis is a gel technique that uses an applied electrical field to separate nucleic…
Q: In lane 1, a size standard was loaded, which contained a mix a DNA fragments known to be 1000 bp,…
A: Introduction Comparison between the location and size of the standard and human samples dictates the…
Q: You have two DNA samples, A and B. A 1/10 dilution of sample A had an absorbance at 260nm of 0.12. A…
A: Nucleic acids strongly absorb UV light with wavelengths of 260 nm due to the resonance structure of…
Q: Results obtained from a Nano Spec. DNA samples collected: ng/uL A260/A80 A260/A230 E.coli genomic…
A: Beer-Lambert Law is applied in absorption spectrometry for biochemical analysis of various…
Q: How many different loci does the FBI use for DNA profiling (fingerpriniting)?
A: Introduction: DNA fingerprinting is used to find the connection between criminal activity by…
Q: DNA bands from each sample lane of the gel electropherogram below.
A: Key points: Gel electrophoresis is a technique used to separate DNA fragments according to their…
Q: How we prepared the sample of DNA and what kinds of tracking dye we add to sample in SDS-PAGE ?…
A: INTRODUCTION SDS PAGE or Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis is a technique…
Q: Wells Base pairs: 6650 4973 4.3cm 3148 2396 1585 8 Ocm 692 > 10 Acm DNA size Markers Vector-EcoRI…
A: Hi, Thanks For Your Question. Answer : First, Let Us See The Ladder Size. Let's Do Rounding Off…
Q: . Draw the melting curve for primer annealing, labeling the axes. Label the position of the…
A:
Q: Figure 4 shows the results from Nanodrop quantification of DNA sample. Sarmple ID E Col (K-12)…
A: DNA is the genetic material in all the living organisms.
Q: DNA samples were then run in agarose gel electrophoresis against a molecular standard of 100 bp and…
A: The procedure for isolation of genomic DNA is aimed at obtaining highly pure genomic DNA that can be…
Q: The results of gel electrophoresis of 4 different DNA samples given in the figure. 16 ul was loaded…
A: Introduction: Agarose gel electrophoresis (AGE) is a method employed in molecular biology,…
Q: In order to determine the purity of a DNA sample. spectrophotometry can be carried out at _______ to…
A: DNA yield and purity is measured by absorbance. Absorbance is measured by spectrophotometer.
Step by step
Solved in 2 steps
- What are the x and y values that you have input in the excel? I thought the x would be from the concentation and the y is from the A595 of sample minus A595 of blank but the linear equation that shows up in mine is different. It's y = 0.0443x + 0.0148.An AGE run was set at 100V for 30 min. The 3 ul of the ladder was loaded into the gel, while 10 ul of the DNA samples plus an appropriate amount of 6x loading buffer were added into the gel. Find the amount of the 6x loading buffer added to 10 ul DNA samples in order to make the samples sink in the gel? Tip. From 6x final conc of the loading buffer should be 1x and answer shoud be in ulAt the station there is a 2 mL microcentrifuge tube containing sheep blood and 3 tubes containing the following solutions: 0 mM, 300 mM, 600 mM of sucrose. The tubes are randomly labelled A, B, C. 2 mL of each solution were transferred to a different microcentrifuge tube, 20 ul of blood were then added to each tube, and they were mixed well by inverting the tube for multiple times. They were observed and it was determined whether the solution in each tube was hypotonic (Yes or No) relative to the blood cells. In addition, the solutions were diluted with water to help you determine which solution is which sucrose concentration. What sucrose concentration corresponds with each tube? Tube Hypotonic (Yes/No) Hypotonic after 1:1 dilution with water (Yes/No) Most likely Sucrose concentration (mM) A No Yes B Yes Yes C No No
- Which precise standards are you going to apply in order to identify your unknown? Make sure your RFP can be clearly distinguished from the other two using the criteria. lab intro attcehd if needed:I have a 1.270 ug/uL dna stock, how would you dilute this to 50ng/nL in a single dilution step? show the exact process including the volume of water. Indicate if you would use p20,p200,p1000 for each reagant added. you can choose a total volume but cannot be less than 3 uL or exceed 1000uL. Thank you!!A 100ml dsDNA solution prepared via 1:20 dilution was taken for spectrometry analysis. The A260 and A320 values obtained were 0.4210 and 0.1210 respectively. The total DNA yield of the solution in micrograms would be 40000 300 30000 400
- Amazingly, the sensitivity of STR profiling requires only ___________ DNA-bearing cells to obtain an STR profile.DNA samples were then run in agarose gel electrophoresis against a molecular standard of 100 bp and 1Kb Ladder. The figure is analyzed and labelled manually and recorded with the used product ladder information. Figure 2A is labeled with corresponding lane numbers and with M1 and M2 as markers used. Write an interpretation of the documented gelresult (Figure 2). Note: Agarose gel of isolated DNA (A) from plant (lanes 1 & 2), chicken (lanes 3 & 4), and E. coli (lanes 5 & 6) with Vivantis* product information of 100bp (B) and 1kb ladders (C).A 10-2 is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 61 colonies of bacteria grow on the plate. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture?
- You have performed a serial dilution of an unknown sample and counted 73 CFU on a countable plate that was marked at 10^-4 dilution and you used 0.1 mL to inoculate the plate. What is the population of the original sample?Horizontal sequence :RIVL Vertical sequence:FMK Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below. SW algorithm. 1. Complete the scoring matrix. Scoring matrix with PAM250 scores: R I V L F M K 2. Set up, initialize and complete the SW matrix. 3. Retrace, align and score alignment(s). Use the arrows and circles for the matrix and path(s). R I V L F M K Align and score all optimal alignments here. PLZ the arrows and circles for the matrix and path(s) AND SHOW ALL possible Alignment Here the following points…If the purity of DNA sample is below 1.8 A260/A280, where did the protein contamination come from?