True or false: During DNA isolation, ice cold 95% ethanol is used to digest the DNA into fragments that can be collected for running in an electrophoresis gel. O True O False
Q: There can be a quantitative determination of the degree of supercoiling in a DNA sample. A True B…
A: DNA supercoiling is important for DNA packaging in the cells.
Q: There can be a quantitative determination of the degree of supercoiling in a DNA sample. True…
A: True. Digital polymerase chain reaction(dPCR) and flow cytometry could be to quantify DNA number…
Q: True or False. During gel electrophoresis, small DNA fragments are found towards the top of the gel…
A: Gel electrophoresis is a technique that uses size and charge to separate and analyze macromolecules…
Q: Hyrolysis of DNA Test Results 1. Inorganic Phosphate 2. Purines 3. Deoxyribose
A: Hydrolysis of DNA will cause the DNA backbone to break down and ultimately give the deoxyribose…
Q: The primers used in PCR are double stranded DNA oligosaccharides. True False
A: The answer is False. Primers are small, chemically synthesised oligonucleotides that are…
Q: TRUE OR FALSE. a) The Sanger method of DNA sequencing follows the principle of complementarity just…
A: The standard DNA sequencing technique is the Sanger method, which was developed by Frederick Sanger…
Q: During gel electrophoresis, DNA fragments are separated such that the longest DNA samplas migrate…
A: It is false The true statement is- during gel electrophoresis DNA fragment are separated such that…
Q: DNA QUANTITATION Consult related references (textbooks or research articles) and list possible…
A: DNA QUANTITATION: Contaminants that can affect the purity of the DNA sample are as follows:
Q: When you are extracting DNA one of the first steps is to dissolve the cell membranes with ice cold…
A: Gene expression: The process in which genetic information encoded in genes is expressed in the form…
Q: L 1 2 3 Locus A Locus B L = Standard marker 1 = Suspect 1 2 = Suspect 2 3 = DNA from crime scene %3D…
A: The biotechnology is a branch of biology that deals with different techniques that help…
Q: di-deoxy nucleotides terminate DNA elongation in Maxam-gilbert method. True False
A: First-generation DNA sequencing methods include Maxam–Gilbert sequencing and the Sanger method.…
Q: There are potential risks in performing recombinant DNA research on humans and plants due to the…
A: Recombinant DNA technology has been successfully employed to create human proteins in microbes, such…
Q: Which of the following is most needed to determine the DNA quantity using the eyeball method? -DNA…
A: DNA ladder is the most needed thing to determine the DNA quantity using the eyeball method. As we…
Q: What are the roles of the following reagents in DNA extraction? a. Ethanol b. NaCl c. SDS d. TE…
A: A) The initial role of the ethanol and monovalent cations is to remove the solvation shell…
Q: The technique that Rosalind Franklin used to take images of DNA is called NMR. O a. False O b. True
A: Nucleic acids are polynucleotides that contain many nucleotides that are bound by phosphodiester…
Q: Amazingly, the sensitivity of STR profiling requires only ___________ DNA-bearing cells to obtain an…
A: Deoxyribonucleic acid or DNA is the hereditary material that is present in all organisms. DNA…
Q: Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA…
A: DNA extraction procedure refers to the methodology that is followed to purify the the DNA (cellular…
Q: ripe banana a better sample compared to an unripe banana for DNA extraction
A: Raw bananas are often preferred for DNA extraction because they have high number of cells per…
Q: In order to determine the purity of a DNA sample, spectrophotometry can be carried our at Answer to…
A: Spectrophotometry is the quantitative analysis of spectra to compare the relative absorption or…
Q: Each PCR cycle has three steps: DNA sample denaturation, primer annealing, and elongation/extension…
A: Polymerase chain reaction (PCR) is a simple technique to amplify a DNA template to produce specific…
Q: Aside from the sample and assuming proper AGE protocol, which of the following is most needed to…
A: Agarose gel electrophoresis is an important technique used in molecular biology research. In case…
Q: All of the following are examples of materials that are bound by your purifying medium during the…
A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…
Q: Describe the characteristics of the extracted DNA, such as colour, shape, size, and consistency.
A: Deoxyribonucleic acid (DNA) is a genetic information-encoded molecule. This information is used to…
Q: When running a gel during a Sanger sequencing, the following results were obtained: I I Find the DNA…
A: Sanger sequencing is a technique which is used to determine the order of the four nucleotide bases…
Q: What will happen to the concentration and the A260/280 ratio of the DNA sample in the following…
A: Biotechnology is the use of our understanding of biological processes to develop useful applications…
Q: The production in a pcr reaction is: nucleotids double strand circular dna double stranf linear…
A: PCR stands for polymerase chain reaction.
Q: The proteins and other substances that bind to the DNA rely mostly on non-covalent interaction to…
A: Introduction Proteins are an important class of biomolecules that are found in all living organisms…
Q: Fill in the blanks: In electrophoresis, an electric current is applied to the matrix of a gel-like…
A: Gel electrophoresis is a technique that is utenly used in molecular biology laboratories. It…
Q: Someone broke into the bookstore and stole thousands of dollars’ worth of textbooks. Some hairs were…
A: Most likely the culprit is suspect 1. This is so as the banding pattern of suspect 1 is matching…
Q: What happens if you forget to add Sybrsafe to a gel that will be used for DNA gel electrophoresis…
A: Gel electrophoresis is a gel technique that separates DNA and proteins based on their mass, by means…
Q: The solid matrix that is usually used to attach the target DNA in southern blotting technique is…
A: Blotting is utilized in atomic science for the distinguishing proof of proteins and nucleic acids…
Q: Complete this Master Mix table for 8 DNA samples, a positive control, negative control, and an extra…
A: Volume per tube (uL) number of reactions volume in master mix (uL) PCR buffer 1.2 11 13.2 dNTP…
Q: Indicate true or false for the following statements The glycerol used in the DNA loading dye…
A: Gel electrophoresis is a method that uses the electric current to push the molecules like DNA, and…
Q: Briefly describe the function of each of the following components of the DNA extraction protocol.…
A: Hi, Thanks For Your Question. 1. Function Of Heat In DNA Extraction : Answer : Heating Aids In The…
Q: hematic diagram for the procedure in doing DNA extraction, isolation and quantification
A: The deoxyribonucleic acid isolation method frees deoxyribonucleic acid from the cell and {so}…
Q: The sample furthest to the left contains the DNA size standards. What is the purpose of this sample?…
A: Gel electrophoresis is an analytical technique in which DNA fragments in a sample are separated…
Q: Which of the following is not true about microarrays? Group of answer choices A microarray is a…
A: Introduction :- DNA microarray ( also known as gene chip , DNA chip, bioarray or gene array ) is an…
Q: These are the questions choices are given below (i) What is the purpose of adding table salt (NaCl)…
A: *DNA IS POSITIVELY CHARGED. *Protiens ( histones) are NEGATIVELY CHARGED *Adding NaCl or table…
Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…
A: DNA generally stands for Deoxyribonucleic acid It is generally double standard Purine and…
Q: Which of the given statements is correct in the context of visualizing DNA molecules separated by…
A: In Agarose Gel electrophoresis DNA fragments are separated under size. The negatively charged DNA…
Q: Results obtained from a Nano Spec. DNA samples collected: ng/uL A260/A80 A260/A230 E.coli genomic…
A: Beer-Lambert Law is applied in absorption spectrometry for biochemical analysis of various…
Q: Identify the component of the solution which is the extracted DNA
A: Everything living contains DNA. This DNA can be isolated for a variety of molecular biology…
Q: The proofreading of DNA is essential for faithful replication. True False
A: The process of replication is carried out with the help of a number of enzymes such as helicase, DNA…
Q: For a DNA sample, the OD value at 260nm = 0.125, and the OD value at 280nm 0.065. %3D Purity of DNA…
A: Purity of a DNA sample is defined as the ratio of absorbance at 260 nm and 280 nm. If the ratio…
Q: DNA bands from each sample lane of the gel electropherogram below.
A: Key points: Gel electrophoresis is a technique used to separate DNA fragments according to their…
Q: The Evidence shows which suspect was at the crime scene? ( image of murder investigation) A)…
A: DNA bands can appear thick and thin in the agarose gel electrophoresis, thicker and darker DNA bands…
Q: L 1 2 3 Locus A Locus B L = Standard marker 1 = Suspect 1 2 = Suspect 2 3 = DNA from crime scene
A: In biotechnology different techniques have been used for the identification of DNA among different…
Q: The first base shown in this chromatogram is closest to to : : : : 71 MW Select an answer and…
A: Asked : Correct option for 1st base in the chromatogram is closest to which end of DNA.
Q: The concentration of digested and cleaned DNA is found to be 5μg/mL. How much of your DNA sample (in…
A: Answer: DNA = (Deoxyribonucleic Acid) It is a double stranded , constitutes as the genetic material…
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- DNA Profiles as Tools for Identification A PCR-based paternity test is conducted using STRs that consistently produce a unique DNA fragment pattern from a single chromosome. Examining the results of the following Southern blot, which male(s) can be excluded as the father of the child? Which male(s) could be the father of the child?In analyzing the base composition of a DNA sample, a student loses the information on pyrimidine content. The purine content is A = 27% and G = 23%. Using Chargaffs rule, reconstruct the missing data and list the base composition of the DNA sample.True or False. During gel electrophoresis, small DNA fragments are found towards the top of the gel True False
- Gel Electrophoresis is used often in forensics. Look at the following gel to the left. From the evidence DNA, which individual matches the DNA evidence left at the crime scene?The concentration of digested and cleaned DNA is found to be 5μg/mL. How much of your DNA sample (in μL) would require to obtain a final concentration of 30ng.Gel Electrophoresis Background and Protocol: Gel electrophoresis is a laboratory technique that separates molecules by size using an electric current. The test has a positive and negative side. Do you believe DNA should be loaded on the positive (red) side or the negative (black) side? Please explain why using scientific reasoning. Will larger DNA bands be closer or farther away from the well where you administered samples? Why?
- When a DNA sample is subjected to sequencing, the two strands must be separated. True FalseThe concentration of a DNA sample is 40 nano grams per milliliter how many nanoliters will be needed to obtain 0.5 nano grams of DNACompute for the appropriate amount (mL_) of the compositions of a CTAB NaCl solution for DNA extraction. a. 10% (w/v) CTAB b. 0.7 M NaCl
- Compute for the appropriate amount (mL_) of the compositions of a 10 mL 2x CTAB buffer for DNA extraction. Answer D and E a. 2% w/v CTAB b. 1.4 M sodium chloride c. 100 mM Tris-Cl d. 20 mM EDTA e. 2% polyvinylpyrrolidone MW 40 000Make 2 mL of 50 fold dilution of DNA solution and sodium phosphate buffer. DNA: 400 uL Sodium phosphate buffer, pH 6.9, 10mL - Calclate amount of DNA & buffer need to make dilutionSimilarities between DNA agarose gels and SDS-PAGE are: Both methods use agarose gels. Both methods use electrical charge differences to separate molecules by size. Both methods determine the size of DNA fragments. All the given answers.