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The above molecule was subjected to acid hydrolysis then was analyzed thru qualitative tests. Indicate whether the given molecule will give a positive (+) or negative (-) result in each given test.
Identify whether (+/-)
- Test for phosphate:
- Dische Test:
- Bials Test:
- Murexide test:
- Wheeler-Johnson Test:
6. What is the transitory temperature?: Higher or lower?
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- Give at least two (2) chemical tests for amino acids/ proteins other than what were performed in this experiment. For each test, cite the respective application and describe the positive result. a.Ninhydrin Test b.Biuret’s Test c.Xanthoproteic Test d.Millon’s Test e.Sakaguchi test f.Hopkins –Cole Test g.Sulfur Reaction or Lead Acetate TestGive at least two (2) chemical tests for amino acids/ proteins other than what were performed in this experiment. For each test, cite the respective application and describe the positive result. test that have been performed a.Ninhydrin Test b.Biuret’s Test c.Xanthoproteic Test d.Millon’s Test e.Sakaguchi test f.Hopkins –Cole Test g.Sulfur Reaction or Lead Acetate TestLipids from an organic sample are extracted separately by column chromatography using the ff eluants: 1st eluant - chloroform: methanol: water (6:3:1) 2nd eluant - methanol: chloroform (9:1) 3rd eluant – petroleum ether: ethyl ether (1:1) Test results for each eluate are tabulated below. Identify the possible lipid present in each eluate. Test Reagents 1. KHSO4 2. HNO3,(NH4)3MoO4 3. CHCl3, acetic anhydride, conc. H2SO4 4. -naphthol, conc. H2SO4 5. Hydroxylamine HCl, FeCl3 6. ninhydrin reagent POSSIBLE LIPIDS: Triacylglycerol, cholesterol, sphingomyelin, lecithin
- Based on this video https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Iodine test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test.Given ß- Cyclodextrin Briefly explain its expected reaction (based on their structural formula) to the different qualitative tests for carbohydrates which are: (a) Molisch’s test (b)Fehling’s test (c) Bial’s testComplete the table below regarding the different laboratory tests done for Amino Acids and Proteins Tests Procedure (Chemical Reagents Added) Positive Results/ Observations (ex: color of solution, precipitate formation, etc) Positive for (provide details like functional group responsible) Molisch Test Benedict’s Test Barfoed’s Test Moore’s Test Bial’s Test Seliwanoff’s Test Osazone Test Fehling’s Test Tollen’s Test Mucic Acid Test Lugol’s Test/ Iodine Test Hydrolysis w/ Benedict’s Test Hydrolysis w/ Iodine Test Provide the expected results with the samples given below: For negative results just leave it blank, for positive results write “(+) then the expected observation” ex: + blue solution Samples Molisch Test Benedict’s Test Barfoed’s Test Moore’s Test Bial’s Test Seliwanoff’s Test Glucose Galactose Fructose Ribose…
- REAGENTS. Match the name of the reagent with the physiological test. Tests may require more than one answer. a. alpha-naphthol b. dimethyl-alpha-naphthylamine c. ferric chloride d. Gram’s iodine e. hydrogen peroxide f. Kovac’s reagent g. methyl red h. N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride i. potassium hydroxide (KOH) j. sulfanilic acid 2,3-butanediol fermentation catalase test mixed-acid fermentation nitrate reduction oxidase test phenylalanine deamination starch hydrolysis tryptophan degradationYour supervisor asks to make 350 mL of a 0.75% (w/v) starch solution using a 5% (w/v) stock solution. You have calcuated how much stock solution you need. But, how much water (in mL) will you need to add to the stock to achieve the correct final concentration?Qualitative and Quantitative Tests for Amino Acids and ProteinsComplete the table: Test Reagents Positive Result Positive Indication Ninhydrin test Xanthoproteic acid test Millon's test Hopkins Cole test Pauly's diazo Test Lead sulphide test Histidine test Isatin test Folin’s McCarthy Sullivan Test Nitroprusside Test
- MATCHING TYPE; Match A to B A. Acrolein tesT Benedicts test Biuret test Iodine test Barfoed’s test Molisch test Seliwanoff’s test Fehling’s test Salkowski’s test Furter-Meyer test B. A. The test for the presence of cholesterol. B. The test for reducing sugars in acidic solution. C. The test is used to detect cholesterol in a solution D. The test for the presence of starch/amylose. E. The test for presence of reducing sugars in alkali solution. F. The general test for carbohydrates. G. This method is used to detect the presence of tocopherols H. The test to distinguish monosaccharides from disaccharides I. The test for the presence of fats or glycerin. J. The test to determine ketohexose from an aldohexose.Can the iodine test distinguish between amylose and amylopectin? What would you look for?What are the possible interferences or complications in detecting and differentiating aldehydes and ketones using the following tests: 2,4-dinitrophenyl hydrazine test Benedict’s test Tollen’s test Jones’ test Iodoform test pls answer them all thank you!