At pH = 7 the wild type enzyme X has optimal activity. The H102A mutant has half the maximum activity at pH = 7? Which can be a conclusion for this data? Select all that apply: H102 acts like an acid in this reaction. H102 aids in substrate binding O H102 acts like a base in this reaction. Oa strong nucleophile is necessary for catalysis to occur.

An enzyme catalyzes a reaction in which substrateA is cleaved into two products, P and Q. In the catalytic mechanism, the enzyme converts A to a covalently-bound reaction intermediate X and product P, P then desorbs from the enzyme, and in a second chemical step, the enzyme converts the intermediate X in the EX complex to the final product Q (in EQ), which then desorbs from the enzyme E.You discover two inhibitors of this enzyme, I and J. I is a competitive inhibitor of the substrate A, and has nearly double the molecular weight of J. On the other hand, J is a mixed inhibitor of enzyme E, and its inhibitory effect on Km/ Vmax (the slope effect from the double reciprocal plot) is greater than that of 1 / Vmax (the intercept effect in a double reciprocal plot). That is Kis< Kii . At low pH, the conversion of EX to EQ is greatly slowed, kcat is decreased, and the intercept effect of inhibitor J is elevated, that is, the value of Kiiis diminished. When a high, fixed concentration of…

You are working on an enzyme that obeys standard Michaelis-Menten kinetics. You have determined the Vmax to be 0.1 mol/sec and the Km to be 2.5 mM. What would the rate of the reaction be when the substrate concentration is 20 mM? 0.09 MS-1 O 0.133 Ms-1 O 0.18 Ms ¹ 9 Ms-1 O 0.018 Ms-1 0.2 MS-1

Which of the following statements regarding the catalytic mechanism of superoxide dismutase is true? The D residue does not participate in acid-base catalysis during the course of the catalyzed reaction All of the answers are true The coordinated Zn2+ participates in stabilizing Cu²+ in the reduction-oxidation reactions to consume O₂ The Cu²+ coenzyme is coordinated by H residues, positioning it precisely for consumption of 0₂ The overall reaction consumes two equivalents of O₂ and two proton equivalents in order to produces one equivalent H₂O2