Calculate the amount of each chemical required to make a 10x stock of TBE in a finalvolume of 150 mL (Table 7)Molecular weight and final concentration (mM) are given. The chemicals aredissolved in distilled waterTable 7 Preparing 10x TBEComponentTris BaseBoric AcidEDTA*MolecularWeightorConcentration121.14 g/mol1 M372.24 g/mol*Ethylenediaminetetraacetic acid10x StockAmount(grams or mL)needed for 150 mLStockConcentrationto make890 mM890 mM20 mMFinal 1xWorkingConcentrationto run gel89 mM89 mM2 mM

We know that, V×M = number of mmol Where V = volume & M = molarity of the solution.

● ● Calculate the amount of each chemical required to make a 10x stock of TBE in a final volume of 150 mL (Table 7) Molecular weight and final concentration (mM) are given. The chemicals are dissolved in distilled water Table 7 Preparing 10x TBE Component Tris Base Boric Acid Molecular Weight or Concentration 121.14 g/mol 1 M 10x Stock Amount (grams or mL) needed for 150 mL Stock Concentration to make 890 mM 890 mM Final 1x Working Concentration to run gel 89 mM 89 mM

● ● Calculate the amount of each chemical required to make a 10x stock of TBE in a final volume of 150 mL (Table 7) Molecular weight and final concentration (mM) are given. The chemicals are dissolved in distilled water Table 7 Preparing 10x TBE Component Tris Base Boric Acid Molecular Weight or Concentration 121.14 g/mol 1 M 10x Stock Amount (grams or mL) needed for 150 mL Stock Concentration to make 890 mM 890 mM Final 1x Working Concentration to run gel 89 mM 89 mM

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter13: Titrations In Analytical Chemistry

Section: Chapter Questions

Problem 13.29QAP

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