As in the previous question: Using BSA concentrations of 0 mcg/mL, 1 mcg/mL, 3 mcg/mL, 4 mcg/mL, and 5 mcg/mL you get the following absorptions: 00.2, 0.12, 0.32, 0.42, 0.52. You dilute your sample 1:5 and get an absorbance of 0.15. What is the concentration of your sample? O a. 1.3 mog/ml b. 6.5 mcg/ml c. 0.035 mcg/ml d. 0.175 mcg/ml
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- After creating her standard curve for absorption versus the concentration (M) of FD&C Red 40, a student found that her best fit linear line for FD&C Red 40 was y = 2,962x + 0.005. Her Kool-Aid sample had an absorbance of 0.685. If 0.543 grams of Kool-Aid powder was used to prepare an 8-fl oz cup of her assigned flavor, what is the percent by mass of FD&C Red 40 in her 8-fl oz cup?A 0.1 g amine-containing compound is dissolved in water then diluted to 100 mL. You subject it to spectroscopic analysis, in order to get the concentration of amine in this compound. Next, you get 1 mL of the previously diluted sample then dilute it again to 250 mL for measurement. Then, you fill 3/4 of a 1-cm cuvette with this diluted sample, and you run an analysis using an AAS. The recorded absorbance is 0.545 at 410 nm. What is the molecular weight of the compound? (The molar absorptivity is 1.23 x 104 cm-1 mol-1 L.)A. Copper sulfate pentahydrate is often used to teach students about Beer’s Law by making a standard curve (linear regression). The bright blue color of the solution has a peak absorbance in the red range of the electromagnetic spectrum. The absorbance value is proportional to concentration. How many grams of CuSO4 5H2O to make up 250 mL of a 0.2 M stock of this solution? B. Using M1V1 = M2V2 how many mL would a student need to measure out in graduated cylinder to make up a 50 mL (V2) solution of 0.1M, 0.05 M, 0.025M 0.0125M solutions for a standard curve at the peak wavelenth for this compound?
- A. Copper sulfate pentahydrate is often used to teach students about Beer’s Law by making a standard curve (linear regression). The bright blue color of the solution has a peak absorbance in the red range of the electromagnetic spectrum. The absorbance value is proportional to concentration. How many grams of CuSO4 5H2O to make up 250 mL of a 0.2 M stock of this solution? B. Using M1V1 = M2V2 how many mL would a student need to measure out in graduated cylinder to make up a 50 mL (V2) solution of 0.1M, 0.05 M, 0.025M 0.0125M solutions for a standard curve at the peak wavelenth for this compound? I need help with part B of this question.A calibration plot of absorbance vs concentration (ppm) was obatined with standard known Red 40 dye solution. - the slope of the best-fit straight line of the plot is 0.057 ppm^-1 - The absorbance of the dilute unknown sports drink was 0.88 What is the concentration of this dilute unknown sports drink?______ the answer is 15.4 ppm ( can you please show me how to calculate this problem)A student dissolved 16.7 mg of sunscreen in 25 mL of 2-propanol. They then diluted this stock solution by a factor of 10 and measured the UV-Vis absorption of this diluted sample as 0.54. What is the absorbance/mg of the sunscreen the student used?
- You have a solution with a concentration of 0.80 mM, and you put it in a cuvette with a 1.0cm pathlength. You read an absorbance of 0.400. What is the extinction coefficient of your analyte? Question 4 options: 0.50 mmole/L/cm 0.50 mmol/L (cm)-1 0.50 L/mmole/cm 0.50 mM cmDuring an assay of paracetamol by UV an analyst prepares a series of calibration solutions containing approximately 3-15 mcg/mL paracetamol as follows: 140.1 mg of pure paracetamol was added to a 200 mL volumetric flask. 0.1 M NaOH (50 mL) was added, the mixture well shaken and made up to volume with deionized water 10 mL of the above solution was diluted to 100 mL 1)What is the concentration of the final diluted solution in mcg/mL?(1 decimal) 2) What volume of the diluted solution is required to prepare 100 mL of a 8 mcg/mL standard solution? (1 decimal)Whagt is the purpose of the following? Separate the active ingredients in an unknown over the counter analgesic using TLC (thin layer chromatography). Make a preliminary identification of each active ingredient in an unknown tablet by comparing its Rf value(s) with the Rf values of four different known active ingredients:
- In what order would the following compounds be eluted on a silica gel TLC plate if they are spotted on the same plate and developed in a solvent mixture of 10:1 hexanes:ethyl acetate A. 1 has the highest Rf value, then 2 then 4 then 3 b. 4 has the highest Rf value, then 1 then 3 then 2 c. 3 has the highest Rf value, then 2 then 1 then 4 d. 2 has the highest Rf value, then 3 then 1 then 4Caffeine (C8H10O2N4•H2O) had an absorbance of 0.510 to 272 nm in 1.0 cm optical path in concentration dissolutions of 1.0 mg/100 ml. A 2.5 g sample of soluble coffee was diluted with water to 500 ml. If they take 250 mL, add it to 25.0 mL of 0.1 N H2SO4 and make a dilution to 500 ml. The absorbance measure at 272 nm is equal to 0.415. Calculate the amount of caffeine in grams per kg of soluble coffee present in the samplePlant pigments in spinach were separated with TLC with cellulose as thestationary phase and 80/20 diethyl ether/acetone mixture as themobile phase. If needed, the solvent structures in the lab Appendix.1. Determine the Rf values of each plant component on this TLC plate.2. Arrange the plant components from most polar to most nonpolar. Please answer both 1 and 2 (picture below is the same question attached )