Assume that you have an “X" solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric obtained data given below. measurements and

Anatomy & Physiology
1st Edition
ISBN:9781938168130
Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Publisher:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Chapter3: The Cellular Level Of Organization
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Problem 7RQ: The diffusion of substances within a solution tends to move those substances ________ their ________...
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6. Assume that you have an “X" solution that you
do not know its concentration. But you have
other X solutions with known concentrations
and you know X solution absorbs maximum
light at 575 nm. To calculate the unknown
solution concentration, you have done some
spectrophotometric
obtained data given below.
measurements
and
Prepare a standard curve by using the
a)
absorbance of known concentrations (Hint:
you can learn how to prepare a standard
curve by using Excell from youtube).
Calculate the concentration of unknown.
b)
Table 1: Absorbance of X solution (575 nm)
Concentration (mg/ml)
Absorbance
0.025
0.220
0.050
0.440
0.100
0.760
0.150
1.250
0.200
1,460
unknown
0,640
Transcribed Image Text:6. Assume that you have an “X" solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric obtained data given below. measurements and Prepare a standard curve by using the a) absorbance of known concentrations (Hint: you can learn how to prepare a standard curve by using Excell from youtube). Calculate the concentration of unknown. b) Table 1: Absorbance of X solution (575 nm) Concentration (mg/ml) Absorbance 0.025 0.220 0.050 0.440 0.100 0.760 0.150 1.250 0.200 1,460 unknown 0,640
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