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Calculate the colony forming units (CFU) if 100 colonies grow in agar using a 0.01-mL calibrated loop
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- Show the calculations required to make up 250mlof a stock solution of this chemical that will then be at the working concentration when 1 volume of this solution is added to 10 volumes of cell culture medium.Compare and contrast bacteriocidal, bacteriostatic and bacteriolytic agents. What are their effects on the optical density (OD) and viable count of a bacterial culture, respectively?"If you count 70 colonies from a urine culture obtained using 0.001ml calibrated loop, how many colony forming units (CFU) will be reported?" 70 700 7000 70000 700000
- For an exponentially growing culture that increases from 5 *106cells/ml to 5 * 108cells/ml in 8 h, calculate g, n, and k forthis culture.If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock solution would you add to 125 ml of medium? how to calculate these kind of question in tissue culture media preperationbased on the attached photo, compute the cfu/ml of 42 colonies at plate 10^5. show the complete solution and provide explanation.
- a.Describe what makes thioglycollate medium suitable for culturing anaerobes. What would the growth patterns of Clostridium sporogenes and Micrococcus luteus be in this medium? b. In the Kligler test, why do we inoculate the surface of the agar slope and then stab into the butt of the slope? What does a pink coloured colony indicate when using MAC (MacConkey Agar)?If you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a suspension with 5 x 102 cells per ml? Show your answer using a serial dilution scheme or diagramAssume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If we can order 5 milligrams of fibronectin for $871.00, how much would it cost to have enough to use every well of the 24-well culture plate?
- If 0.1 ml of a 1 * 10−6 dilution plate contains 56 colonies, calculate thenumber of cells per ml of the original culturesolve for the cfu/ml in a given of 300 colonies at plate 10^4. show complete solution. see attached photoThe plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution. Given the formula OCD = CFU/V x D, what as the Original Colony Density?