Animal cell culture makes use of low amounts of reagents
Q: What is the major difference between animal cell culture and plant cell culture.
A: Cell culture methods used to cultivate cells in the laboratory include animal cell culture and plant…
Q: Use the pictures below to answer the following questions. А. В. A. Is this test positive or…
A: Biomolecules These are the molecules which are produced by the cells and living organisms. These are…
Q: The colonies growing on the surface of solid media represent: Select one: a. A group of different…
A: The CFU/ml are often calculated using the formula:cfu/ml = (no. of colonies x dilution factor) /…
Q: In a Kligler Iron Agar/Triple Sugar Iron test you end up with a result with a yellow butt and red…
A: When a non-lactose fermenting bacterium hydrolyses the agar slant in the Kliger Iron Agar/ Triple…
Q: You have a culture of cells at a density of 2.7 x 109 cells/mL and you want to plate out 100 cells…
A: We shall be performing a dilution calculation for this question. We know, C1V1 = C2V2 C1 is the…
Q: lly Monella found 344 bacterial colonies on one of her plates. She had prepared this plate after a…
A: CFU is an abbreviation for Colony Forming Unit. It is used to calculate the number of viable…
Q: What is the importance of employing aseptic techniques? Give an example of a situation in the…
A: Aseptic technique:- it is a set of routine procedures that is done to prevent sterile solutions and…
Q: Place the following in the correct order in terms of steps taken, followed by the consideration…
A: DNA extraction consists of three simple steps: lysis, precipitation, and purification.
Q: Can a bacterium be positive for both MR and VP? explain
A: MR(Methyl Red) test is performed to detect glucose fermenting bacteria. The MR test acts as a pH…
Q: Koch's postulates include all of the following EXCEPT O 1) Isolate the organism in pure culture. The…
A: Koch's postulates are a series of findings and experimental criteria suggested in the late 1800s by…
Q: Which of the following is/are correct? a. Stained preparations of bacterial cells reveal much more…
A: Gram staining is used to identify bacteria. It is used to find bacterial infection in the cell.
Q: Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture…
A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…
Q: Would heating the culture in a sealed, small-diameter tube that is totally immersed in a water bath…
A: Microbial culture is a process of multiplication of microbial organisms by allowing them to…
Q: Place the following in the correct order in terms of steps taken, followed by the consideration…
A: The step and consideration are arranged in a sequence.
Q: In the petri dish in Figure 1, the bacteria Esherichia coli (E. coli) was grown and treated with…
A: The disk diffusion technique is used to estimate the susceptibility of the organism to a particular…
Q: You are testing a potential new antibiotic for its effectiveness at killing Streptococcus…
A: Drugs are medications that are taken in the prescribed amounts under the supervision of doctors to…
Q: Show the calculations required to make up 250mlof a stock solution of this chemical that will then…
A: Introduction :- Dilution is the process through which we can make a solution less concentrated by…
Q: Attached below is the study about the effects of handwashing and the effectiveness of soap against…
A: From the figure 8 , Agar plate with bacteria; (A) depicts the results before washing hands . There…
Q: List down 3 different test animals and give samples for which purposes they can be used in testing…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Provide comparison and illustration of expected growth curves from a pure culture with the…
A: 1. The growth curve from a pure culture represents the number of live cells in a bacterial…
Q: 1.You are given an unlabeled agar plate that is reddish in color. You do not know if it is MSA or…
A: The study of all living creatures that are very small, cant be seen by the naked eye is known as…
Q: Which of the following agents if introduced into a growing culture of bacteria would halt growth but…
A: INTRODUCTION Bacteria, also called germs, are microscopic organisms not visible with the naked eye.…
Q: Why is the top part of a broth or slant culture heated in a flame immediately after removing the…
A: The correct option is d. The top part of a broth or slant culture heated in a flame immediately…
Q: 1. How is UV radiation a good type of control mechanism against microbial growth? Please explain…
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: Provide three applications of cell suspension culture
A: Suspension culture is a type of culture in which single cells or small aggregates of cells multiply…
Q: Identify Four (4) possibilities in cell culture practices that able affect the quality and yield of…
A: Cell culture is the process in biology in which various organisms and animal cells are grown in an…
Q: 5. Provided with the following data, compute the corresponding CFU/ml of the original culture.…
A: One of the techniques in microbiology for culturing the microbes is the spread plate technique. The…
Q: You are working with an intestinal bacterium of humans, which you have in pure culture (in liquid…
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Do explain. QUESTION:- If the breast cancer cells treated with CBD extract, which of the following…
A: An experimental design enlists all the necessary steps that are required to conduct a successful…
Q: What should be considered when preparing a monolayer cell culture?
A: Monolayer culture, also known as adherent culture, is a form of animal cell culture in which cells…
Q: 2. Illustrate preparation of a 1:32 dilution from 1-mL culture using 50-uL water blan
A: After dilution, the dilution factor (or dilution ratio) is used to represent how much of the…
Q: A scientist develops a new medication that is a protein compound and must be administered by…
A: Sterile solution/water are used for preparing or to dissolve lyophilized powder of medicine. this…
Q: Microbiology, are there technologies that can help make bacterial culture and sensitivity done…
A: The typical bacterial culture and sensitivity test takes around 7 days to give result, which can…
Q: 1. A bacterium is grown in an environment with tryptophan added to the medium. Describe how the…
A: Need to find how the bacteria responds to tryptophan added into the medium.
Q: Examine the given table below. What interpretation can be drawn from the data regarding the test…
A: given here is a table that depicts phenol and a test disinfectant at various dilutions, following is…
Q: What are the key considerations for the scale-up of cell culture process for the large molecule drug…
A: Well developed skills are needed to increase the batch yield and to reduce processing time for large…
Q: Need help Which of these could be used to measure the number of live bacterial cells in a liquid…
A: Bacterial cultures are used to grow the bacteria in a specific environment. A bacterial culture…
Q: You contract an infection from an unknown pathogenic organism. Your doctor conducts a biochemical…
A: Given: The person contracts an infection from an unknown pathogenic organism. The doctor conducts a…
Q: The total number of cells in a culture is counted using the trypan blue exclusion assay and is found…
A: Dilutions are used in microbiology to reduce bacterial concentrations to a needed concentration for…
Q: a) What is morphology? b) Describe the common morphological characteristics of bacteria that are…
A: Bacteria are single-celled organisms. Because of their small size, these cannot be seen through…
Q: Explain why an isolated colony and a pure culture are not the samething.
A: The isolation techniques: It consists of separation of bacterial cells from other cells from mixed…
Q: Illustrate the different solid tubed media formed 1. Deep/Butt solid tubed media 2. Slant solid…
A: The selection of media is a crucial point in determining the generation of contamination-free…
Q: Which of the following items is the least suitable for sterilization by autoclave? a. Water for…
A: An autoclave is a machine that is used to perform industrial and scientific processes that require…
Q: Which of the following is a reason to run simultaneous tests on known positive and negative controls…
A: Biochemical test are different types of test carried out to find the nature and identify unknown…
Q: Define the meaning of Suspension cell culture
A: Cell culture is a media which is used for growth of the cells.
Q: In a serial dilution, you plate 1 ml of a 1/1000 dilution of a bacterial culture and find 25…
A: Serial dilution of the samples is used to calculate the number of microorganisms in a sample.…
Q: Image A: Image B: 25 um O A. image A- scanning electron microscope, Image B- phase contrast…
A: Scanning electron microscope (SEM): It produce the image of the surface area by scanning with the…
Q: Dilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you…
A: Culture consists of appropriate substances which provides the growth to the desired organisms.
Explain the statement below:
1. Animal cell culture makes use of low amounts of reagents.
Step by step
Solved in 2 steps
- 2. A mannitol-salt agar plate was inoculated with these bacteria and is shown below. A. What type of organisms grow on this medium? B. Based on the reaction below, what can you say about the organism derived from the patient's abscess?Answer the ff with not more than two sentences: Why should Erlenmeyer flasks hold not more than 60% of their capacity? When can gelatin be used in culture media preparation? How does cotton plug prevent contamination of the medium? Why should caps of glassware be loosened first before sterilization?1. The Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization? 3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distilled
- Discuss fresh, living preparations vs fixed preparations. What are the advantages and disadvantages of each technique in a microbiology laboratory?Which of the following is similar to a block of highly purified “jello-like” substance that is not completely solid and has numerous pores throughout its structure? A. Micropipette b. Restriction enzymes c. Agarose d. Negative electrode please give correct answerWhich of the following medium would be most appropriate for the bacterial cultures from stomach? a. LB b. Selective C. Differential d. Nutient
- Can you give me an example of a specific medium in culture media and fill the information below. General Information Specific a. Name of medium: b. Physical form: c. Category based on formulation: d. Purpose/uses/function: e. List of Ingredients and concentration (per 1000 ml) f. How to prepare?Explain why it is important to follow proper precautions and use aseptic technique when working with clinical samples. Why do you think that samples from urine should be tested within 2 hours of collection, or if not possible, that the sample should be refrigerated?Which of the following statements describe a culture or molecular technique in the study of microbes? a Molecular techniques are relatively cheap. b Molecular techniques can be used for mixed cultures. c All statements are acceptable. d Microbial cultivation is costly, time requiring and labor intensive.
- Blood agar contains intact sheep red blood cells which are used to determine if a microbe has the ability to lyse these cells. This agar is properly termed A. a selective differential medium B. a complex medium C. a differential medium and a complex medium D. a selective mediummicrobiology: Aseptic Culture Techniques Lab Explain why a loop or needle is flamed before it is dipped into a tube containing a pure culture. Also, explain why it is flamed after an inoculation is completedMicrobiology Briefly describe any THREE CATEGORIES (three modes of action) of antibacterial drugs. Make sure to tell how they work and how bacteria have become resistant to them.